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Isolation and Characterization of Malate Dehydrogenase Gene from Panax ginseng C.A. Meyer  

Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Shim, Ju-Sun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Lee, Jung-Hye (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Jung, Dae-Young (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
In, Jun-Gyo (Biopia Co., Ltd.)
Lee, Bum-Soo (Biopia Co., Ltd.)
Min, Byung-Hoon (Life Science and Enviromental Design, Pai Chai University)
Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Korean Journal of Medicinal Crop Science / v.16, no.4, 2008 , pp. 261-267 More about this Journal
Abstract
Malate dehydrogenase is a ubiquitous enzyme in plants, involving in a range of metabolic processes depending on its subcellular location. A malate dehydrogenase (PgMDH) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The deduced amino acid sequence of PgMDH showed high similarity with the NAD-dependent mitochondrial malate dehydrogenase from Glycinemax (P17783), Eucalyptus gunnii (P46487), and Lycopersicon esculentum (AAU29198). And the segment of a malate dehydrogenase gene was amplified through RT-PCR. The expression of PgMDH was increased after treatments of chilling, salt, UV, cadmium or copper treatment.
Keywords
Panax ginseng; Malate Dehydrogenase; Abiotic Stress; cDNA; RT-PCR;
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Times Cited By KSCI : 2  (Citation Analysis)
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