Honeybee Acute and Residual Toxicity of Pesticides Registered for Strawberry

딸기용 농약의 꿀벌에 대한 급성독성 및 엽상잔류독성

  • Kim, Byung-Seok (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Park, Yoen-Ki (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Lee, Yong-Hoon (Department of Agricultural Biology, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Joeng, Mi-Hye (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • You, Are-Sun (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Yang, Yu-Jung (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Kim, Jin-Bae (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Kwon, Oh-Kyung (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Ahn, Young-Joon (School of Agricultural Biotechnology, Seoul National University)
  • 김병석 (농업과학기술원 농산물안전성부) ;
  • 박연기 (농업과학기술원 농산물안전성부) ;
  • 이용훈 (농업과학기술원 농업생물부) ;
  • 정미혜 (농업과학기술원 농산물안전성부) ;
  • 유아선 (농업과학기술원 농산물안전성부) ;
  • 양유정 (농업과학기술원 농산물안전성부) ;
  • 김진배 (농업과학기술원 농산물안전성부) ;
  • 권오경 (농업과학기술원 농산물안전성부) ;
  • 안용준 (서울대학교 농생명공학부)
  • Published : 2008.09.30

Abstract

Lack of honey bee toxicity data for most pesticide products used for strawberry restricts to predict the adverse effects to foraging honey bee after treatment of pesticide in plastic house. This study was conducted to evaluate the actual risk of worker honey bees (Apis mellifera L.) through acute contact toxicity test, acute oral toxicity test and toxicity of residues on foliage test with 21 pesticide products. The mortality of honeybee sprayed with 6 pesticides including dichlofluanid WP showed significantly different from control at recommended application rate in acute contact toxicity test at 24 hours after treatment. Fenpropathrin EC and milbemectin EC treatment groups showed more than 25% mortalities at recommended application rate in acute oral toxicity test. In toxicity of residues on foliage test, only fenpropathrin EC treatment group showed more than 25% mortalities at 10 days after treatment at recommended application rate. It was concluded that the most toxic route to exposure for honey bee is direct contact exposure to sprayed pesticides. Safety interval for honey bee was established by concerning the results of these tests.

시설재배 딸기에 사용하는 농약이 화분매개를 위해 투입된 꿀벌(Apis mellifera L.)에 미치는 영향을 조사하기 위해 꿀벌에 노출될 가능성이 있는 21종의 농약을 선정하여 꿀벌 급성독성시험과 엽상잔류독성시험을 실시하였다. 표준살포농도로 분무처리한 급성접촉독성시험에서는 24시간 후에 꿀벌치사율이 대조군과 유한 차이를 보인 농약은 dichlofluanid WP 등 6종이었고, 48시간 후에는 fenpropathrin EC 등 7종이었다. 급성 섭식독성시험에서는 fenpropathrin EC만이 꿀벌에 독성을 보여주었으며 milbemectin EC는 배량처리군에서 대조군과 비교하여 유의한 치사율 차이를 보여주었다. 또한 엽상잔류독성시험에서는 fenpropathrin EC만이 10일 이상 장기간 잔류독성을 보였다. 이상의 결과를 종합하여 21종 제품농약에 대한 살포 후 꿀벌안전방사기간을 제안하였다.

Keywords

References

  1. Atkins, E. L. (1992) Injury to honey bee by poisoning. In: Graham J. E., The hive and the Honey bee. Dadant and Sons, Hamilton, pp. 1153-1208
  2. Devillers, J. and M. Pham-Dellegue (2002) Honey bee: estimating the environmental impact of chemicals. Tayler & Francis, London, p. 332
  3. Gough, H. J., E. C. McIndoe and G. B. Lewis (1994) The use of dimethoate as a reference compound in laboratory acute toxicity tests on honey bee (Apis mellifera L.) 1981-1992. Journal of Apicultural Research 33(2):119-125 https://doi.org/10.1080/00218839.1994.11100859
  4. Johansen, C. A. and D. F. Mayer (1990) Pollinator protection: a bee & pesticide handbook. Wicwas Press, Connecticut, p. 212
  5. OECD (1998) OECD guidelines for the testing of chemicals No.213 Honey bee acute oral toxicity test. Paris, France
  6. OECD (1998) OECD guidelines for the testing of chemicals No.214 Honey bee acute contact toxicity test. Paris, France
  7. Iwasa, T., N. Motoyama, J. T. Ambrose and R. M. Roe (2004) Mechanism for the diferential toxicity of neonicotinoid insecticides in the honey bee, Apis mellifera. Crop protection 23:371-378 https://doi.org/10.1016/j.cropro.2003.08.018
  8. Tomlin, C. (2000) The pesticide manual (12th ed.), The British Crop Protection Council, U.K
  9. USEPA (1996a) Ecological effect test guidelines OPPTS 850.3020 Honey bee acute contact toxicity. Washington DC, USA
  10. USEPA (1996b) Ecological effect test guidelines OPPTS 850.3030 Honey bee toxicity of residue on foliage. Washington DC, USA
  11. USEPA (2000) Notice to manufacturers, producers, formulators and registrants of pesticide products-Bee precautionary labeling statements. Pesticide registration Notice 2000-XX (draft). Washington DC, USA
  12. 농업과학기술원 (1998) 1998년도 시험연구사업보고서
  13. 농촌진흥청 (2007) 농약관리법령 고시 훈령집 p. 739
  14. 심용구 (1998) 꿀벌(Apis mellifera L.)의 수분활동 유발요인 및 결실에 미치는 영향. 경북대학교 농학박사학위논문
  15. 한국작물보호협회 (2007) 농약사용지침서 p. 823