Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Psychrophile Shewanella sp. KMG427 as an Eicosapentaenoic Acid Producer

  • Lee, Won-Hae (Department of Microbiology, Kyungpook National University) ;
  • Cho, Ki-Woong (Department of Marine Biotechnology, Anyang University) ;
  • Park, Soo-Young (Department of Microbiology, Kyungpook National University) ;
  • Shin, Kee-Sun (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Dong-Sun (Department of Microbiology, Kyungpook National University) ;
  • Hwang, Seon-Kap (Department of Microbiology, Kyungpook National University) ;
  • Seo, Seok-Jong (Department of Microbiology, Kyungpook National University) ;
  • Kim, Jong-Myeong (Department of Microbiology, Kyungpook National University) ;
  • Ghim, Sa-Youl (Department of Microbiology, Kyungpook National University) ;
  • Song, Bang-Ho (Department of Biology Education, Kyungpook National University) ;
  • Lee, Sang-Han (Department of Food Science and Technology, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Microbiology, Kyungpook National University)
  • Published : 2008.12.31
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Abstract

An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at $4^{\circ}C$ after cell proliferation at $20^{\circ}C$. The isolated bacterium was categorized as Gram-negative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP $103207^T$. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.

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References

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