Food Science and Biotechnology

  • 제17권6호
  • /
  • Pages.1327-1331
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  • 2008
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  • 1226-7708(pISSN)
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  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Inhibitory Effect of Ethyl Acetate Extract of White Peach Pericarp on Adipogenesis of 3T3-L1 Preadipocyte Cells

  • Park, Hong-Gyu (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Kim, Jin-Moon (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Kim, Jung-Mogg (Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Chung, Won-Yoon (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Yoo, Yun-Jung (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Cha, Jeong-Heon (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry)
  • 발행 : 2008.12.31
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초록

In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor $\gamma$, CAAT enhancer binding protein $\alpha$, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.

키워드

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