Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Protective Effect of Neuronal Cell on Glutamate-induced Oxidative Stress from Viola mandshurica Extracts

Glutamate에 의한 산화적 스트레스로부터 신경세포를 보호하는 제비꽃 추출물의 영향

  • Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Han, Chang-Suk (Laboratory of Herbal Medicine, Daehandang Herb Clinic) ;
  • Han, Dong-Youl (Laboratory of Herbal Medicine, Daehandang Herb Clinic) ;
  • Park, Eun-Ju (Department of Food and Nutrition Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 이미라 (경남대학교 식품생명학과) ;
  • 한창석 (대한당한약방 한약연구실) ;
  • 한동열 (대한당한약방 한약연구실) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Published : 2008.03.31
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Abstract

The present study describes glutamate which is known as excitatory neurotransmitter is related with oxidative damages and the Viola mandshurica extracts. Showed protective effects against glutamate-induced cytotoxicity. The protective effect of antioxidant on the glutamate treated N18-RE-I05 cells was determined by a MTT reduction assay. The neuroprotective effect of methanol, ethanol, and acetone extracts from V. mandshurica against glutamate-induced cytotoxicity was assessed by the results of an MTT reduction assay. Among the three extracts, the acetone extract showed the highest protective effect by the results of an lactate dehydrogenase release assay. Therefore, these results suggest that V. mandshurica extracts could be a new potential candidate against glutamate-induced oxidative stress.

본 연구에서는 신경독소일 뿐만 아니라 흥분성 신경전달물질로 잘 알려져 있는 glutamate 세포독성이 산화적 손상과 관련하고 있고, 여기에 방어효과를 보이는 제비꽃 추출물에 관하여 연구하였다. MTT reduction assay를 통하여 glutamate의 세포독성을 확인하였고 ascorbic acid와 같은 대표적인 항산화제를 처리한 후 광학 현미경을 이용한 형태학적 변화를 관찰하였다. N18-RE-105 세포주에 최종 농도 20mM의 glutamate를 처리 하면 40.8% 의 생존율을 보이는데 반하여 ascorbic acid 500 ${\mu}M$ 최종농도로 처리하였을 때 85.3%의 세포 생존율을 확인할 수 있었다. 그리고 신경세포 보호효과를 가지는 제비꽃을 methanol, ethanol, acetone 추출한 뒤 MTT reduction assay를 이용하여 활성을 확인하였으며 그 중 acetone 추출물을 최종농도 50, 100 ${\mu}g/ml$를 처리 시 76.8%, 79.4%로 가장 높은 세포 생존율을 확인할 수 있었다. 이 결과는 N18-RE-105 세포주의 형태학적 변화와 LDH release assey에서도 일치하는 결과를 확인하였다.

Keywords

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