Browse > Article

Butein-Induced Apoptosis in Human T Lymphoma Jurkat Cells  

Kim, Na-young (Children's Hospital Boston, Department of Medicine, and Harvard Medical School, Department of Pediatrics)
Publication Information
Korean Journal of Pharmacognosy / v.39, no.2, 2008 , pp. 150-154 More about this Journal
Abstract
Butein is a one of polyphenolic compound widely available in numerous plants. It has broad biological activities including antioxidant and anti-inflammatory activities, which contributed to its protective effects against cancer. Evidences that butein influence proliferation of tumor cells make it important to determine how butein affects cell death of various cancers. In this study, we show that butein, a phenolic compound, induces apoptosis in human T lymphoma jurkat cells. We found that treatment of cells with butein increased apoptosis in a dose- and time- dependent manner as determined by staining cells with Annexin V and 7AAD. There was no significant apoptotic cell death when normal lymphocytes and monocytes from healthy donor were treated with butein. We also found caspase-3 activity was increased during butein-induced apoptosis. The buteininduced apoptotic cell death was blocked by the treatment of cells with caspase-3 inhibitor. These results indicate that butein has the potential to provide an effective strategy against cancer with the advantage of being widely avalible.
Keywords
Butein; Apoptosis; Anti-oxidant; Polyphenol;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Bergamaschi, G. V., Rosti, M., Danova, L., Ponchio, C. and Lucotti, M. (1993) Cazzola: Inhibitors of tyrosine phosphorylation induce apoptosis in human leukaemic cell lines. Leukemia 7: 2012-2018
2 Csokay, B., Prajda, N., Weber, G. and Olah, E. (1997) Molecular mechanisms in the antiproliferative action of quercetin. Life Sci. 60: 2157-2163   DOI   ScienceOn
3 Shao, Z. M., Wu, J., Shen, Z.Z. and Barsky, S. H. (1998) Genistein inhibits both constitutive and EGF-stimulated invasion in ER-negative human breast carcinoma cell lines Anticancer Res. 18: 1435-1439
4 Danial, N. N. and Korsmeyer, S. J. (2004) Cell death: critical control points. Cell 116: 205-219   DOI   ScienceOn
5 Kim, N. Y., Pae, H. O., Oh, G. S., Kang, T. H., Kim, Y. C., Rhew, H. Y. and Chung, H. T. (2001) Pharmacol. Toxicol. 88: 261-266   DOI   ScienceOn
6 Ramanathan, R., Tan, C. H. and Das, N .P. (1992) Cytotoxic effects of plant polyphenols and fat-soluble vitamins on malignant human cultured cells. Cancer Lett. 62: 217-214   DOI   ScienceOn
7 Yit, C. C. and Das, N. P. (1994) Cytotoxic effects of butein on human colon adenocarcinoma cell proliferation. Cancer Lett. 82: 65-72   DOI   ScienceOn
8 Wang, Y., Chan, F. L., Chen, S. and Leung, L. K. (2005) The plant polyphenol butein inhibits testosterone-induced proliferation in breast cancer cells expressing aromatase. Life Sci. 77: 39-51   DOI   ScienceOn
9 Kang, H. M., Kim, J. H., Lee, M. Y., Son, K. H., Yang, D. C., Baek, N. I. and Kwon, B. M. (2004) Relationship between flavonoid structure and inhibition of farnesyl protein transferase. Nat. Prod. Res. 18: 349-356   DOI   ScienceOn
10 Rice-Evans, C. A., Miller, N. J., Bolwell, P. G., Bramley, P. M. and Pridham, J. B. (1995) The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res. 22: 375-383   DOI
11 Iwashita, K., Kobori, M., Yamaki, K. and Tsushida, T. (2000) Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells. Biosci. Biotechnol. Biochem. 64: 1813-1820   DOI   ScienceOn
12 Bravo, L. (1998) Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56: 317-333   DOI   ScienceOn
13 Samoszuk, M., Tan, J. and Chorn, G. (2005) The chalcone butein from Rhus verniciflua Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts. BMC Complement Altern. Med. 5: 1-5   DOI   ScienceOn
14 Lee, J. C., Lee, K. Y., Kim, J., Na, C. S., Jung, N. C., Chung, G. H. and Jang, Y. S. (2004) Extract from Rhus verniciflua Stokes is capable of inhibiting the growth of human lymphoma cells. Food Chem. Toxicol. 42: 1383-1388   DOI   ScienceOn
15 Jang, H. S., Kook, S. H., Son, Y. O., Kim, J. G., Jeon, Y. M., Jang, Y. S., Choi, K. C., Kim, J., Han, S. K., Lee, K. Y., Park, B. K., Cho, N. P. and Lee, J. C. (2005) Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells. Biochim. Biophys. Acta. 1726: 309-316   DOI   ScienceOn
16 Lepley, D. M. and Pelling, J. C. (1997) Induction of p21/ WAF1 and G1 cell cycle arrest by the chemopreventive agent apigenin. Mol. Carcinogen 19: 74-82   DOI
17 Cheng, Z. Y., Kuo, S. C., Chan, S. C., Ko, F. N. and Teng, C. M. (1998) Antioxidant properties of butein isolated from Dalbergia odorifera. Biochim. Biophys. Acta. 1392: 291-299   DOI   ScienceOn
18 Fioravanti, L., Cappelletti, V., Miodini, P., Ronchi, E., Brivio, M. and Di Fronzo, D. (1998) Geninstein in the control of breast cancer cell growth: insights into the mechanism of action in vitro. Cancer Lett. 130: 143-152   DOI   ScienceOn
19 Bellosillo, B., Pique, M., Barragan, M., Castano, E., Villamor, N., Colomer, D., Montserrat, E., Pons, G. and Gil, J. (1998) Aspirin and salicylate induce apoptosis and activation of caspase in B-cell chronic lymphocytic leukaemia cells. Blood 92: 1406-1414
20 Strasser, A., O'Connor, L. and Dixit, V. M. (2000) Apoptosis signaling. Annu. Rev. Biochem. 69: 217-245   DOI   ScienceOn
21 Budihardjo, I., H., Oliver, M., Lutter, X. and Wang, X. (1999) Biochemical pathways of caspase activation during apoptosis. Annu. Rev. Cell Dev. Biol. 15: 269-290   DOI   ScienceOn
22 Kang, T. B. and Liang, N. C. (1997) Studies on the inhibitory effects of quercetin on the growth of HL-60 leukemia cells. Biochem. Pharmacol. 54: 1013-1018   DOI   ScienceOn
23 Samaha, H. S., Kelloff, G. J., Steele, V., Rao, C. V. and Reddy, B. S. (1997) Modulation of apoptosis by sulindac, curcumin, phenyl-3-methylcaffeate, and 6-phenylhexyl isothiocyanate: apoptotic index as a biomarker in colon cancer chemoprevention and promotion. Cancer Res. 57: 1301-1305
24 Earnshaw, W. C., Martins, L. M. and Kaufmann, S. H. (1999) Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem. 68: 383-424   DOI   ScienceOn
25 Pagliacci, M. C., Smacchia, M., Migliorati, G., Grignani, F., Riccardi, C. and Nicoletti, I. (1994) Growth-inhibitory effects of the natural phyto-genistein in MCF-7 human breast cancer cells. Eur. J. Cancer 11: 1675-1682
26 Chan, S. C., Chang, Y. S., Wang, J. P., Chen, S. C. and Kuo, S. C. (1998) Three new flavonoids and antiallergic, antiinflammatory constituents from heartwood of Dalbergia odorifera. Planta Med. 64: 153-158   DOI   ScienceOn
27 Manach, C., Scalbert, A., Morand, C., Rémésy, C. and Jiménez, L. (2004) Polyphenols: food sources and bioavailability. Am. J Clin. Nutr. 79: 727-747   DOI
28 Lee, S. H., Seo, G. S., Kim, H. S., Woo, S. W., Ko, G. and Sohn, D. H. (2006) 2',4',6'-Tris(methoxymethoxy) chalcone attenuates hepatic stellate cell proliferation by a heme oxygenase- dependent pathway. Biochem. Pharmacol. 72: 1322-1333   DOI   ScienceOn
29 Constantinou, A. I., Kamath, N. and Murley, J. S. (1998) Genistein inactivates bcl-2, delays the G2/M phase of the cell cycle, and induces apoptosis of human breast adenocarcinoma MCF-7 cells. Eur. J. Cancer. 34: 1927-1934   DOI   ScienceOn