The Acute and Chronic Toxicity Studies of Herbicide, Molinate to Waterfleas

Molinate의 물벼룩에 대한 급성 및 만성독성 연구

  • Shin, On-Sup (Department of Crop Life Safety, National Institute of Agricultural Science and Technology) ;
  • Kim, Byung-Seok (Department of Crop Life Safety, National Institute of Agricultural Science and Technology) ;
  • Park, Yeon-Ki (Department of Crop Life Safety, National Institute of Agricultural Science and Technology) ;
  • Park, Kyung-Hoon (Department of Crop Life Safety, National Institute of Agricultural Science and Technology) ;
  • Lee, Je-Bong (Department of Crop Life Safety, National Institute of Agricultural Science and Technology) ;
  • Kyung, Kee-Sung (College of Agriculture, Life and Environmental Sciences, Chungbuk National University) ;
  • Ahn, Young-Joon (School of Agricultural Biotechnology, Seoul National University)
  • 신진섭 (농업과학기술원 농산물안전성부) ;
  • 김병석 (농업과학기술원 농산물안전성부) ;
  • 박연기 (농업과학기술원 농산물안전성부) ;
  • 박경훈 (농업과학기술원 농산물안전성부) ;
  • 이제봉 (농업과학기술원 농산물안전성부) ;
  • 경기성 (충북대학교 농업생명환경대학) ;
  • 안용준 (서울대학교 농업생명과학대학)
  • Published : 2008.09.30

Abstract

To assess the impact of molinate on freshwater aquatic organisms, acute and chronic toxicity studies for waterfleas were conducted. In acute toxicity studies for Daphnia magna, and Moina macrocopa, the 48-h $EC_{50}$ values were 11.4 and 8.3 mg/L respectively. And in reproduction toxicity studies for the same species, the NOEC's were 2.5 and 2.0 mg/L respectively. These results suggest that waterfleas have simillar sensitivity to molinate. On the other hand, the NOEC for 3-generation toxicity of moina macrocopa 0.16 mg/L, was much lower than those of acute values. This studies concludes that molinate has minimal risk to waterfleas in river.

우리나라 하천수 중 검출빈도가 높게 검출되고 있는 제초제 molinate의 담수 무척추동물에 대한 영향을 평가하고자 물벼룩에 대한 급 만성독성시험 및 다세대노출시험을 수행하였다. Molinate는 담수 무척추동물인 물벼룩 Daphnia magna 와 Moina macrocopa,에 대한 $EC_{50}$(48시간)이 각각 11.4와 8.3 mg/L, 번식독성 무영향농도(NOEC)가 2.5와 2.0 mg/L로서 종간 감수성에 있어 큰 차이를 보이지 않았으며 M. macrocopa에 대한 3세대노출시험의 NOEC은 0.16 mg/L이었다. 하천에서 molinate에 의한 물벼룩에 대한 위해 가능성은 낮은 것으로 판단된다.

Keywords

References

  1. Dodson, S. I., C. M. Merritt, J. P. Shannahan and C. M. Shults (1999) Low exposure concentrations of atrazine increase male production in Daphnia pulicaria. Environ. Toxicol. Chem. 18(7):1568-1573 https://doi.org/10.1897/1551-5028(1999)018<1568:LECOAI>2.3.CO;2
  2. EDSTAC (Endocrine Disruptor Screening and Testing program Advisory Committee) (1998) EDSTAC Final report. USEPA, Washington DC
  3. Finney, D. J. (1971) Estimation of the median effective dose, pp. 19-47, In Probit Anaysis. Cambridge University Press, Cambridge, England
  4. LeBlank, G. A. and J. B. McLachlan (1999) Molt-independent growth inhibition of Daphnia magna by a vertebrate antiandrogen. Environ. Toxicol. Chem. 18(7):1450-1455 https://doi.org/10.1897/1551-5028(1999)018<1450:MIGIOD>2.3.CO;2
  5. Miyamoto, J. (ed.) (1998) 化學物質과內分泌攪亂. 化學工業日報社. 3-7
  6. OECD (1984) Daphnia sp., acute immobilization test and reproduction test. OECD guideline for testing of chemicals No.202
  7. OECD (1996) Daphnia magna reproduction test, OECD guideline for testing of chemicals Proposal for updated guideline No.211. TG\DAPHNIA\9678.DOC
  8. Ross, L. J. and R. J. Sava (1986) Fate of thiobencarb and molinate in rice fields. J. of Environmental auality. 15:220-224
  9. USEPA (1992a) Aquatic invertbrate acute toxicity test, freshwater Daphnid, Ecological Effects Test Guidelines. OPPTS 850.1010
  10. USEPA (1992b) Daphnid chronic toxicity test, Ecological Effects Test Guidelines. OPPTS 850.1300
  11. USEPA (2001) EFED's RED chapter for molinate (Reregistration Case #818845)
  12. Zou, E. and M. Fingerman (1997) Synthetic estrogenic agents do not interfere with sex differentiation but do inhibit molting of the cladoceran, Daphnia magna. Bull. Environ. Contam. Toxicol. 58:596-602 https://doi.org/10.1007/s001289900376
  13. 한국작물보호협회 (2007) 농약사용지침서
  14. 한국작물보호협회 (2006) 농약연보
  15. 한국작물보호협회 (2001) 시험연구사업보고서: 제초제 Molinate의 잔류량 조사