The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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High Concentration of Sodium Chloride Increases on Survival of Non-pathogenic Erwinia carotovora subsp. carotovora 9-3 during Drying and Storage

비병원성 Erwinia carotovora subsp. carotovora 9-3의 건조 및 저장의 과정에서 생존에 미치는 염 효과

  • Park, Kyoung-Soo (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Gun-Ju (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Shin, Yun-Ju (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Sik (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Cha, Jae-Soon (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • 박경수 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김건주 (충북대학교 농업생명환경대학 식물의학과) ;
  • 신윤주 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김식 (충북대학교 농업생명환경대학 식물의학과) ;
  • 차재순 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2008.12.31
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Abstract

Dry formulation is a limiting step for successful development of microbial bio-pesticides with the antagonistic Gram-negative bacteria because their survival rates are too low during drying and storage. The high concentration of sodium chloride (NaCl) in culture medium that induces osmolyte in bacterial cells is known to increase of survival rate during drying in many Gram-negative bacteria. Effect of NaCl on survival of antagonistic non-pathogenic Erwinia carotovora subsp. carotovora 9-3 (Ecc 9-3) during drying and storage was studied. Growth rate of Ecc 9-3 was not much different up to 0.5 M NaCl in NB while it was lower significantly with 0.7 M NaCl. Survival rates were twice and 3 times higher with 0.5 M NaCl than with no additional NaCl during drying at room temperature and freeze-drying, respectively. Survival rate was also higher with high NaCl in culture medium during storage after drying. It was not much different on storage at $4^{\circ}C$ both of drying at room temperature and freeze-drying. However, the survival rate was higher on storage at $27^{\circ}C$ and $37^{\circ}C$ with high NaCl concentration. Among the additives tested, lactose affects most to survival rate both of drying at room temperature and freeze-drying, and dextrin influenced significantly to survival rate of drying at room temperature.

길항력이 있는 그람음성균들의 생물적 방제제로 성공적인 개발은 균의 건조와 저장시의 낮은 생존을 때문에 건조제형으로 만드는 것은 제한되어있다. 그람음성균의 배양시 높은 염(NaCl)의 첨가는 삼투압 조절제인 osmolyte의 생성을 유도하여 건조 시 생존율을 높인다고 알려져 있다. 그람음성의 길항력을 내는 비병원성 Erwinia carotovora subsp. carotovora(Ecc) 9-3의 건조와 저장시의 염의 영향에 관해 연구하였다. Ecc 9-3의 생장률은 0.5 M의 염이 첨가되어 있는 배지에서 큰 차이가 없었지만 0.7 M의 염이 첨가된 배지에서는 급격하게 낮아진 것을 확인하였다. 건조와 저장시의 생존율은 0.5 M의 염을 넣은 배지가 염을 넣지 않은 배지보다 상온건조와 동결건조에서 각각 2배 그리고 3배의 높은 생존율을 얻었다. 건조 후 저장에서도 역시 염이 높을수록 Ecc 9-3의 생존율이 더 높았다. 그리고 상온건조와 동결건조 모두 $4^{\circ}C$에 저장하였을 때 생존율에 큰 차이가 없었다. 그러나 생존율은 $27^{\circ}C$, $37^{\circ}C$에 저장하였을 때 염의 농도가 높을수록 생존율이 증가하였다. 첨가제 실험에서는 lactose가 동결건조와 상온건조 모두에 영향을 주었고 dextrin은 상온건조에 큰 영향을 주었다.

Keywords

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