Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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Effect of Type I Collagen on Hydroxyapatite and Tricalcium Phosphate Mixtures in Rat Calvarial Bony Defects

  • Kim, Jung-Hwan (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University) ;
  • Kim, Soung-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University) ;
  • Kim, Ji-Hyuck (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University) ;
  • Kwon, Kwang-Jun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University) ;
  • Park, Young-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University)
  • Published : 2008.02.29
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Abstract

To repair bone defects in the oral and maxillofacial field, bone grafts including autografts, allografts, and artificial bone are used in clinical dentistry despite several disadvantages. The purpose of this study was to evaluate new bone formation and healing in rat calvarial bone defects using hydroxyapatite (HA, $Ca_{10}[PO_4]_6[OH]_2,\;Bongros^{(R)}$, Bio@ Co., KOREA) and tricalcium phosphate (${\beta}-TCP,\;Ca_3[PO_4]_2$, Sigma-Aldrich Co., USA) mixed at various ratios. Additionally, this study evaluated the effects of type I collagen (Rat tail, BD Biosciences Co., Sweden) as a basement membrane organic matrix. A total of twenty, 8-week-old, male Sprague-Dawley rats, weighing 250-300g, were divided equally into a control group (n=2) and nine experimental groups (n=2, each). Bilateral, standardized transosseous circular calvarial defects, 5.0 mm in diameter, were created. In each experimental group, the defect was filled with HA and TCP at a ratio of 100:0, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 0:100 with or without type I collagen. Rats were sacrificed 4 and 8 weeks post-operation for radiographic (standardized plain film, Kodak Co., USA), histomorphologic (H&E [Hematoxylin and Eosin], MT [Masson Trichrome]), immunohistochemical staining (for BMP-2, -4, VEGF, and vWF), and elementary analysis (Atomic absorption spectrophotometer, Perkin Elmer AAnalyst $100^{(R)}$). As the HA proportion increased, denser radiopacity was seen in most groups at 4 and 8 weeks. In general radiopacity in type I collagen groups was greater than the non-collagen groups, especially in the 100% HA group at 8 weeks. No new bone formation was seen in calvarial defects in any group at 4 weeks. Bridging bone formation from the defect margin was marked at 8 weeks in most type I collagen groups. Although immunohistochemical findings with BMP-2, -4, and VEGF were not significantly different, marked vWF immunoreactivity was present. vWF staining was especially strong in endothelial cells in newly formed bone margins in the 100:0, 80:20, and 70:30 ratio type I collagen groups at 8 weeks. The calcium compositions from the elementary analysis were not statistically significant. Many types of artificial bone have been used as bone graft materials, but most of them can only be applied as an inorganic material. This study confirmed improved bony regeneration by adding organic type I collagen to inorganic HA and TCP mixtures. Therefore, these new artificial bone graft materials, which are under strict storage and distribution systems, will be suggested to be available to clinical dentistry demands.

Keywords

References

  1. Citardi MJ, Friedman CD: Nonvascularized autogenous bone grzafts for craniofacial skeletal augmentation and replacement. Otolaryng Clin N Am 1994;27:891-911
  2. Lekholm U, Wannfors K, Isaksson S, et al.: Oral implants in combination with bone grafts. Int J Oral Maxillofac Surg 1999;28:181-187 https://doi.org/10.1016/S0901-5027(99)80134-9
  3. Triplett RG, Schow SR: Autologous bone grafts and endosseous implants: complementary techniques. J Oral maxillofac Surg 1996;54:486-494 https://doi.org/10.1016/S0278-2391(96)90126-3
  4. Hill NM, Horne JG, Devane PA: Donor site morbidity in the iliac crest bone graft. Aust N Z J Surg 1999;69:726-728 https://doi.org/10.1046/j.1440-1622.1999.01674.x
  5. Broecke DGA: Neurotmesis of the lateral femoral cutaneous nerve when coring for iliac crest bone grafts. Plast Reconstr Surg 1998;102:1163-1166 https://doi.org/10.1097/00006534-199809040-00038
  6. Forrest C, Boyd B, Manktelow R, et al.: The free vascularized iliac tissue transfer: donor site complication associated with eighty-two cases. Br J Plast Surg 1992;45:89-93 https://doi.org/10.1016/0007-1226(92)90163-R
  7. Bruchardt H, Jones H, Glowczewskie F, et al.: Freeze-dried allogenic segmental cortical-bone grafts in dogs. J Bone Joint Surg 1978;60:1082-1090 https://doi.org/10.2106/00004623-197860080-00011
  8. Buck BE, Malinin TI, Brown MD: Bone transplantation and human immunodeficiency virus. An estimate of risk of acquired immunodeficiency syndrome (AIDS). Clin Orthop 1989;240:129-136
  9. Perry CR: Bone repair technique, bone graft, and bone graft substitutes. Cli Orthop Rel Res 1999;360:N71-86 https://doi.org/10.1097/00003086-199903000-00010
  10. Ohbayashi Y, Miynoru M, Nagahata S: A long-term study of implanted artificial hydroxyapatite particles surrounding the carotid artery in adult dog. Biomaterials 2000;21:501-509 https://doi.org/10.1016/S0142-9612(99)00208-2
  11. Ono I, Tateshita T, Nakajima T: Evaluation of a high density polyethylene fixing system for hydroxyapatite ceramic implants. Biomaterial 2000;21:143-151 https://doi.org/10.1016/S0142-9612(99)00141-6
  12. Breitbart AS, Staffenberg DA, Thorne CCHM: Tricalcium phosphate and osteogenin: a bioactive on lay bone graft substitute. Plast Reconstr Surg 1995;96:699-708 https://doi.org/10.1097/00006534-199509000-00024
  13. Fortunato G, Marini E, Valdinucci F, et al.: Long-term of hydroxyapatite-fibrin glue implantation in plastic and reconstructive craniofacial surgery. J Craniomaxillofac Surg 1997;25:124-135 https://doi.org/10.1016/S1010-5182(97)80003-0
  14. Andrade JCT, Camilli JA, Kawachi EY, et al.: Behavior of dense and porous hydroxyapatite implants and tissue response in rat femoral defects. J Biomed Master Res 2002;62:30-36 https://doi.org/10.1002/jbm.10242
  15. Thomas MS, Lynne AO, John AP: Repair of critical size rat calvarial defects using extracellular matrix protein gels. J Neurosurg 1995;83:710-715 https://doi.org/10.3171/jns.1995.83.4.0710
  16. Barrack RL, Cook SD, Patron LP, et al.: Induction of bone ingrowth form acetabular defects to a porous surface with OP-1 o R. Clin Orthop 2003;417:41-49
  17. Caplan AI: New logic for tissue engineering: multifunctional and biosmart delivery vehicles. In: Davies JE, ed. Bone Engineering. Toronto: Em squared, 2000:441-446
  18. Hollinger JO, Kleinschmidt JC: The critical size defect as an experimental model to test bone repair materials. J Craniofacial Surg 1990;1:60-68 https://doi.org/10.1097/00001665-199001000-00011
  19. Hing AH: Bone repair in the twenty-first century: biology, chemistry or engineering? Phil Trans R Soc Lond A. 2004;362:2821-2850 https://doi.org/10.1098/rsta.2004.1466
  20. Posner AS: The mineral of bone. Clin Orthop 1985;200:87-99
  21. Eisenberger S, Lehrman A, Turner WD: Basic calcium phosphates and related system, Subpart A. Carbonate-apatite versus hydroxyapatite. In: Chemical review Vol. 26, Johnson Reprint Corporation New York 1940:285
  22. Legros R, Balmain N, Bonel G: Age-related changes in mineral of rat and bovine cortical bone. Calcif Tissue Int 1987;41:137-144 https://doi.org/10.1007/BF02563793
  23. Peters F, Schwarz K, Epple M: The structure of bone studied with synchrotron X-ray diffraction, X-ray absorption spectroscopy and thermal analysis. Thermochim Acta 2000;361:131-138 https://doi.org/10.1016/S0040-6031(00)00554-2
  24. Posner AS, Betts F: Synthetic amorphous calcium phosphate and its relation to bone mineral structure. Accts Chem Res 1975;8:273-281 https://doi.org/10.1021/ar50092a003
  25. Tadic D, Peters F, Epple M: Continuous synthesis of amorphous carbonated apatites. Biomaterials 2002;23:2553-2559 https://doi.org/10.1016/S0142-9612(01)00390-8
  26. Le Geros RZ: Effect of carbonate on the lattice parameters of apatite. Nature 1965;206:403-404 https://doi.org/10.1038/206403a0
  27. Lowenstam H, Weiner AS: On biomineralization. Oxford University Press New York Oxford. 1989
  28. Mann S: Biomimetic materials chemistry. VCH publishers Inc. Weinheim, New York Cambridge. 1996
  29. Weiner S, Arad T, Sabanay I, Traub W: Rotated plywood structure of primary lamellar bone in the rat: Orientation of the collagen fibril arrays. Bone 1997;20:509-514 https://doi.org/10.1016/S8756-3282(97)00053-7
  30. Weiner S, Wagner HD: The material bone: structure-mechanical function relations. Annu rev Master Sci 1998;28:271-298 https://doi.org/10.1146/annurev.matsci.28.1.271
  31. Albee FH, Morrison HF: Studies in bone growth, triple calcium phosphate as a stimulus to osteogenesis. Ann Surg 1920;71:32
  32. Jarcho M: Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop 1981;157: 259
  33. Geesink RGT, De Groot K: Bonding of bone to apatite coated implants. J Bone Joint Surg 1988;70:17
  34. Byrd HS, Hobar PC: Augmentation of craniofacial skeleton with porous hyroxyapatite granules. Reconstr Surg 1993;91:15-22 https://doi.org/10.1097/00006534-199301000-00003
  35. Hollinger JO, Brekke J: Role of bone substitutes. Clin Orthop 1996;324:55-65 https://doi.org/10.1097/00003086-199603000-00008
  36. Constantino PD, Freidman CD: Synthetic bone graft substitutes. Otolaryngol Clin North Am 1994;27:1037-1073
  37. Havelin LI, Engesaeter LB, Espehaug B, Furnes O, Lie SA, Vollset SE: The Norwegian Arthroplasty Register: 11 years and 73,000 arthroplasties. Acta Orthop Scand 2000; 71:337-353 https://doi.org/10.1080/000164700317393321
  38. Bellemans J: Osseointegration in porous coated knee arthroplasty. The influence of component coating type in sheep. Acta Orthop Scand Suppl 1999;288:1-35
  39. Strnad Z, Strnad J, Povysil C, Urban K: Effect of plasma sprayed hyroxyapatite coating on the osteoconductivity of commercially pure titanium implants. Int J Oral Maxillofac Implants 2000;15:483-490
  40. Oonishi H, Kushitani S, Yasukawa E, et al.: Particulate bioglass compared with hydroxyapatite as a bone graft substitute. Clin Orthop 1997;334:316-325
  41. Paralkar VM, Nandedkar AKN, Pointer RH, et al.: Interaction of osteogenin, a heparin binding bone morphogenetic protein, with type IV collagen. J Biol Chem 1990;265:17281-17284
  42. Sampath TK, Muthukumaran N, Reddi AH: Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography. Proc Natl Acad Sci 1987;84:7109-7113
  43. Vukicevic S, Paralkar VM, Cunnungham NS, et al.: Autoradiographic localization of osteogenin binding sites in cartilage and bone during rat embryonic development. Dev Biol 1990;140:209-214 https://doi.org/10.1016/0012-1606(90)90068-T