Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Selenium Feeding on Selenium Concentration of Blood and Velvet Antler in Sika deer(Cervus nippon)

셀레늄 급여가 꽃사슴의 혈액과 녹용내 셀레늄 함량에 미치는 영향

  • Kim, Bu-Yeon (College of Animal Bioscience & technology, Konkuk University) ;
  • Kim, Myeong-Hwa (Korea Nokyung Research Center, Konkuk University) ;
  • Jeon, Byong-Tae (Korea Nokyung Research Center, Konkuk University) ;
  • Moon, Sang-Ho (Korea Nokyung Research Center, Konkuk University) ;
  • Lee, Hong-Gu (Natural Resource and Life sciences, Busan National University) ;
  • Lee, Sang-Rak (College of Animal Bioscience & technology, Konkuk University)
  • 김부연 (건국대학교 동물생명과학대학) ;
  • 김명화 (건국대학교 한국녹용연구센터) ;
  • 전병태 (건국대학교 한국녹용연구센터) ;
  • 문상호 (건국대학교 한국녹용연구센터) ;
  • 이홍구 (부산대학교 생명자원과학대학) ;
  • 이상락 (건국대학교 동물생명과학대학)
  • Published : 2008.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the effects of selenium feeding and supplementation in diet on the concentration of selenium in blood and velvet antler of spotted deer(Sika deer). Three spotted deer were fed high selenium concentration(6mg/kg DM). Absorption and retention rates of selenium were examined by evaluating selenium concentrations in feces and urine. Stress-related hormones and serum biochemical parameters in blood were also evaluated for the purpose of detecting any negative effect by the high level of selenium feeding. Eight spotted deers were randomly assigned to two groups and were fed with one of two diets for 20 days, which were with or without the addition of 6mg selenium /kg diet. Concentration of selenium in velvet antler was evaluated. Selenium concentration in blood of spotted deer fed high level selenium for 30 days was significantly increased(p<0.05), retention rate of selenium reached 59.15%. No differences in level of stress-related hormone and biochemical parameters(NEFA, ALT, AST) in blood were observed by feeding high level selenium. The diet with selenium significantly increased concentrations of selenium in top(0.11 vs 0.45ppm; p<0.001), middle(0.08 vs 0.21ppm; p<0.01) and basepart(0.08 vs 0.15ppm; p<0.05) of velvet antler.

본 연구는 꽃사슴에게 고농도의 셀레늄을 급여하였을 때 꽃사슴의 혈액, 녹용 및 생리반응에 미치는 영향을 평가하고자 실시하였다. 3두의 꽃사슴에게 고농도 셀레늄을 급여하였을 때의 혈액 내의 셀레늄 농도와 셀레늄 흡수율 및 축적률을 조사하고, 혈액 내 스트레스 관련호르몬 및 biochemical parameters를 조사하였다. 8두의 수컷 꽃사슴을 공시하여 셀레늄을 첨가하지 않은 사료를 급여한 구와 셀레늄을 사료 kg당 6mg을 첨가한 사료를 20일간 급여한 구로 나누어 생산한 녹용의 셀레늄 함량을 조사하였다. 꽃사슴에게 고농도 셀레늄 급여는 급여 30일 이후에 혈액 중의 셀레늄 농도를 유의하게 증가시켰고(p<0.05), 축적률은 59.15%를 나타내었다.전 실험 기간 동안 고농도 셀레늄의 급여로 인한 스트레스 관련 호르몬 및 biochemical parameters의 변화는 나타나지 않았다. 녹용 내 셀레늄 함량은 대조구의 경우 상대 0.11ppm, 중대 0.08ppm, 하대에 0.08ppm을 셀레늄 급여구에서는 상대 0.45ppm(p<0.001), 중대 0.21ppm(p<0.01), 하대에 0.15ppm(p<0.05)으로 셀레늄 급여로 인하여 유의하게 증가하였으며, 상대에서의 셀레늄 함량이 가장 높았다(p< 0.05).이상의 결과로부터 꽃사슴에게 고농도의 셀레늄을 급여할 경우 녹용내로 다량의 셀레늄을 전이시킬 수 있는 것으로 판단되었고, 40일까지 고농도의 셀레늄 6mg/kg 농도 급여에 있어 생리적 중독 증상은 없었으나 혈중 소실정도를 감안해 볼 때 20일간 급여하는 것이 적합하다고 사료된다. 향후 셀레늄 강화 녹용 생산을 위해 급여하는 셀레늄의 최적 농도와 급여 기간에 대한 연구가 더 필요할 것으로 사료된다.

Keywords

References

  1. AOAC. 2000. Official Methods of Analysis(17th Ed.). Association of Official Analytical Chemists, Washington, DC
  2. Ashmore, C. R., Parker, W. and Doerr, L. 1972. Respiration of mitochondria isolated from dark-cutting beef: Postmortem changes. J. Anim. Sci. 34:46-48 https://doi.org/10.2527/jas1972.34146x
  3. Balm, P. H. M. 1990. Stress physiology in animals. Sheffield Academic Press Ltd., Sheffield, U. K.
  4. Carragher, J. F., Ingram, J. R., Matthews, L. R. and Schaare, P. R. 1997 Plasma cortisol responses to remote adrenocorticotropic hormone(ACTH) infusion in free-ranging red deer(Cervus elaphus). Domest Anim. Endocrinol. 1997 Jan; 14(1):63-71 https://doi.org/10.1016/S0739-7240(96)00095-1
  5. Chen, X., Yang, G., Wen, Z., Chen, J. and Ge, K. 1981. Relation of the selenium deficiency to the occurrence of Keshan disease. Spallholz, J. E., Martin, J. L., Ganther, H. E.(Ed.) Selenium in Biological and Medicine. Conn AVI Publish Co. Westport. pp. 171-175
  6. Clark, L. C., Jr. Combs, G. F., Turnbull, B. W., Slate, E. H., Chalker, D. K., Chow, J., Davis, L. S., Glover, R. A., Graham, G. F., Gross, E. G., Krongrad, A., Jr. Lesher, J. L., Park, H. K., Jr. Sanders, B. B., Smith, C. L. and Taylor, J. R. 1996. Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. A randomized controlled trial. Nutritional Prevention of Cancer Study Group. J. Am. Med. Assoc. Dec. 25 276(24):1957-63 https://doi.org/10.1001/jama.1996.03540240035027
  7. Combs, G. F. 2001. Selenium in global food systems. Br. J. Nutr. 85:517-547 https://doi.org/10.1079/BJN2000280
  8. Deer Industry New Zealand Annual Report 2006
  9. Diskin, C. J., Tomasso, C. L., Alper, J. C., Glaser, M. L. and Fliegel, S. E. 1979. Long-term selenium exposure. Arch. Intern. Med. 1979 39(7): 824-6
  10. Edmondson, A. J., Norman, B. B. and Suther, D. 1993. Survey of state veterinarians and state veterinary diagnostic laboratories for Se deficiency and toxicosis in animals. J. Am. Vet. Med. Assoc. 202:865-874
  11. Eklow, L., Jones, D. P., Thor, H. and Orrenius, S. 1981. Metabolism of hydrogen peroxide in isolated hepatocytes: relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated H2O2. Arch Biochem. Biophys. Sep. 210(2):505-16 https://doi.org/10.1016/0003-9861(81)90215-0
  12. Fayez, S., Sayegh, D. M. D., Gordon, M. S., Solomon, C. and Robert, W. D. 1974. Ultra-structure of intracellular mineralization in the deer's antler. Clinical Orthopaedics and Related Research. Number 99 March-April
  13. Georgievskii, A. S. 1982. Contribution of Russian military physicians to the establishment and development of the preventive trend in medicine. Sov Zdravookhr. 10:66-70
  14. Grace, N. D., Ankenbauer-Perkins, K. L., Alexander, A. M. and Marchant, R. M. 2001. Relationship between blood selenium concentration or gluthione peroxidase activity, and milk selenium concentrations in New Zealand dairy cows. N Z Vet J. Feb:49(1):24-8 https://doi.org/10.1080/00480169.2001.36198
  15. Grigor, P. N., Goddard, P. J., Littlewood, C. A. and Deakin, D. W. 1998. Pre-transport loading of farmed red deer: effects of previous overnight housing environment, vehicle illumination and shape of loading race. Vet Rec. 14;142(11): 265-268
  16. Hong, N. D., Won, J. H. and Kim, N. K. 1991. Studies on the analysis of constituents of deer Horn(I). kor. J. Pharmacogn. 22(3): 171-18
  17. Jia, X., Li, N. and Chen, J. 2005. A subchronic toxicity study of elemental Nano-Se in Sprague-Dawley rats. Life Sci. 76: 1989-2003 https://doi.org/10.1016/j.lfs.2004.09.026
  18. Kim, Y. Y. and Mahan, D. C. 2001. Prolonged feeding of high dietary levels of organic andinorganic selenium to gilts from 25kg body weight through one parity. J. Anim. Sci. 2001. 79:956-966
  19. Knowles, S. O., Grace, N. D., Knight, T. W., McNabb, W. C. and Lee, J. 2004. Adding nutritional value to meat and milk from pasture-fed livestock. N Z Vet J. Dec:52(6): 342-51 https://doi.org/10.1080/00480169.2004.36450
  20. Magg, D. D. and Glen, M. W. 1967. Toxicity of selenium: farm animals. Muth, O. H., Oldfield, J. E. and Heswig, P. H.(Eds.), Selenium in Biomedicine. Conn. AVI, Westport, pp. 127-140
  21. Moberg, G. P. and Mench, J. A. 2000. The biology of animal stress: Basic principles and implications for animal welfare. CABI Publishing, Wallingford, Oxon, U.K
  22. O'Toole, D. and Raisbeck, M. F. 1995. Pathology of experimentally induced chronic selenosis(alkali disease) in yearling cattle. J. Vet. Diagn. Invest. 7:364-373 https://doi.org/10.1177/104063879500700312
  23. Rayman, M. P. 2000. The importance of selenium to human health. The Lancet, 356:233-241 https://doi.org/10.1016/S0140-6736(00)02490-9
  24. Sakkinen, H., Tornberg, J., Goddard, P. J., Eloranta, E., Ropstad, E. and Saarela, S. 2004. The effect of blood sampling method on indicators of physiological stress in reindeer. Domestic Animal Endocrinology 26(2004) 87-98 https://doi.org/10.1016/j.domaniend.2003.07.002
  25. SAS. Institute, 1985. SAS$^{\circledR}$ User's guide: Statistics. Version 5 Ed. SAS institute Inc., Cary, NC.
  26. Schwarz, K. and Foltz, C. M. 1957. Se as an integral part of factor against dietary liver degeneration. J. Am. Chem. Society 79:3292-3296
  27. Wendelaar, S. E. and Balm, P. H. M. 1999. Histological and histopathlogical effects of stress. Page 182 in stress physiology in animals. P. H. M. Balm, ed. Sheffield Academic Press, Sheffield, U. K