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http://dx.doi.org/10.5851/kosfa.2008.28.1.99

Growth Inhibitory Activity of Enterococcus faecium Isolated from Bovine Intestinal Tract against Enterobacter sakazakii  

Park, Ju-Hui (Department of Food Science and Biotechnology, Kyungwon University, Gene Bank of Animal Pathogens, Kyungwon University)
Yoon, Sung-Sik (Department of Biological Resources and Technology, Yonsei University, Institute of Functional Biomaterials and Biotechnology, Yonsei University)
Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University, Gene Bank of Animal Pathogens, Kyungwon University)
Publication Information
Food Science of Animal Resources / v.28, no.1, 2008 , pp. 99-104 More about this Journal
Abstract
A lactic acid bacterium showing growth inhibitory activity against Enterobacter sakazakii was isolated from bovine intestinal tracts. From biochemical and molecular biological studies, the isolate was identified and named as Enterococcus faecium JH95. This strain was resistant to kanamycin and streptomycin at a concentration of $100{\mu}g/mL$. E. faecium JH95 had high antimicrobial activity against food-borne pathogens such as Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, and Clostridium perfrigens. The culture supernatant of this strain did not have antimicrobial activity. The culture broth of this strain failed to show the antimicrobial activity by heat treatment at $100^{\circ}C$ for 5 min or by pretense treatments for 2 hr. This result suggested that the putative antimicrobial substance produced by E. faecium JH95 is likely a protein which is not secreted into culture medium.
Keywords
Enterococcus faecium; Enterobacter sakazakii; antimicrobial activiey; minimal inhibitory concentration;
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