Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Culture-Independent Analysis of Microbial Succession During Composting of Swine Slurry and Mushroom Cultural Wastes

  • Cho, Kye-Man (Research institute of Agriculture and Life Science) ;
  • Lee, Sun-Mi (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Math, Renukaradhya K. (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Islam, Shah Md. Asraful (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kambiranda, Devaiah M. (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Jong-Min (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Yun, Myoung-Geun (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Cho, Ji-Joong (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Kim, Jong-Ok (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Lee, Young-Han (Division of Plant Environmental Research, Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Kim, Hoon (Department of Bio-environmental Chemistry, Sunchon National University) ;
  • Yun, Han-Dae (Research institute of Agriculture and Life Science)
  • Published : 2008.12.31
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Abstract

Bacterial diversity and the composition of individual communities during the composting process of swine and mushroom cultural wastes in a field-scale composter (Hazaka system) were examined using a PCR-based approach. The composting process was divided into six stages based on recorded temperature changes. Phylogenetic analysis of eighty 16S rRNA sequences from uncultured composting bacterial groups revealed the presence of representatives from three divisions, including plant pathogenic bacteria, high-molecule-degrading bacteria and spore-forming bacteria. The plant pathogen A. tumefaciens gradually decreased in abundance during the composting process and eventually disappeared during the thermophilic and cooling stage. A bacterium homologous to Bacillus humi first appeared at the early thermophilic stage and was established at the intermediate thermophilic, post-thermophilic, and cooling stages. It was not possible to isolate the B. humi during any of the stages using general culture techniques.

Keywords

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