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Plasminogen Activators Activities in Oviductal Epithelial Cells during Estrus Cycle in the Pig  

Shin, Mi-Young (College of Animal Life Sciences, Kangwon National University)
Kim, Tae-Shin (College of Animal Life Sciences, Kangwon National University)
Kwon, Eun-Hye (Korea Basic Science Institute Chuncheon Center, Kangwon National University)
Park, Soo-Bong (National Institute of Animal Science)
Park, Chun-Keun (College of Animal Life Sciences, Kangwon National University)
Lee, Dong-Seok (College of Natural Sciences, Kyungpook National University)
Publication Information
Reproductive and Developmental Biology / v.32, no.2, 2008 , pp. 89-95 More about this Journal
Abstract
The present study was undertaken to identify changes of plasminogen activators (PAs) in porcine oviductal epithelial cells (POECs) during the estrous cycle classified with post-ovulatory stages (Post-Ov), early to mid-luteal stages (Early-mid L) and pre-ovulatory (Pre-Ov) stages. The urokinase-type plasminogen activator (uPA) was only observed on day 5 and day 7 of culture in the POECs on all the estrous cycles and gradually increased according to increasing culture times, but not Early-mid L. In POECs-conditioned medium, uPA, tissue-type (tPA) and tPA-PA inhibitor (tPA-PAI) activity were observed at all culture times during estrous cycles. The uPA activity of POECs-conditioned medium on Post-Ov stage were significantly (p<0.05) decreased during prolonged cultures. On the other hand, the tPA activity of POECs-conditioned medium at Post-Ov stage was significantly (p<0.05) higher on day 5 than compared to the other days. Although was higher on day 1 at Post-Ov stage, the tPA-PAI activity of POECs-conditioned medium was significantly (p<0.05) higher on day 7 at all stage than that of day 5 of the culture. Taken together, these results suggest that uPA, tPA and tPA-PAI are produced by POECs, and the variations of the PAs activity are regulated in the different stages of the estrous cycle.
Keywords
Plasminogen activators; Oviduct; Estrous cycle; Epithelial cells; Pig;
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