한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제16권4호
  • /
  • Pages.261-267
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  • 2008
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

고려인삼에서 Malate Dehydrogenase 유전자의 분리 및 분석

Isolation and Characterization of Malate Dehydrogenase Gene from Panax ginseng C.A. Meyer

  • 김유진 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 심주선 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 이정혜 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 정대영 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 인준교 ((주)바이오피아) ;
  • 이범수 ((주)바이오피아) ;
  • 민병훈 (배재대학교 생명환경디자인학부) ;
  • 양덕춘 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행)
  • Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Shim, Ju-Sun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Lee, Jung-Hye (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Jung, Dae-Young (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • In, Jun-Gyo (Biopia Co., Ltd.) ;
  • Lee, Bum-Soo (Biopia Co., Ltd.) ;
  • Min, Byung-Hoon (Life Science and Enviromental Design, Pai Chai University) ;
  • Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
  • 발행 : 2008.08.30
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초록

Malate dehydrogenase is a ubiquitous enzyme in plants, involving in a range of metabolic processes depending on its subcellular location. A malate dehydrogenase (PgMDH) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The deduced amino acid sequence of PgMDH showed high similarity with the NAD-dependent mitochondrial malate dehydrogenase from Glycinemax (P17783), Eucalyptus gunnii (P46487), and Lycopersicon esculentum (AAU29198). And the segment of a malate dehydrogenase gene was amplified through RT-PCR. The expression of PgMDH was increased after treatments of chilling, salt, UV, cadmium or copper treatment.

키워드

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