Reproductive and Developmental Biology

  • 제32권2호
  • /
  • Pages.81-87
  • /
  • 2008
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Optimal Condition for Sperm-mediated Gene Transfer by Liposome in Pigs

  • Kim, Tae-Shin (College of Animal Life Sciences, Kangwon National University) ;
  • Yang, Cao (College of Bioengineering, Dalian University) ;
  • Lee, Young-Seung (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Soo-Bong (National Institute of Animal Science) ;
  • Park, Chun-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Dong-Seok (College of Natural Sciences, Kyungpook National University)
  • 발행 : 2008.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Production of transgenic animals for studying specific gene has been limited due to a low efficiency, lack of skilled researchers and the need for expensive equipment. Currently, the boar spermatozoa as a vector to deliver exogenous DNA into the oocyte were used to improve the efficiency of transfection rate. In this study, we revealed that the optimal conditions for DNA uptake in spermatozoa by liposome were to 90 min of incubation, $17^{\circ}C$, $10^5$ spermatozoa, 4 ng/ml of exogenous DNA and 0.5% (v/v) liposome, without damage to fertility. In addition, the developmental rate to the blastocyst stage of embryo in control group was significantly higher than those embryos with exogenous DNA and liposome, whereas there were no significant differences in embryo development between the liposome and type of DNA. The transfection rates of embryo using treated spermatozoa with both liposome and circular DNA were higher than those using linear DNA. These findings raise the possibility thattreated spermatozoa with liposome/DNA complexes could be used in in vitro fertilization, and the exogenous DNA transferred into the oocytes. Taken together, we demonstrated that liposome a vector for the uptake of exogenous DNA in boar spermatozoa could improve the efficiency of sperm-mediated gene transfer in creating transgenic pig and the other domestic transgenic animals.

키워드

참고문헌

  1. Arezzo F (1989): Sea urchin sperm as a vector of foreign genetic information. Cell Biol Int Rep 13: 391-404 https://doi.org/10.1016/0309-1651(89)90166-5
  2. Bachiller D, Schellander K, Peli J, Ruther U (1991): Liposome-mediated DNA uptake by sperm cells. Mol Reprod Dev 30:194-200 https://doi.org/10.1002/mrd.1080300305
  3. Brackett BG, Baranska W, Sawicki W, Koprowski H (1971): Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc Natl Acad Sci USA 68:353-357
  4. Carballada R, Degefa T, Esponda P (2000): Transfection of mouse eggs and embryos using DNA combined to cationic liposomes. Mol Reprod Dev 56: 360-365 https://doi.org/10.1002/1098-2795(200007)56:3<360::AID-MRD5>3.0.CO;2-8
  5. Chancham P, Hughes JA (2001): Relationship between plasmid DNA topological forms and in vitro transfection. J Liposome Res 11:139-152 https://doi.org/10.1081/LPR-100108458
  6. Chen ZY, Yant SR, He CY, Meuse L, Shen S, Kay MA (2001): Linear DNAs concatemerize in vivo and result in sustained transgene expression in mouse liver. Molecular Therapy 3:403-410 https://doi.org/10.1006/mthe.2001.0278
  7. Felgner PL, Tsai YJ, Sukhu L, Wheeler CJ, Manthorpe M, Marshall J, Cheng SH (1995): Improved cationic lipid formulations for in vivo gene therapy. Ann N Y Acad Sci 772:126-139 https://doi.org/10.1111/j.1749-6632.1995.tb44738.x
  8. Hogan B, Beddington R, Costantini F, Lacy E (1994): Manipulating the Mouse Embryo: A Laboratory Manual
  9. Khoo HW, Ang LH, Lim HB, Wong KY (1992): Sperm cells as vectors for introducing foreign DNA into zebrafish. Aquaculture 107:1-19 https://doi.org/10.1016/0044-8486(92)90046-N
  10. Lavitrano M, French D, Zani M, Frati L, Spadafora C (1992): The interaction between exogenous DNA and sperm cells. Mol Reprod Dev 31:161-169 https://doi.org/10.1002/mrd.1080310302
  11. Lavitrano M, Maione B, Forte E, Francolini M, Sperandio S, Testi R, Spadafora C (1997): The interaction of sperm cells with exogenous DNA: A role of CD4 and major histocompatibility complex class II molecules. Exp Cell Res 233:56-62 https://doi.org/10.1006/excr.1997.3534
  12. Liu Y, Liggitt D, Zhong W, Tu G, Gaensler K, Debs R (1995): Cationic liposome-mediated intravenous gene delivery. J Biol Chem 270:24864-24870 https://doi.org/10.1074/jbc.270.42.24864
  13. Perez A, Solano R, Castro R, Lleonart R, De Armas R, Martinez R, Aguilar A, Herrera L, De la Fuente J (1991): Sperm cells mediated gene transfer in cattle. Biotechnol Aplicada 8:90-94
  14. Rottmann O, Antes R, Hofer P (1996): Liposomemediated gene transfer via sperm cells. High transfer efficiency and persistence of transgenes by use of liposomes and sperm cells and a murine amplification element. J Anim Breed Genet 113: 401-411 https://doi.org/10.1111/j.1439-0388.1996.tb00631.x
  15. Rottmann OJ, Antes R, Hoefer P, Maierhofer G (1992): Liposome mediated gene transfer via spermatozoa into avian egg cells. J Anim Breed Genet 109:64-70 https://doi.org/10.1111/j.1439-0388.1992.tb00379.x
  16. Shemesh M, Gurevich M, Harel-Markowitz E, Benvenisti L, Shore LS, Stram Y (2000): Gene integration into bovine sperm genome and its expression in transgenic offspring. Mol Reprod Dev 56:306- 308 https://doi.org/10.1002/(SICI)1098-2795(200006)56:2+<306::AID-MRD21>3.0.CO;2-3
  17. Yoshioka K, Suzuki C, Tanaka A, Anas IMK, Iwamura S (2002): Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod 66:112-119 https://doi.org/10.1095/biolreprod66.1.112
  18. Zani M, Lavitrano M, French D, Lulli V, Maione B, Sperandio S, Spadafora C (1995): The mechanism of binding of exogenous DNA to sperm cells: Factors controlling the DNA uptake. Exp Cell Res 217:57-64 https://doi.org/10.1006/excr.1995.1063