Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Responses of Alkoxyresorufin Dealkylases and Glutathione S-transferase Activities of Surf Clam, Pseudocardium sachalinensis, Injected with Sea-Nine 211 Antifoulant

Tin-free 방오제인 Sea-Nine 211을 주사한 북방대합에서 alkoxyresorufin 탈알킬화효소와 글루타치온 포합효소 활성의 변화

  • 이지선 (강릉대학교 해양생명공학부.동해안해양생물자원연구센터(EMBRC)) ;
  • 전영하 (강원도 환동해출장소) ;
  • 심원준 (한국해양연구원 남해연구소) ;
  • 전중균 (강릉대학교 해양생명공학부.동해안해양생물자원연구센터(EMBRC))
  • Published : 2008.05.31
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Abstract

To evaluate the extent of Sea-Nine 211 (4,5-dichloro-2-n-octyl-3(2H) isothiazolone), one of the alternating organic booster compound used in antifouling paint to replace TBT, on marine benthic bivalve, we injected Sea-Nine 211 to clam, Pseudocardium sachalinensis, and then determined some xenobiotics metabolizing enzyme activities, especially EROD (ethoxyresorufin deethylase) and MROD (methoxyresorufin demethylase), in digestive gland during 4 day-exposure period. Moreover, the results were compared with those of TBT exposed clam. CYP1A1 dependant EROD activity in both the Sea-Nine 211 and the TBTC exposure groups showed no significant differences compared to those of the solvent control group. CYP1A2 dependant MROD activity in Sea-Nine 211 exposure group was significantly induced, but no significant difference was obtained in the TBTC exposure group. These results indicate that Sea-Nine 211 demonstrated a tendency to induce MROD activity, while TBTC inhibits the activities of this enzyme.

방오도료로 많이 쓰이던 유기주석화합물은 일반생물에게 미치는 독성이 매우 강하고 또한 내분비계 장애물질임이 밝혀지면서 이를 대체할 화합물들이 개발되고 있다. 그 가운데 본 연구에서는 Sea-Nine 211(4,5-dichlore-2-n-octyl-3(2H) isothiazolone)을 사용하여 이 화합물이 해양생물 특히 저서생물인 패류에게 얼마나 영향을 미치는지를 살펴보았다. 이를 위해 강원도 북부 해역에 주로 서식하는 북방대합(Pseudocardium sachalinensis)에게 Sea-Nine 211을 강제적으로 주사하여 alkoxyresorufin 탈알킬화효소인 EROD와 MROD의 활성 변화를 4일째까지 조사하였고, 비교를 위해서 tributyltin chloride (TBTC)도 사용하였다. 그 결과, CYP1A1의 지표효소인 EROD활성은 Sea-Nine 211 및 TBTC주사구 모두 sham구와 차이가 없었지만, CYP1A2의 지표효소인 MROD활성은 Sea-Nine 211 주사구가 sham구에 비해 유의적으로 높았으므로 Sea-Nine 211에 의해 유도된 것이라 여겨진다. 이것은 북방대합에도 CYP1A2가 존재할 수 있음을 보여준다.

Keywords

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