Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Butein-Induced Apoptosis in Human T Lymphoma Jurkat Cells

Butein의 Jurkat T 림포마 세포에서 발현되는 세포괴사 효과

  • Kim, Na-young (Children's Hospital Boston, Department of Medicine, and Harvard Medical School, Department of Pediatrics)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Butein is a one of polyphenolic compound widely available in numerous plants. It has broad biological activities including antioxidant and anti-inflammatory activities, which contributed to its protective effects against cancer. Evidences that butein influence proliferation of tumor cells make it important to determine how butein affects cell death of various cancers. In this study, we show that butein, a phenolic compound, induces apoptosis in human T lymphoma jurkat cells. We found that treatment of cells with butein increased apoptosis in a dose- and time- dependent manner as determined by staining cells with Annexin V and 7AAD. There was no significant apoptotic cell death when normal lymphocytes and monocytes from healthy donor were treated with butein. We also found caspase-3 activity was increased during butein-induced apoptosis. The buteininduced apoptotic cell death was blocked by the treatment of cells with caspase-3 inhibitor. These results indicate that butein has the potential to provide an effective strategy against cancer with the advantage of being widely avalible.

Keywords

References

  1. Bravo, L. (1998) Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56: 317-333 https://doi.org/10.1111/j.1753-4887.1998.tb01670.x
  2. Manach, C., Scalbert, A., Morand, C., Rémésy, C. and Jiménez, L. (2004) Polyphenols: food sources and bioavailability. Am. J Clin. Nutr. 79: 727-747 https://doi.org/10.1093/ajcn/79.5.727
  3. Rice-Evans, C. A., Miller, N. J., Bolwell, P. G., Bramley, P. M. and Pridham, J. B. (1995) The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res. 22: 375-383 https://doi.org/10.3109/10715769509145649
  4. Bergamaschi, G. V., Rosti, M., Danova, L., Ponchio, C. and Lucotti, M. (1993) Cazzola: Inhibitors of tyrosine phosphorylation induce apoptosis in human leukaemic cell lines. Leukemia 7: 2012-2018
  5. Constantinou, A. I., Kamath, N. and Murley, J. S. (1998) Genistein inactivates bcl-2, delays the G2/M phase of the cell cycle, and induces apoptosis of human breast adenocarcinoma MCF-7 cells. Eur. J. Cancer. 34: 1927-1934 https://doi.org/10.1016/S0959-8049(98)00198-1
  6. Csokay, B., Prajda, N., Weber, G. and Olah, E. (1997) Molecular mechanisms in the antiproliferative action of quercetin. Life Sci. 60: 2157-2163 https://doi.org/10.1016/S0024-3205(97)00230-0
  7. Fioravanti, L., Cappelletti, V., Miodini, P., Ronchi, E., Brivio, M. and Di Fronzo, D. (1998) Geninstein in the control of breast cancer cell growth: insights into the mechanism of action in vitro. Cancer Lett. 130: 143-152 https://doi.org/10.1016/S0304-3835(98)00130-X
  8. Pagliacci, M. C., Smacchia, M., Migliorati, G., Grignani, F., Riccardi, C. and Nicoletti, I. (1994) Growth-inhibitory effects of the natural phyto-genistein in MCF-7 human breast cancer cells. Eur. J. Cancer 11: 1675-1682
  9. Shao, Z. M., Wu, J., Shen, Z.Z. and Barsky, S. H. (1998) Genistein inhibits both constitutive and EGF-stimulated invasion in ER-negative human breast carcinoma cell lines Anticancer Res. 18: 1435-1439
  10. Kang, T. B. and Liang, N. C. (1997) Studies on the inhibitory effects of quercetin on the growth of HL-60 leukemia cells. Biochem. Pharmacol. 54: 1013-1018 https://doi.org/10.1016/S0006-2952(97)00260-8
  11. Lepley, D. M. and Pelling, J. C. (1997) Induction of p21/ WAF1 and G1 cell cycle arrest by the chemopreventive agent apigenin. Mol. Carcinogen 19: 74-82 https://doi.org/10.1002/(SICI)1098-2744(199707)19:2<74::AID-MC2>3.0.CO;2-L
  12. Bellosillo, B., Pique, M., Barragan, M., Castano, E., Villamor, N., Colomer, D., Montserrat, E., Pons, G. and Gil, J. (1998) Aspirin and salicylate induce apoptosis and activation of caspase in B-cell chronic lymphocytic leukaemia cells. Blood 92: 1406-1414
  13. Samaha, H. S., Kelloff, G. J., Steele, V., Rao, C. V. and Reddy, B. S. (1997) Modulation of apoptosis by sulindac, curcumin, phenyl-3-methylcaffeate, and 6-phenylhexyl isothiocyanate: apoptotic index as a biomarker in colon cancer chemoprevention and promotion. Cancer Res. 57: 1301-1305
  14. Budihardjo, I., H., Oliver, M., Lutter, X. and Wang, X. (1999) Biochemical pathways of caspase activation during apoptosis. Annu. Rev. Cell Dev. Biol. 15: 269-290 https://doi.org/10.1146/annurev.cellbio.15.1.269
  15. Danial, N. N. and Korsmeyer, S. J. (2004) Cell death: critical control points. Cell 116: 205-219 https://doi.org/10.1016/S0092-8674(04)00046-7
  16. Earnshaw, W. C., Martins, L. M. and Kaufmann, S. H. (1999) Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem. 68: 383-424 https://doi.org/10.1146/annurev.biochem.68.1.383
  17. Strasser, A., O'Connor, L. and Dixit, V. M. (2000) Apoptosis signaling. Annu. Rev. Biochem. 69: 217-245 https://doi.org/10.1146/annurev.biochem.69.1.217
  18. Kim, N. Y., Pae, H. O., Oh, G. S., Kang, T. H., Kim, Y. C., Rhew, H. Y. and Chung, H. T. (2001) Pharmacol. Toxicol. 88: 261-266 https://doi.org/10.1034/j.1600-0773.2001.d01-114.x
  19. Ramanathan, R., Tan, C. H. and Das, N .P. (1992) Cytotoxic effects of plant polyphenols and fat-soluble vitamins on malignant human cultured cells. Cancer Lett. 62: 217-214 https://doi.org/10.1016/0304-3835(92)90099-H
  20. Yit, C. C. and Das, N. P. (1994) Cytotoxic effects of butein on human colon adenocarcinoma cell proliferation. Cancer Lett. 82: 65-72 https://doi.org/10.1016/0304-3835(94)90147-3
  21. Chan, S. C., Chang, Y. S., Wang, J. P., Chen, S. C. and Kuo, S. C. (1998) Three new flavonoids and antiallergic, antiinflammatory constituents from heartwood of Dalbergia odorifera. Planta Med. 64: 153-158 https://doi.org/10.1055/s-2006-957394
  22. Cheng, Z. Y., Kuo, S. C., Chan, S. C., Ko, F. N. and Teng, C. M. (1998) Antioxidant properties of butein isolated from Dalbergia odorifera. Biochim. Biophys. Acta. 1392: 291-299 https://doi.org/10.1016/S0005-2760(98)00043-5
  23. Wang, Y., Chan, F. L., Chen, S. and Leung, L. K. (2005) The plant polyphenol butein inhibits testosterone-induced proliferation in breast cancer cells expressing aromatase. Life Sci. 77: 39-51 https://doi.org/10.1016/j.lfs.2004.12.014
  24. Samoszuk, M., Tan, J. and Chorn, G. (2005) The chalcone butein from Rhus verniciflua Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts. BMC Complement Altern. Med. 5: 1-5 https://doi.org/10.1186/1472-6882-5-1
  25. Kang, H. M., Kim, J. H., Lee, M. Y., Son, K. H., Yang, D. C., Baek, N. I. and Kwon, B. M. (2004) Relationship between flavonoid structure and inhibition of farnesyl protein transferase. Nat. Prod. Res. 18: 349-356 https://doi.org/10.1080/14786410310001622022
  26. Jang, H. S., Kook, S. H., Son, Y. O., Kim, J. G., Jeon, Y. M., Jang, Y. S., Choi, K. C., Kim, J., Han, S. K., Lee, K. Y., Park, B. K., Cho, N. P. and Lee, J. C. (2005) Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells. Biochim. Biophys. Acta. 1726: 309-316 https://doi.org/10.1016/j.bbagen.2005.08.010
  27. Lee, J. C., Lee, K. Y., Kim, J., Na, C. S., Jung, N. C., Chung, G. H. and Jang, Y. S. (2004) Extract from Rhus verniciflua Stokes is capable of inhibiting the growth of human lymphoma cells. Food Chem. Toxicol. 42: 1383-1388 https://doi.org/10.1016/j.fct.2004.03.012
  28. Iwashita, K., Kobori, M., Yamaki, K. and Tsushida, T. (2000) Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells. Biosci. Biotechnol. Biochem. 64: 1813-1820 https://doi.org/10.1271/bbb.64.1813
  29. Lee, S. H., Seo, G. S., Kim, H. S., Woo, S. W., Ko, G. and Sohn, D. H. (2006) 2',4',6'-Tris(methoxymethoxy) chalcone attenuates hepatic stellate cell proliferation by a heme oxygenase- dependent pathway. Biochem. Pharmacol. 72: 1322-1333 https://doi.org/10.1016/j.bcp.2006.08.004