Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Melanogenesis Inhibition by Forsythiae Fructus Extract in Human Melanoma Cells

인체 멜라닌세포주에서 연교(連翹) 추출물의 멜라닌생성 억제기전 연구

  • Jo, Mi-Gyeong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 조미경 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2008.04.25
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Abstract

In this study, we have investigated the hypo-pigmentary mechanism of methanol extract of Forsythiae Fructus in human melanocyte cell line, HM3KO. Treatment of HM3KO cells with Forsythiae Fructus extract markedly inhibited melanin biosynthesis in a dose-dependent manner. Decreased melanin contents occurred through the decrease of tyrosinase protein and activity. The mRNA levels of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also reduced by Forsythiae Fructus extract. Moreover, the level of intracellular cyclic AMP (cAMP) was significantly decreased by treatment of Forsythiae Fructus extract. These results suggest that Forsythiae Fructus reduces melanin synthesis by down regulation of tyrosinase mRNA transcription, and this is closely related to the cAMP-dependent pathway.

Keywords

References

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