Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Constituents of the seeds of Cornus officinalis with Inhibitory Activity on the Formation of Advanced Glycation End Products (AGEs)

산수유 씨의 최종당화산물 생성저해활성 성분

  • Lee, Ga-Young (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Jang, Dae-Sik (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Lee, Yun-Mi (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Young-Sook (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Jin-Sook (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine)
  • 이가영 (한국한의학연구원 한약제제연구부) ;
  • 장대식 (한국한의학연구원 한약제제연구부) ;
  • 이윤미 (한국한의학연구원 한약제제연구부) ;
  • 김영숙 (한국한의학연구원 한약제제연구부) ;
  • 김진숙 (한국한의학연구원 한약제제연구부)
  • Published : 2008.12.31
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Abstract

Ten compounds, (+)-pinoresinol (1), (-)-balanophonin (2), gallicin (3), vanillin (4), 4-hydroxybenzaldehyde (5), coniferaldehyde (6), betulinic acid (7), ursolic acid (8), 5-hydroxymethyl furfural (9), and malic acid (10), were isolated from a EtOAc-soluble fraction of the seeds of Cornus officinalis. The structures of these compounds were elucidated by spectroscopic methods as well as by comparison with reported values. Compounds 1, 2, and 4-7 were isolated from this species for the first time. All the isolates (1-10) were subjected to an in vitro bioassay to evaluate their inhibitory activity against advanced glycation end products (AGEs) formation. Among these, compounds 2 and 3 showed the significant inhibitory activity on AGEs formation with $IC_{50}$ values of 27.81 and 18.04${\mu}M$, respectively.

과육을 제거한 산수유 씨의 MeOH 추출물의 EtOAc 분획물로부터 이용하여 10종의 화합물을 분리하였다. 이들 중에서 (+)- pinoresinol, (-)-balanophonin, vanillin, 4-hydroxybenzaldehyde, coniferaldehyde, betulinic acid는 이 식물에서 처음으로 분리된 물질들이다. 본 연구에서 분리된 10종의 화합물에 대한 in vitro 최종당화산물(AGEs) 생성억제효능 실험결과, (-)-balanophonin (화합물 2)과 gallicin(화합물 3)이 각각 $IC_{50}$ 값 27.81과 18.04${\mu}M$로 비교양성대조군인 aminoguanidine(974.59 ${\mu}M$)보다 훨씬 우수한 효능을 나타내었다.

Keywords

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