Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of the Combination Feeding of β-glucan and Multi-species Probiotics on Growth Performance and Microflora in Broiler Chickens

효모변이균주 유래 β-글루칸과 복합균종 생균제의 혼합급여가 육계의 생산성 및 장내 균총에 미치는 영향

  • Cho, B.I. (Animal Resources Research Center, College of Animal Bioscience & Technology, Konkuk University) ;
  • You, S.J. (Animal Resources Research Center, College of Animal Bioscience & Technology, Konkuk University) ;
  • Kim, E.J. (Animal Resources Research Center, College of Animal Bioscience & Technology, Konkuk University) ;
  • Ahn, B.K. (Animal Resources Research Center, College of Animal Bioscience & Technology, Konkuk University) ;
  • Paik, H.D. (Animal Resources Research Center, College of Animal Bioscience & Technology, Konkuk University) ;
  • Yun, C.W. (College of Life Science and Biotechnology, Korea University) ;
  • Chang, H.I. (College of Life Science and Biotechnology, Korea University) ;
  • Kim, S.W. (College of Life Science and Biotechnology, Korea University) ;
  • Kang, C.W. (Animal Resources Research Center, College of Animal Bioscience & Technology, Konkuk University)
  • 조병임 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 유선종 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 김은집 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 안병기 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 백현동 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 윤철원 (고려대학교 생명과학대학) ;
  • 장효일 (고려대학교 생명과학대학) ;
  • 김승욱 (고려대학교 생명과학대학) ;
  • 강창원 (건국대학교 동물생명과학대학 동물자원연구센터)
  • Published : 2008.02.01
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Abstract

This experiment was carried out to investigate the combination feeding of β-glucan and multi-species probiotics on growth performance, various lipid concentrations of serum, antibody production and cecal microbial profiles in broiler chicks. A total of six hundred 1-d-old male broiler chicks were divided into five groups, placed into four pens per group(30 birds per pen) and fed one of five non-medicated experimental diets(T1, 0.15% multi-species probiotics; T2, 0.1% β-glucan+0.15% multi-species probiotics; T3, 0.3% multi-species probiotics; T4, 0.1% β-glucan+0.3% probiotics or devoid them as control) for 5 wk. There was no significant difference in feed intake among the groups. The average weight gains and FCR in groups fed diet containing 0.3% probiotics were significantly improved(p<0.05) than control in finisher period(22-35d). The concentration of serum cholesterol ester in groups fed 0.3% probiotics were significantly lowered(p<0.05) as compared to that of the control. Relative weights of liver, spleen, bursa of Fabricius, breast and leg were not influenced by the dietary treatments. The average ND or IB antibody titers in groups fed diets containing β-glucan and probiotics were tended to be increased, but not significantly. The number of cecal lactic acid bacteria was significantly increased(p<0.05) by the dietary β- glucan and probiotics. These results indicated that dietary β-glucan and probiotics exerted a growth- promoting and immune-enhancing effects on broiler chicks. In addition, yeast derived β-glucan, and multi-species probiotics modulated the profiles of cecal microflora, reflecting potential alternative substances to replace antibiotics for feeding broiler.

본 실험에서는 복합균종 생균제를 단일급여하거나 효모변이균주에서 추출한 β-글루칸과 혼합급여했을 때 성장 성적, 혈액 내 지질 분획 농도, 항체생산 반응 및 장내 균총의 변화에 미치는 영향을 조사하였다. 모두 5개 처리구에 4반복 반복당 30수 씩, 총 600수의 1일령 Ross 수평아리를 공시하여 35일간 사양실험을 실시하였다. 실험사료는 옥수수와 대두박을 위주로한 항생제 무첨가 시판사료를 급여하는 대조구와 T1 처리구에는 복합균종 생균제만을 0.15%, T2 처리구에는 β-글루칸 0.10%+복합균종 생균제 0.15%, T3 처리구에는 복합균종 생균제만을 0.30%, T4 처리구에는 β-글루칸 0.10%+복합균종 생균제 0.30%를 혼합급여하였다. 실험 기간 중의 증체량, 사료 섭취량 및 사료요구율을 평가하였고, 실험 4주째에 ND 및 IB를 분무백신한 후 항체 생성량을 조사하였다. 맹장 내용물을 채취하여 장내 균총 변화에 미치는 영향을 조사하였다.사료 섭취량에서는 처리간에 큰 차이가 없었으나 실험후기에서는 증체량 및 사료요구율은 대조구에 비해 모든 처리구에서 유의하게 개선되거나(P<0.05) 개선되는 경향을 나타내었다. 간, 비장, F낭, 가슴근육 및 다리부위의 상대적 중량에서는 처리간에 큰 차이가 없는 것으로 나타났다. 혈중 콜레스테롤 에스터 농도는 대조구에 비해 모든 복합균종 생균제 0.3% 첨가구(T3 및 T4)에서 유의하게 감소하였고(P<0.05), 유리형 콜레스테롤 농도 역시 감소하는 경향을 나타내었다. ND 및 IB 항체 생산량은 대조구에 비해 유의한 차이 없이 증가하는 경향을 나타내었다. 총 균 및 coliform bacteria 수에서는 처리간에 큰 차이가 없었으나, lactic acid bacteria 수는 대조구에 비해 모든 처리구에서 유의하게 증가하는 결과가 관찰되었다(P<0.05). 본 실험에서는 육계사료 내 복합균종 생균제의 단일급여 또는 β-글루칸과의 혼합급여가 증체 및 사료요구율을 개선시키는 효과를 나타내었다. 특히 복합균종 생균제 급여에 의해 혈중 콜레스테롤 농도가 유의하게 감소하고, 맹장 내 lactic acid bacteria 수가 유의하게 증가하는 효과를 나타났다.

Keywords

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