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Melanogenesis Inhibition by Forsythiae Fructus Extract in Human Melanoma Cells  

Jo, Mi-Gyeong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.2, 2008 , pp. 371-376 More about this Journal
Abstract
In this study, we have investigated the hypo-pigmentary mechanism of methanol extract of Forsythiae Fructus in human melanocyte cell line, HM3KO. Treatment of HM3KO cells with Forsythiae Fructus extract markedly inhibited melanin biosynthesis in a dose-dependent manner. Decreased melanin contents occurred through the decrease of tyrosinase protein and activity. The mRNA levels of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also reduced by Forsythiae Fructus extract. Moreover, the level of intracellular cyclic AMP (cAMP) was significantly decreased by treatment of Forsythiae Fructus extract. These results suggest that Forsythiae Fructus reduces melanin synthesis by down regulation of tyrosinase mRNA transcription, and this is closely related to the cAMP-dependent pathway.
Keywords
Forsythiae Fructus; cAMP; tyrosinase; TRP-1;
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1 김창민, 신민교, 안영균, 이경호외. 중약대사전, 10권, pp 4871-4872, 1997
2 Rim, Y.S., Park, Y.M., Park, M.S., Kim, K.Y., Kim, M.J., Choi, Y.H. Screening of antioxidant and antimicrobial activity in native plants. Korean J. Medicinal Crop. Sci. 8(4):342-350, 2000
3 Schinella, G.R., Tournier, H.A., Prieto, J.M., Mordujovich, D., Rios, J.L. Antioxidant activity of anti-inflammatory plant extracts. Life Sci. 70(9):1023-1033, 2002   DOI   ScienceOn
4 조미경, 안병상, 문연자, 우원홍. 연교 메탄올추출물의 멜라닌생성 억제효과. 대한한방피부미용학회지 1(1):41-52, 2005
5 Hearing, V.J. Regulation of melanin formation. In: Nordlund, J.J., Boissy, R.E., Hearing, V.J., King, R.A., Ortonne, J.P., editors. The pigmentary system: physiology and pathophysiology. 1st ed. New York, Oxford University Press, pp 423-438, 1998
6 Hearing, V.J. Biochemical control of melanogenesis and melanosomal organization. J. Invest. Dermatol. Symp. Proc. 4: 24-28, 1999
7 윤태진, 이무형. All-trans-retinoic Acid가 ${\alpha}$-MSH로 자극한 배양 정상 인체 멜라닌세포의 성장과 cAMP 농도에 미치는 영향. 대한피부과학학회지 37(8):1017-1028, 1999
8 Hosoi, J., Abe, E., Suda, T., Kuroki, T. Regulation of melanin synthesis of B16 mouse melanoma cells by $1{\alpha}$,25-dehydroxy-vitamin D3 and retinoic acid, Cancer Res. 45: 1474-1478, 1985
9 Funasaka, Y., Chakraborty, A.K., Komoto, M., Ohashi, A., Ichihashi, M. The depigmenting effect of ${\alpha}$-tocopherol ferulate on human melanoma cells. British. J. Dermatolohy 141: 20-29, 1999   DOI   ScienceOn
10 Masamoto, Y., Ando, H. et al. Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L. Biosci. Biotechnol. Biochem. 67: 631-634, 2003   DOI   ScienceOn
11 Medrano, E.E., Yang, F., et al. Terminal differentiation and senescence in the human melanocyte: Repression of tyrosine-phosphorylation of the extracellular signal-regulated kinase 2 selectively defines the two phenotypes. Mol. Biol. Cell 5: 497-509, 1994   DOI
12 Bertolotto, C., Busca, R., Abbe P, Bille K, Aberdam E, Ortonne JP, Ballotti R. Different cis acting elements are involved in the regulation of TRP-1 and TRP-2 promoter activities by cyclic AMP : pivotal role of M boxes (GTCATGTGCT) and of microphthalmia. Mol. Cell Biol. 18: 694-702, 1998   DOI
13 Martinez-Esparza, M., Jimenez-Cervantes, C., Solano, F., Lozano, J.A., Garcia-Borron, J.C. Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells. Eur. J. Biochem. 255(1):139-146, 1998   DOI   ScienceOn
14 Gabriela, N., Raymond, A.D. et al. Tyrosinase-related protein-2 and -1 are trafficked on distinct routes in B16 melanoma cells. Res. Biochem. Biophysical. 328: 914-921, 2005   DOI   ScienceOn
15 Lee, E.B., Keum, H.J. Pharmacological studies on Forsythae Fructus. Kor. J. Pharmacogn. 19(4):262-269, 1988
16 Hearing, V.J., Jimenez, M. Mammalian tyrosinase-the critical regulatory control point melanocyte pigmentation. Int. J. Biochem. 19: 1141-1147, 1987   DOI   ScienceOn
17 Motokawa, T., Kato, T., Katagiri, T., Suzuki, I.. Meassenger RNA levels of melanogenesis associated genes in lentigo senilis lesions. J. Dermatological Science 37(2):120-123, 2005   DOI   ScienceOn
18 Maher, M.H., Weidong, X., et al. Activation of a cAMP pathway and induction of melanogenesis correlate with association of p16INK4 and p27KIP1 to CDKs, loss of E2F-Binding activity, and premature senescence of human melanocytes. Exp. Cell Res. 253: 561-572, 1999   DOI   ScienceOn
19 Li, Z.X., Wang, X.H., Zhao, J.H., Yang, J.F., Wang, X. Investigation on antibacterial activity of Forsythia suspense Vahl in vitro with Mueller-Hinton agar. Zhongguo Zhong Yao Za Zhi 25(12):742-745, 2000
20 Jimbow, K., Fitzpatrick, T.B., Wick, M. Biochemistry and physiology of melanin pigmentation. In: Goldsmith L.A., ed., Physiology and Molecular Biology of the skin. Oxford Univ. Press, New York, pp 873-909, 1991
21 Wakamatsu, K., Ito, S. Advanced chemical methods in melanin determination. Pigment Cell Res. 15: 174-183, 2002   DOI   ScienceOn
22 Wu, H., Park, HY. Protein kinase C-${\beta}$-mediated complex formation between tyrosinase and TRP-1. Biochem. Biophy. Res. Com. 311: 948-953, 2003   DOI   ScienceOn
23 Cui, X., Lee, S.J. et al. Effects of pituitary adenylate cyclase activating polypeptide 27 on cyclic AMP efflux and atrial dynamics in perfused beating atria. Eur. J. Pharmacol. 402: 129-137, 2000   DOI   ScienceOn
24 Lim, D.K., Choi, U., Shin, D.H. Antioxidative Activity of Ethanol Extract from Korean Medicinal Plants. Korean J. Food Sci. Technol. 8(4):342-350, 2000
25 Englaro, W., Rezzonico, R., Durand-Clément M, Lallemand D, Ortonne JP, Ballotti R. Mitogen-activated protein kinase pathway and AP-1 are activated during cAMP-induced melanogenesis in B-16 melanoma cells. J. Biol. Chem. 270: 24315-24320, 1995   DOI   ScienceOn
26 Kazuhisa, M., Yoshihiro, Y., et al. Comparison of the melanogenesis in human black and light brown melanocytes. J. Sci. Dermatol. 14: 199-206, 1997   DOI   ScienceOn
27 Fitzpatrick, T.B., Szabo, G., Seiji, M., Quevedo, W.C. Biology of the melanin pigmentary system. In: Fitzpatrick, T.B., ed., Dermatology in General Medicine. Academic Press, New York, pp 131-163, 1979
28 Roser, B., Robert, B. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment cell Res. 13: 60-69, 2000   DOI   ScienceOn
29 Maeda, K., Tomita, Y., et al. Effects of staurosporine, PMA and A23187 on human melanocyte cultures with dibutyryl cyclic AMP. Br. J. Dermatol. 126: 118-124, 1992   DOI   ScienceOn
30 del Marmol, V., Ito, S., Jackson, I., Vachtenheim, J., Berr, P., Ghanem, G., Morandini, R., Wakamatsu, K., Huez, G. TRP-1 expression correlates with eumelanogenesis in human pigment cells in culture. Eur. Biochem. 327: 307-310, 1993
31 辛民敎. 臨床本草學. 永林社, pp 431, 1997
32 Kametama K, Takemura, T., Hamada, Y., Sakai, C., Kondoh, S., Nishiyama, S., Urabe, K., Hearing, J. Pigment production in murine melanoma cell is regulated by tyrosinase, tyrosinase- related protein 1(TRP-1), DOPAchrome tautomerase(TRP-2), and a melanogenic inhibitor. J. Invest. Dermatol. 2: 126-131, 1993
33 Commo, S., Gaillard, O., Thibaut, S. Absence of TRP-2 in Melanogenic Melanocytes of Human Hair. Pigment Cell Res. 17: 488-497, 2004   DOI   ScienceOn
34 Ozaki, Y., Rui, J., Tang, Y.T. Antiinflammatory effect of Forsythia suspensa V (AHL) and its active principle. Biol. Pharm. Bull. 23(3):365-367, 2000   DOI   ScienceOn
35 Hemesath, T.J., Price, E.R., Takemoto C, Badalian T, Fisher DE. MAP kinase links the transcription factor microphthalmia to c-kit signalling in melanocytes. Nature 391: 298-301, 1998   DOI   ScienceOn