Reproductive and Developmental Biology

  • 제32권2호
  • /
  • Pages.89-95
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  • 2008
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Plasminogen Activators Activities in Oviductal Epithelial Cells during Estrus Cycle in the Pig

  • Shin, Mi-Young (College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Tae-Shin (College of Animal Life Sciences, Kangwon National University) ;
  • Kwon, Eun-Hye (Korea Basic Science Institute Chuncheon Center, Kangwon National University) ;
  • Park, Soo-Bong (National Institute of Animal Science) ;
  • Park, Chun-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Dong-Seok (College of Natural Sciences, Kyungpook National University)
  • 발행 : 2008.06.30
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초록

The present study was undertaken to identify changes of plasminogen activators (PAs) in porcine oviductal epithelial cells (POECs) during the estrous cycle classified with post-ovulatory stages (Post-Ov), early to mid-luteal stages (Early-mid L) and pre-ovulatory (Pre-Ov) stages. The urokinase-type plasminogen activator (uPA) was only observed on day 5 and day 7 of culture in the POECs on all the estrous cycles and gradually increased according to increasing culture times, but not Early-mid L. In POECs-conditioned medium, uPA, tissue-type (tPA) and tPA-PA inhibitor (tPA-PAI) activity were observed at all culture times during estrous cycles. The uPA activity of POECs-conditioned medium on Post-Ov stage were significantly (p<0.05) decreased during prolonged cultures. On the other hand, the tPA activity of POECs-conditioned medium at Post-Ov stage was significantly (p<0.05) higher on day 5 than compared to the other days. Although was higher on day 1 at Post-Ov stage, the tPA-PAI activity of POECs-conditioned medium was significantly (p<0.05) higher on day 7 at all stage than that of day 5 of the culture. Taken together, these results suggest that uPA, tPA and tPA-PAI are produced by POECs, and the variations of the PAs activity are regulated in the different stages of the estrous cycle.

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참고문헌

  1. Beers WH (1975): Follicular plasminogen and plasminogen activator and the effect of plasmin on ovarian follicle wall. Cell 6:379-386 https://doi.org/10.1016/0092-8674(75)90187-7
  2. Bray BA, Hsu KC, Wigger HJ, LeRoy EC (1975): Association of fibrinogen and microfibrils with trophoblast basement membrane. Connective Tissue Research 3:55-71 https://doi.org/10.3109/03008207509152342
  3. Bruse C, Bergqvist A, Carlstrom K, Fianu-Jonasson A, Lecander I, stedt B (1998): Fibrinolytic factors in endometriotic tissue, endometrium, peritoneal fluid, and plasma from women with endometriosis and in endometrium and peritoneal fluid from healthy women. Fertility and Sterility 70:821- 826 https://doi.org/10.1016/S0015-0282(98)00285-4
  4. Buhi WC, Alvarez IM, Kouba AJ (1997): Oviductal regulation of fertilization and early embryonic development. Journal of Reproduction and Fertility Supplement 52:285-300
  5. Canipari R, Epifano O, Siracusa G, Salustri A (1995): Mouse oocytes inhibit plasminogen activator production by ovarian cumulus and granulosa cells. Developmental Biology 167:371-378 https://doi.org/10.1006/dbio.1995.1031
  6. Cannon MJ, Menino J (1998): Changes in the bovine zona pellucida induced by plasmin or embryonic plasminogen activator. Molecular Reproduction, Development 51:330-338
  7. Colgin DC, Murdoch WJ (1997): Evidence for a role of the ovarian surface epithelium in the ovulatory mechanism of the sheep: Secretion of urokinase- type plasminogen activator. Animal Reproduction Science 47:197-204 https://doi.org/10.1016/S0378-4320(97)00011-0
  8. Dano K, Andreasen PA, Grondahl-Hansen J, Kristensen P, Nielsen LS, Skriver L (1985): Plasminogen activators, tissue degradation, and cancer. Advances in Cancer Research 44:139-266 https://doi.org/10.1016/S0065-230X(08)60028-7
  9. Feigelson M, Kay E (1972): Protein patterns of rabbit oviducal fluid. Biology of Reproduction 6:244-252 https://doi.org/10.1093/biolreprod/6.2.244
  10. Finlay TH, Katz J, Kirsch L, Levitz M, Nathoo SA, Seiler S (1983): Estrogen-stimulated uptake of plasminogen by the mouse uterus. Endocrinology 112: 856-861 https://doi.org/10.1210/endo-112-3-856
  11. Foxcroft GR, Hunter MG (1985): Basic physiology of follicular maturation in the pig. Journal of Reproduction and Fertility Supplement 33:1-19
  12. Gabler C, Killian GJ, Einspanier R (2001): Differential expression of extracellular matrix components in the bovine oviduct during the oestrous cycle. Reproduction 122:121-130 https://doi.org/10.1530/rep.0.1220121
  13. Hart DA, Rehemtulla A (1988): Plasminogen activators and their inhibitors: Regulators of extracellular proteolysis and cell function. Comparative biochemistry and physiology B. Comparative Biochemistry 90:691-708 https://doi.org/10.1016/0305-0491(88)90323-9
  14. Huarte J, Vassalli JD, Belin D, Sakkas D (1993): Involvement of the plasminogen activator/plasmin proteolytic cascade in fertilization. Developmental Biology 157:539-546 https://doi.org/10.1006/dbio.1993.1156
  15. Hunter RHF, Baker TG (1975): Development and fate of porcine Graafian follicles identified at different stages of the oestrous cycle. Journal of Reproduction and Fertility 43:193-196 https://doi.org/10.1530/jrf.0.0430193
  16. Irigoyen JP, Munoz-Canoves P, Montero L, Koziczak M, Nagamine Y (1999): The plasminogen activator system: Biology and regulation. Cellular and Molecular Life Sciences 56:104-132 https://doi.org/10.1007/PL00000615
  17. Jimenez Diaz, Giunta, Valz-Gianinet, Pereyra-Alfonso, Flores, Miceli (2000): Proteases with plasminogen activator activity in hamster oviduct. Molecular Reproduction and Development 55:47-54 https://doi.org/10.1002/(SICI)1098-2795(200001)55:1<47::AID-MRD7>3.0.CO;2-J
  18. Kobayashi T, Matsuda Y, Park JY, Hara I, Kaneko S, Fujimoto Y, Nozawa S, Akihama S (1992): Trypsin- like arginine amidases including plasminogen and plasmin in human seminal plasma by affinity adsorption and elution. Archives of Andrology 28: 165-170 https://doi.org/10.3109/01485019208987694
  19. Kokolis NA (1990): Effect of orchidectomy on tissue plasminogen activator activity, plasminogen activator inhibition and plasmin inhibition. Haemostasis 20:233-240
  20. Kruithof EKO, Tran-Thang C, Ransijn A, Bachmann F (1984): Demonstration of a fast-acting inhibitor of plasminogen activators in human plasma. Blood 64:907-913
  21. Lee SL, Wei YH (1994): The involvement of extracellular proteinases and proteinase inhibitors in mammalian fertilization. Biotechnology and Applied Biochemistry 19:31-40
  22. Lippes J, Wagh PV (1993): Human oviductal fluid proteins. VI. Correlation between alpha-fetoprotein and serum levels of ovarian hormones. Fertility and Sterility 59:157-162 https://doi.org/10.1016/S0015-0282(16)55632-5
  23. Liu K, Liu YX, Du Q, Zhou HM, Lin X, Hu ZY, Zhang GY, Zhang GH (1996): Preliminary studies on the role of plasminogen activator in seminal plasma of human and rhesus monkey. Molecular Human Reproduction 2:99-104 https://doi.org/10.1093/molehr/2.2.99
  24. Mastroianni L, Go KJ (1979): The Biology of the Fluids of the Female Genital Tract. Elsevier/North Holland 335-344
  25. Rajput B, Degen SF, Reich E (1985): Chromosomal locations of human tissue plasminogen activator and urokinase genes. Science 230:672-674 https://doi.org/10.1126/science.3840278
  26. Rehemtulla A, Arndt A, Hart DA (1990): Induction of plasminogen activator inhibitor type 2 expression during differentiation of human K562 cells towards a macrophage phenotype. Biochemistry and Cell Biology 68:1337-1343 https://doi.org/10.1139/o90-196
  27. Reinthaller A, Kirchheimer JC, Deutinger J, Bieglmayer C, Christ G, Binder BR (1990): Plasminogen activators, plasminogen activator inhibitor, and fibronectin in human granulosa cells and follicular fluid related to oocyte maturation and intrafollicular gonadotropin levels. Fertility and Sterility 54:1045-1051 https://doi.org/10.1016/S0015-0282(16)54003-5
  28. Rekkas C, Kokolis N, Belibasaki S, Tsantarliotou M, Smokovitis A (2000): Effect of alpha-tocopherol on plasma testosterone and plasminogen activator activity or inhibition in ram spermatozoa. Theriogenology 53:751-760 https://doi.org/10.1016/S0093-691X(99)00272-1
  29. Rekkas C, Kokolis N, Smokovitis A (1993): Breed and seasonal variation of plasminogen activator activity and plasminogen activator inhibition in spermatozoa and seminal plasma of the ram in correlation with testosterone in the blood. Andrologia 25:101-109 https://doi.org/10.1111/j.1439-0272.1993.tb02690.x
  30. Ricke WA, Redmer DA, Reynolds LP (1999): Growth and cellular proliferation of pig corpora lutea throughout the oestrous cycle. Journal of Reproduction and Fertility 117:369-377 https://doi.org/10.1530/jrf.0.1170369
  31. Robbins KC, Summaria L, Hsieh B, Shah RJ (1967): The peptide chains of human plasmin. Mechanism of activation of human plasminogen to plasmin. Journal of Biological Chemistry 242:2333-342
  32. Roldan-Olarte M, Jimenez-Diaz, Miceli D (2005): Plasminogen detection in oocytes and plasminogen activator activities in the porcine oviduct during the estrous cycle. Zygote 13:115-123 https://doi.org/10.1017/S0967199405003175
  33. Schatz F, Lockwood CJ (1993): Progestin regulation of plasminogen activator inhibitor type 1 in primary cultures of endometrial stromal and decidual cells. Journal of Clinical Endocrinology and Metabolism 77:621-625 https://doi.org/10.1210/jc.77.3.621
  34. Schmitt M, Harbeck N, Thomssen C, Wilhelm O,Magdolen V, Reuning U, Ulm K, Fler H, Nicke F, Graeff H (1997): Clinical impact of the plasminogen activation system in tumor invasion and metastasis: Prognostic relevance and target for therapy. Thrombosis and Haemostasis 78:285-296 https://doi.org/10.1055/s-0038-1657541
  35. Smokovitis A, Kouimtzis S, Koutsouris C, Kokolis N, Kouskoura T (1989): The effects of intrafollicular injections of plasmin and $\varepsilon$-aminocaproic acid on the ovulation in the ewe. Fibrinolysis 3:27-230
  36. Sutton R, Nancarrow CD, Wallace ALC, Rigby NW (1984): Identification of an oestrus-associated glycoprotein in oviducal fluid of the sheep. Journal of Reproduction and Fertility 72:415-422 https://doi.org/10.1530/jrf.0.0720415
  37. Taitzoglou IA, Chapman DA, Killian GJ (2003): Induction of the acrosome reaction in bull spermatozoa with plasmin. Andrologia 35:112-116 https://doi.org/10.1046/j.1439-0272.2003.00541.x
  38. Taitzoglou IA, Chapman DA, Zervos IA, Killian GJ (2004): Effect of plasmin on movement characteristics of ejaculated bull spermatozoa. Theriogenology 62:553-561 https://doi.org/10.1016/j.theriogenology.2003.11.016
  39. Thorsen S, Philipis M (1984): Isolation of tissueype plasminogen activator-inhibitor complexes from human plasma. Evidence for a rapid plasminogen activator inhibitor. Biochimica et Biophysica Acta - General Subjects 802:111-118 https://doi.org/10.1016/0304-4165(84)90040-0
  40. Tsantarliotou M (1997): The Effect of Glucocorticoids and Melatonin on the Activity of Specialized Proteases in Spermatozoa and Seminal Plasma of the Ram. Thessaloniki
  41. Vassalli JD, Sappino AP, Belin D (1991): The plasminogen activator/plasmin system. Journal of Clinical Investigation 88:1067-1072 https://doi.org/10.1172/JCI115405
  42. Verhage HG, Jaffe RC (1986): Regulation of estradiol and progesterone receptor concentrations in cat uteri following chronic progesterone administration. Journal of Steroid Biochem 24:587-590 https://doi.org/10.1016/0022-4731(86)90124-X
  43. Wagner OF, Binder BR (1986): Purification of an active plasminogen activator inhibitor immunologically related to the endothelial type plasminogen activator inhibitor from the conditioned media of a human melanoma cell line. Journal of Biological Chemistry 261:14474-14481
  44. Wijayagunawardane MPB, Cerbito WA, Miyamoto A, Acosta TJ, Takagi M, Miyazawa K, Sato K (1996): Oviductal progesterone concentration and its spatial distribution in cyclic and early pregnant cows. Theriogenology 46:1149-1158 https://doi.org/10.1016/S0093-691X(96)00286-5
  45. Wijayagunawardane MPB, Miyamoto A, Cerbito WA, Acosta TJ, Takagi M, Sato K (1998): Local distributions of oviductal estradiol, progesterone, prostaglandins, oxytocin and endothelin-1 in the cyclic cow. Theriogenology 49:607-618 https://doi.org/10.1016/S0093-691X(98)00011-9
  46. Yang NS, Park C, Longley C, Furmanski P (1983); Effect of the extracellular matrix on plasminogen activator isozyme activities of human mammary epithelial cells in culture. Mol Biol Cell, 3:982-990 https://doi.org/10.1128/MCB.3.6.982
  47. Zhang Z, Paria BC, Davis DL (1991): Pig endometrial cells in primary culture: Morphology, secretion of prostaglandins and proteins, and effects of pregnancy. Journal of Animal Science 69:3005-3015 https://doi.org/10.2527/1991.6973005x