Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

The Cytotoxic Action of New Ag-Porphyrin as a Potential Chemotherapeutic Agent

  • Nelli, Babayan (Department of Genetics and Cytology, Yerevan State University) ;
  • Artak, Tovmasyan (Department of General and Organic Chemistry, Yerevan State Medical University) ;
  • Ani, Gevorkyan (Department of Genetics and Cytology, Yerevan State University) ;
  • Gennadi, Gasparyan (Department of Genetics and Cytology, Yerevan State University) ;
  • Rouben, Aroutiounian (Department of Genetics and Cytology, Yerevan State University)
  • Published : 2008.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Earlier we have described new water-soluble Ag- and Zn-derivatives of tetrachloride meso-tetra (4-N-oxiethylpyridyl) porphyrin (TOEtPyP) as potential anticancer drugs. In this work the effect of one of these metal porphyrins, TOEtPyP Ag, on the cell population kinetics was studied in vitro using morphological and biochemical techniques. The results suggested that TOEtPyP Ag action consisted in the simultaneous suppression of the cell growth and activation of the cell death. About 40% of the cells were shown to die via apoptotic pathway. So, the porphyrin studied may be attributed to inducers of both necrotic and apoptotic processes. The results obtained support our previous assertion that TOEtPyP Ag may be considered as a potential chemotherapeutic agent.

Keywords

References

  1. Aderem A and DM Underhill. 1999. Mechanisms of phagocytosis in macrophages. Annu. Rev. Immunol. 17:593-623 https://doi.org/10.1146/annurev.immunol.17.1.593
  2. Al-Mutairi DA, JD Craik, I Batinic-Haberle and LT Benov. 2006. Photosensitizing action of isomeric Zn N-methylpyridyl porphyrins in human carcinoma cells. Free Radical Res. 40(5):477-483 https://doi.org/10.1080/10715760600577849
  3. Berg K, PK Selbo, A Weyergang, A Dietze, L Prasmickaite, A Bonsted, BO Engesaeter, E Angell-Petersen, T Warloe, N Frandsen and A Hogset. 2005. Porphyrin-related photosensitizers for cancer imaging and therapeutic applications. J. Microsc. 218(2):133-147
  4. Cohen GM, X Sun, RT Snowden, D Dinsdale and DN Skilleter. 1992. Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochemical J. 286:331-334 https://doi.org/10.1042/bj2860331
  5. Darzynkiewicz Z. 1990. Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange. Methods Cell Biol. 33:285-98 https://doi.org/10.1016/S0091-679X(08)60532-4
  6. Ding L, G Etemad-Moghadam and B Meunier. 1990. Oxidative cleavage of DNA mediated by hybrid metalloporphyrin- ellipticine molecules and functionalized metalloporphyrin precursors. Biochemistry. 29:7868-7875 https://doi.org/10.1021/bi00486a013
  7. Ding L, J Balzarini, D Schols, B Meunier and E De Clercq. 1992. Anti-human immunodeficiency virus effects of cationic metalloporphyrin-ellipticine complexes. Biochem. Pharmacol. 44:1675-1679 https://doi.org/10.1016/0006-2952(92)90486-3
  8. Dougherty TJ, CJ Gomer, BW Henderson, G Jori, D Kessel, M Korbelik, J Moan and Q Peng. 1998. Photodynamic therapy. J. Natl. Cancer Inst. 90:889-905 https://doi.org/10.1093/jnci/90.12.889
  9. Foglieni C, C Meoni and AM Davalli. 2001. Fluorescent dyes for cell viability: an application on prefixed conditions. Histochem.Cell Biol. 115:223-229
  10. Gasparyan G, G Hovhannisyan, R Ghazaryan, L Sahakyan, A Tovmasyan, R Grigoryan, N Sarkissyan, K Margaryan, S Haroutiunian and R Arutyunyan. 2007. In vitro testing of cyto- and genotoxicity of new porphyrin water-soluble metal derivatives. Int. J. Toxicol. 26:479-502
  11. Ghazaryan RK, LA Sahakyan and AG Tovmasyan. 2006a. Patent of the Republic of Armenia No 1716 A2
  12. Ghazaryan RK, LA Sahakyan and AG Tovmasyan. 2006b. Patent of the Republic of Armenia No 1715 A2
  13. Ghazaryan RK, LA Sahakyan and AG Tovmasyan. 2006c. Patent of the Republic of Armenia No 1714 A2
  14. Hale AJ, CA Smith, LC Sutherland, VE Stoneman, V Longthorne, AC Culhane and GT Williams. 1996. Apoptosis: molecular regulation of cell death. Eur. J. Biochem. 237:884
  15. Hayhome BA. 1981. Giemsa Staining to Monitor Cell Viability in Peridinium cinctum fa. Ovoplanum. Trans. Am. Microsc. Soc. 100:410-414 https://doi.org/10.2307/3226154
  16. In Vitro Diagnostic Medical Device. 2004. Acridine orange zinc chloride double salt (C.I.46005) Certistain
  17. Jori G. 2006. Photodynamic therapy of microbial infections: state of the art and perspectives. J. Environ. Pathol. Toxicol. Oncol. 25:505-519 https://doi.org/10.1615/JEnvironPatholToxicolOncol.v25.i1-2.320
  18. Kadish KM. 2000. The Porphyrin Handbook (Smith KM and Guilard R Eds.). Academic Press. San Diego
  19. Lambrechts SA, MC Aalders, FD Verbraak, JW Lagerberg, JB Dankert and JJ Schuitmaker. 2005. Effect of albumin on the photodynamic inactivation of microorganisms by a cationic porphyrin. Photochem Photobiol. 79:51-57 https://doi.org/10.1016/j.jphotobiol.2004.11.020
  20. Luo Y, CK Chang and D Kessel. 1996. Rapid Initiation of Apoptosis by Photodynamic Therapy. Photochem. Photobiol. 63(4):528-534 https://doi.org/10.1111/j.1751-1097.1996.tb03079.x
  21. Martirosyan A, V Hovhanesyan, S Gasparyan, S Mamayan, R Ghazaryan and V Madakyan. 2006. Metal-complex catalysis during the reduction of functional groups by borohydride in the synthesis of pyrrolidine derivatives. Chem. Heterocyclic Compounds. 42(2):180-183 https://doi.org/10.1007/s10593-006-0068-4
  22. Ogura Sh, T Kamachi and I Okura. 2005. Cellular localization and photodynamic effect of chlorin e6-monoclonal antibody conjugate. J. Porphyrins Phthalocyanines. 9:138-141 https://doi.org/10.1142/S1088424605000204
  23. Ohse T, S Nagaoka, Y Arakawa, H Kawakami and K Nakamura. 2001. Cell death by reactive oxygen species generated from water-soluble cationic metalloporphyrins as superoxide dismutase mimics. J. Inorg. Biochem. 85(2):201-208(8) https://doi.org/10.1016/S0162-0134(01)00187-8
  24. Shin MJ, HJ Kim, SJ Yang. 2002. Arsenic trioxide induces apoptosis in chronic myelogenous leukemia K562 cells: possible involvement of p38 MAP kinase. J. Biochem. Mol. Biol. 35:377-383 https://doi.org/10.5483/BMBRep.2002.35.4.377
  25. Stojiljkovic I, BD Evavold and V Kumar. 2001. Antimicrobial properties of porphyrins. Expert Opin Investig Drugs. 10:309-320 https://doi.org/10.1517/13543784.10.2.309
  26. Szurko A, G Kramer-Marek, M Widel, A Ratuszna, J Habdas and P Kus. 2003. Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro. Acta. Biochim. Pol. 50(4):1165-1174
  27. Tome JPC, MGPMS Neves, AC Tome, JAS Cavaleiro, M Soncin, M Magaraggi, S Ferro and G Jori. 2004. Synthesis and antibacterial activity of new poly-S-lysine-Porphyrin conjugates. J. Med. Chem. 47:6649-6652 https://doi.org/10.1021/jm040802v
  28. Vicente MGH. 2001. Porphyrin-based sensitizers in the detection and treatment of cancer: recent progress. Curr. Med. Chem. Anticancer Agents. 1:175-194 https://doi.org/10.2174/1568011013354769
  29. Vzorov AN, DW Dixon, JS Trommel, LG Marzilli and RW Compans. 2002. Inactivation of human immunodeficiency virus type 1 by porphyrins. Antimicrob Agents Ch. 46: 3917-3925 https://doi.org/10.1128/AAC.46.12.3917-3925.2002
  30. Wyllie AH, JFR Kerr, AR Currie. 1980. Cell death: the significance of apoptosis. Int. Rev. Cytol. 68:251-306 https://doi.org/10.1016/S0074-7696(08)62312-8