Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Growth Inhibitory Activity of Enterococcus faecium Isolated from Bovine Intestinal Tract against Enterobacter sakazakii

소 장관 유래 Enterococcus faecium의 Enterobacter sakazakii에 대한 생육저해활성

  • Park, Ju-Hui (Department of Food Science and Biotechnology, Kyungwon University, Gene Bank of Animal Pathogens, Kyungwon University) ;
  • Yoon, Sung-Sik (Department of Biological Resources and Technology, Yonsei University, Institute of Functional Biomaterials and Biotechnology, Yonsei University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University, Gene Bank of Animal Pathogens, Kyungwon University)
  • 박주희 (경원대학교 식품생물공학과, 경원대학교 동물병원성미생물유전자은행) ;
  • 윤성식 (연세대학교 생물자원공학과, 연세대학교 생리활성소재연구소) ;
  • 박영서 (경원대학교 식품생물공학과, 경원대학교 동물병원성미생물유전자은행)
  • Published : 2008.03.30
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Abstract

A lactic acid bacterium showing growth inhibitory activity against Enterobacter sakazakii was isolated from bovine intestinal tracts. From biochemical and molecular biological studies, the isolate was identified and named as Enterococcus faecium JH95. This strain was resistant to kanamycin and streptomycin at a concentration of $100{\mu}g/mL$. E. faecium JH95 had high antimicrobial activity against food-borne pathogens such as Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, and Clostridium perfrigens. The culture supernatant of this strain did not have antimicrobial activity. The culture broth of this strain failed to show the antimicrobial activity by heat treatment at $100^{\circ}C$ for 5 min or by pretense treatments for 2 hr. This result suggested that the putative antimicrobial substance produced by E. faecium JH95 is likely a protein which is not secreted into culture medium.

소 장관으로부터 분리한 유산균 중에서 Enterobacter sakazakii에 생육저해활성을 나타내는 균주를 분리한 후 동정한 결과 Enterococcus faecium으로 동정되었고 E. faecium JH95로 명명하였다. 본 균주는 kanamycin과 streptomycin에 대해 $100{\mu}g/mL$가지 매우 높은 내성을 나타내었다. 본 균주의 배양액은 L. monocytogenes, C. perfringens와 E. sakazakii에 대하여 높은 항균 활성을 나타내었으며, S. typhimurium, S. aureus와 E. coli O157:H7에 대해서도 항균활성을 나타내었다. 본 균주의 배양 상등액은 항균활성을 보이지 않았으며, 배양액이 지니고 있는 항균활성은 $100^{\circ}C$에서 5분간 가열하거나 단백질 가수분해효소 처리에 의해 소실되어 식중독 미생물에 대한 생육저해물질이 단백질인 것으로 추정되었다.

Keywords

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