Changes in Effective Components of Ginseng by Puffing

팽화에 의한 인삼 유효성분의 변화

  • Kim, Ji-Hye (Dept. of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, and Medical Research Institute, College of Medicine, Pusan National University) ;
  • Choi, Sung-Won (Department of Food and Culinary Arts, Osan College) ;
  • Hur, Nam-Youn (Department of Food and Culinary Arts, Osan College) ;
  • Kim, Byung-Yong (Dept. of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Baik, Moo-Yeol (Dept. of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University)
  • 김지혜 (경희대학교 생명자원과학연구원 식품공학과) ;
  • 안순철 (부산대학교 의과대학 미생물학교실) ;
  • 최성원 (오산대학교 호텔조리계열) ;
  • 허남윤 (오산대학교 호텔조리계열) ;
  • 김병용 (경희대학교 생명자원과학연구원 식품공학과) ;
  • 백무열 (경희대학교 생명자원과학연구원 식품공학과)
  • Published : 2008.09.30

Abstract

This study was conducted to investigate the effect of moisture content and pressure on extraction yield, crude saponins and ginsenoside contents of puffed Korean ginseng. Puffed ginsengs showed relatively higher extraction yield ($50.0{\sim}62.1%$) and amounts of crude saponins ($19.6{\sim}48.8$ mg/g ginseng) than no-puffed ginseng ($37.6{\pm}0.8%$ and $11.0{\pm}1.0$ mg/g ginseng), respectively. The highest extraction yield and amounts of crude saponins were obtained in 8.0% moisture content sample puffed at 10 $kg_f/cm^2$. In HPLC analysis, amounts of measured major ginsenosides (Rb1, Rb2, Rc, Rd, Re, and Rg1) decreased with increasing puffing pressure, yet contents of almost all major gin senosides were higher than control (no-puffed). On the other hand, ginsenoside Rg3 were produced after puffing suggesting that chemical structure of some ginsenosides might be altered during the puffing process. These results indicate that puffing can increase the extraction yield and crude saponin contents and it could influence the ginsenoside composition.

수삼을 수분함량 별로 건조한 다음 원형이 변하지 않는 팽화 인삼을 제조할 수 있는 최적의 압력조건을 찾아 팽화 인삼을 제조하고 각 조건 별 시료의 추출수율, 조사포닌, ginsenoside함량 변화를 관찰하였다. 팽화 처리 후 외관상의 가장 큰 변화는 갈변과 부피팽창이었다. 추출수율 측정 결과, 대조군은 37.6%, 팽화 인삼의 경우 $50.0{\sim}62.1%$의 범위로 측정되었다. 조사포닌 함량의 경우 대조군은 11.0 mg/g ginseng, 팽화 인삼은 $19.6{\sim}48.8mg/g$ ginseng의 범위로 측정되었다. 팽화 인삼에서는 홍삼 특유 사포닌인 ginsenoside-Rg3가 검출되었다. Ginsenoside-Rg3의 생성량은 팽화 압력이 증가함에 따라 유의적으로 증가하는 경향을 나타내었다. Ginsenoside-Rg3를 제외한 나머지 기본 ginsenoside와 total ginsenoside함량은 대조군에 비해 모두 증가하였지만, 팽화 압력이 증가함에 따라 그 양이 감소하는 경향을 나타내었다. 따라서 본 연구 결과 인삼을 적절한 조건에서 팽화처리하였을 경우 추출수율, 조사포닌 및 조사포닌 함량의 증진과 일부 ginsenoside의 변형을 확인할 수 있었다.

Keywords

References

  1. Li, X. G. (1992) Studies on the transforming mechanism of amino acid components in ginseng in the course of ginseng process. Korean J. Ginseng Sci. 16, 64-67
  2. Singh, V. K., Agarwal, S. S. and Gupta, B. M. (1984) Immunomodulatory activity of Panax ginseng extract. Proc. 4th Intl. Ginseng Symp
  3. Benishin, C. G. (1992) Actions of ginsenoside Rb1 on choline uptake in central cholinergic nerve endings. Neurochem. Intl. 21, 1-5 https://doi.org/10.1016/0197-0186(92)90061-U
  4. Satio, H. and Bao, T. T. (1984) Effect of red ginseng on mice exposed to various stress. Proc. 4th Intl. Ginseng Symp
  5. Kim, W. Y., Kim, J. M., Han, S. B., Lee, S. K., Kim, N. D. and Park, M. K. (2000) Steaming of ginseng at high temperature enhances biological activity. J. Nat. Prod. 63, 1702-1704 https://doi.org/10.1021/np990152b
  6. Kim, H. Y., Chen, X. and Gills, C. N. (1992) Ginsenosides protect pulmonary vascular endotheliumagainst free radical induced injury. Biochem. Biophy. Pes. 189, 670-676 https://doi.org/10.1016/0006-291X(92)92253-T
  7. Park, J. D. (1996) Recent studies on the chemical constituents of Korean ginseng. Korean J. Ginseng Sci. 20, 389-415
  8. Nam, K. Y. (2005) The comparative understanding between red ginseng and white ginsengs processed ginsengs (Panax ginseng C. A. Meyer). J. Ginseng Res. 29, 1-18 https://doi.org/10.5142/JGR.2005.29.1.001
  9. Matz, S. A. (1959) The Chemistry and Technology of Cereals as Food and Feed. AVI Publishing Company INC, Westport
  10. Chandrasekhar, P. R. (1989) Some studies on heated air fluidized bed puffing characteristics of rice. Unpublished Ph.D. Thesis, PHTC, IIT, Kharagpur (W.B.), India
  11. Kim, S. B., Do, J. R., Lee, Y. W. and Gu, Y. S. (1990) Nitritescavenging effects of roasted-barley extracts according to processing conditions. Korean J. Food Sci. Technol. 22, 748-752
  12. Court, W., Hendel, J. and Elmi, J. (1996) Reversed-phase highperformance liquid chromatographic determination of ginsenosides of Panax quinquefolium. J. Chromatogr. A 755, 11-17 https://doi.org/10.1016/S0021-9673(96)00580-8
  13. Yoon, S. R., Lee, M. H. and Park, J. H. (2005) Changes in physicochemical compounds with heating treatment of ginseng. J. Korean Soc. Food Sci. Nur. 34, 1572-1578 https://doi.org/10.3746/jkfn.2005.34.10.1572
  14. Kim, B. S. and Ryu, G. H. (2005) Effect of die temperature and dimension on extract chracteristics of extruded white ginseng. J. Korean Soc. Food Sci. Nutr. 34, 544-548 https://doi.org/10.3746/jkfn.2005.34.4.544
  15. Yoon, S. R., Lee, M. H. and Park, J. H. (2005) Changes in physicochemical compounds with heating treatment of ginseng. J. Korean Soc. Food Sci. Nutr. 34, 1572-1578 https://doi.org/10.3746/jkfn.2005.34.10.1572
  16. Lee, C. R., Whang, W.K., Shin, C. G., Lee, S. H., Han, S. T., Im, B.O. and Ko, S. K. (2004) Comparison of ginsenoside composition and contents in fresh ginseng roots cultivated in Korea, Japan, and China at various ages. J. Korean Food Sci. Technol. 36, 847-850
  17. Park JH, Kim JM, Han SB, Kim NY, Surh YJ, Lee SK, Kim ND. Park MK. (1998) 'A new processed ginseng with fortified activity' in Advances in ginseng research. 146-159, Korean Society of Ginseng, Seoul, Korea
  18. Kwon, S. W., Han, S. B., Park, I. H., Kim, J. M., Park, M. K. and Park, J. H. (2001) Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J. Chromatogr. A 921, 335- 339 https://doi.org/10.1016/S0021-9673(01)00869-X
  19. Yang, S. J., Jeong, K. S., Yoo, J. S., Kang, T. S., Noh, Y. H., Lee, J. S. and Jeong, H. S. (2006) Change of Korean ginseng components with high temperature and pressure treatment. Korean J. Food Sci. Technol. 38, 521-525
  20. Ha, D. C., Kim, B. S., Byun, E. H., Lee, J. W. and Ryu, G. H. (2004) Optimization of extrusion process variables for red ginseng manufacturing. 70th Annual Conference in Korean Society of Food Science and Technology