식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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토끼풀에서 분리한 Peanut stunt virus의 성질

Some Properties of an Isolate of Peanut stunt virus Isolated from White Clover (Trifolium repens L.)

  • 정구호 (강원대학교 생물자원공학부) ;
  • 전용운 (강원대학교 생물자원공학부) ;
  • 최장경 (강원대학교 생물자원공학부) ;
  • 홍진성 (서울여자대학교 환경생명과학부) ;
  • 류기현 (서울여자대학교 환경생명과학부) ;
  • 이상용 (강원대학교 산림자원보호학과)
  • Jung, Goo-Ho (Division of Bio-resources Technology, Kangwon National University) ;
  • Jeon, Yong-Woon (Division of Bio-resources Technology, Kangwon National University) ;
  • Choi, Jang-Kyung (Division of Bio-resources Technology, Kangwon National University) ;
  • Hong, Jin-Sung (Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Ryu, Ki-Hyun (Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Lee, Sang-Yong (Department of Forest Resources Protection, Kangwon National University)
  • 발행 : 2008.04.01
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초록

전형적인 모자이크 증상을 나타낸 토끼풀로부터 Peanut stunt virus(PSV)를 분리하여 Tr-PSV로 명명하였다. 이 연구에서는 Tr-PSV의 특성을 기주반응, 혈청학적 성질, dsRNA분석, RT-PCR 및 RFLP 분석 등을 통하여 지금까지 잘 알려진 ER-PSV Fny-CMV 및 LS-CMV와 비교하였다. Tr-PSV는 접종한 Nicotiana속 식물에서는 모두 전신감염되었으며, C. amaranticolor에서는 접종엽에 국부병반을 형성하였다. 그러나 동부에서는 대조로 이용한 ER-PSV와 마찬가지로 전신감염되어, 접종엽에 국부병반을 형성하는 대조의 CMV계통과는 구분되었다. Tr-PSV에 감염된 N. benthamiana 잎으로부터 추출한 dsRNA는 대조의 PSV나 CMV와 유사한 분자 크기의 4종의 dsRNA가 확인되었고, satellite RNA의 존재는 인정되지 않았다. Cucumovirus 특이적 프라이머를 이용하여 증폭시킨 PCR산물은 약 950 bp의 cDNA가 얻어졌고, 이를 이용하여 조사한 RFLP패턴은 ER-PSV와 같았다. 이와 같은 RFLP분석과 Er-PSV의 항혈청을 이용한 혈청학적 성질의 결과로부터 Tr-PSV는 ER-PSV와 같은 서브그룹 I에 속하는 PSV의 한 계통으로 판단되었다. 국내에서 토끼풀로부터 PSV가 분리 동정된 것은 이 논문이 처음이다.

An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.

키워드

참고문헌

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피인용 문헌

  1. Characterization of Cucumber mosaic virus Isolated from Trifolium repens in Korea vol.22, pp.1, 2016, https://doi.org/10.5423/RPD.2016.22.1.55