Effect of Foliar Uptake of Azoxystrobin and Kresoxim-methyl on Fungicidal Activity against Cucumber Powdery Mildew

Azoxystrobin과 Kresoxim-methyl의 오이 엽면 침투성과 오이 흰가루병 방제 효과

  • Yu, Ju-Hyun (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung-Ja (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Heung-Tae (Department of Plant Medicine, Chungbuk National University)
  • 유주현 (한국화학연구원 바이오정밀화학연구센터) ;
  • 최경자 (한국화학연구원 바이오정밀화학연구센터) ;
  • 김흥태 (충북대학교 식물의학과)
  • Published : 2008.06.30

Abstract

Azoxystrobin applied by aqueous WP suspension 50 mg/l was slightly absorbed into cucumber leaf in the absence of activator surfactant 24 h after spraying, but was increased to 25.7% by adding polyoxyethylene monohexadecyl ether (12 moles of ethylene oxide, CE-12) 500 mg/l. Only 4.1% of kresoxim-methyl WDG 100 mg/l in the absence of surfactant was absorbed into cucumber leaf 24 h after spraying, but was increased to 58.0% by adding polyoxyethylene monooctadecyl ether (14 moles of ethylene oxide, SE-14) 1,000 mg/l. The effect of CE-12 500 mg/l on foliar uptake of kresoxim-methyl at 50 mg/l was twice bigger than on azoxystrobin. Fungicidal activity of azoxystrobin WP against cucumber powdery mildew was marginally increased by adding surfactants to facilitate foliar uptake of azoxystrobin, so that the further increase of azoxystrobin uptake into cucumber plant by the addition of adjuvant was not a practical mean for enhancing the formulation efficacy in view of fungicidal activity. It was not possible for kresoxim-methyl to assess the adjuvant effect on the fungicidal activity in a greenhouse trial due to the vapor effect of active ingredient.

Azoxystrobin과 kresoxim-methyl의 오이 흰가루병 방제 효과를 증진시키기 위하여 오이 잎에서 유효성분의 침투성을 증진시킬 수 있는 계면활성제를 선발하고 이를 함유하는 제제의 오이 흰가루병에 대한 방제효과를 측정하였다. 수화제 현탁액으로 살포한 azoxystrobin은 오이 잎에 거의 침투되지 않았으나 polyoxyethylene monohexadecyl ether(CE-12, 에틸렌옥사이드 12몰 중합물)를 500 mg/l 첨가함으로써 침투성이 분무 24시간 후에 25.7%까지 증가하였다. 수화성 입제를 물에 희석하여 100 mg/l의 농도로 처리한 kresoxim-methyl은 4.1%만이 오이잎에 침투되었지만 polyoxyethylene monooctadecyl ether(에틸렌옥사이드 14몰 중합물)를 1,000 mg/l 첨가하였을 때 침투율이 58.0%까지 증가하였따. 계면활성제 CE-12(500 mg/l)에 의한 오이 엽면 침투율은 같은 유효성분 농도(50 mg/l)에서 kresoximmethyl이 azoxystrobin보다 2배 이상 높았다. 침투성 증진용 계면활성제를 첨가한 azoxystrobin 수화제 현탁액은 수화제 처리 대조구보다 온실 내 오이 흰가루병 방제 효과를 증가시켰지만 그 차이는 크지 않았다. 그러므로 azoxystrobin의 경우에는 침투성 증진이 오이 흰가루병에 대한 방제 효과를 크게 높일 수 있는 실용적인 방법은 아니었다. 반면에 kresoxim-methyl은 유효성분의 높은 증기압 때문에 동절기 말폐된 온실 내에서는 침투성 증진에 따른 오이 흰가루병 방제 효과 변화를 측정할 수 없었다.

Keywords

References

  1. Tomlin, C. D. S. (2000) In 'The Pesticide Manual' Twelfth Ed., British Crop Protection Council, Surrey, UK
  2. Dave, W. B., John, M. C., Jeremy, G. G., Alison, A. H., Mick, H. and Bob, P-D. (2002) The strobilurin. Pest Manag. Sci. 58, 649-662 https://doi.org/10.1002/ps.520
  3. Ypema, H. L. and Gold R. E. (1999) Kresoxim-methylmodification of a naturally occurring compound to produce a new fungicide. Plant Disease 83, 4-19 https://doi.org/10.1094/PDIS.1999.83.1.4
  4. Godwin, J. R., Young, J. E. and Hart, C. E. (1994) ICIA5504: Effects on development of cereal pathogens, in Proc Brighton Crop Protect Conf-Pests and Diseases, BCPC, Farnham, Surrey, UK, pp 259-264
  5. Leinhos, G. M. E., Gold R. E., Duggelin, M. and Guggenheim, R. (1997) Development and morphology of Uncinula necator following treatment with the fungicides kresoxim-methyl and penconazole. Mycol. Res. 101, 1033-1046 https://doi.org/10.1017/S0953756297003651
  6. Wong, F. P. and Wilcox, W. F. (2001) Comparative physical modes of action of azoxystrobin, mancozeb, and metalaxyl against Plasmopara viticola. Plant Disease 85, 649-656 https://doi.org/10.1094/PDIS.2001.85.6.649
  7. Cho, K. Y., Yu, J. H., Lim, H. K., Choi, G. J. and Kim, J. H. (1999) Composition and method for measuring the foliar uptake of agrochemicals. Patent Application PCT/KR 99/00342
  8. Yu, J. H., Lim, H. K., Choi, G. J., Cho, K. Y. and Kim, J. H. (2001) A new method for assessing foliar uptake of fungicides using Congo Red as a tracer. Pest Manag. Sci. 57, 564-569 https://doi.org/10.1002/ps.327
  9. Stock, D., Holloway, P. J. (1993) Possible mechanisms for surfactant-induced foliar uptake of agrochemicals. Pestic. Sci. 38, 165-177 https://doi.org/10.1002/ps.2780380211
  10. Bauer, P., Grayson, B. T. and Schonherr, J. (1997) Polydispersed ethoxylated fatty alcohol surfactants as accelerators of cuticular penetration. 1. Effect of ethoxy chain length and size of penetrants. Pestic. Sci. 51, 131-152 https://doi.org/10.1002/(SICI)1096-9063(199710)51:2<131::AID-PS614>3.0.CO;2-D
  11. Yu, J. H. (2000) Evaluation of Foliar Uptake of Pesticide: Method Development and Application, Ph.D. Thesis, Seoul National University, School of Agricultural Biotechnology, Suwon, Korea