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Inhibitory Effect of Ethyl Acetate Extract of White Peach Pericarp on Adipogenesis of 3T3-L1 Preadipocyte Cells  

Park, Hong-Gyu (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry)
Kim, Jin-Moon (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry)
Kim, Jung-Mogg (Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine)
Chung, Won-Yoon (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry)
Yoo, Yun-Jung (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry)
Cha, Jeong-Heon (Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University College of Dentistry)
Publication Information
Food Science and Biotechnology / v.17, no.6, 2008 , pp. 1327-1331 More about this Journal
Abstract
In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor $\gamma$, CAAT enhancer binding protein $\alpha$, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.
Keywords
Prunus persica; peach; obesity; C3H10T1/2; 3T3-L1;
Citations & Related Records
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Times Cited By Web Of Science : 0  (Related Records In Web of Science)
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