Antioxidant and Anti-inflammatory Activities of Equisetum hyemale

  • Park, Eun-Young (Department of Rehabilitation, College of Alternative Medicine, Jeonju University) ;
  • Jeon, Hoon (College of Pharmacy, Woosuk University)
  • 발행 : 2008.12.31

초록

Equisetum hyemale L. has been prescribed widely as a traditional medicine for the treatment of inflammatory diseases such as rheumatoid arthritis, conjunctivitis, pyelonephritis. In order to identification the mechanism, we examined an antioxidant and anti-inflammatory activity of 85% methanol extract of E. hyemale. In this study E. hyemale exhibited strong scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, superoxide radical, and nitric oxide. To elucidate the anti-inflammatory properties of E. hyemale, we investigated the inhibition effects of nitric oxide and IL-6 by E. hyemale in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. E. hyemale suppressed nitric oxide, IL-6 production and iNOS expression dose-dependently without notable cytotoxic activity. These data suggest that E. hyemale might be useful in inflammatory diseases by inhibiting the free radicals and inflammatory mediators.

키워드

참고문헌

  1. Bertolini, A., Ottani, A., and Sandrini, M., Dual acting anti-inflammatory drugs: a reappraisal. Pharmacol. Res. 44, 437-450 (2001) https://doi.org/10.1006/phrs.2001.0872
  2. Bonomini, F., Tengattini, S., Fabiano, A., Bianchi, R., and Rezzani, R., Atherosclerosis and oxidative stress. Histol Histopathol. 23, 381-390 (2008)
  3. Cao, X.Y., Dong, M., Shen, J.Z., Wu, B.B., Wu, P.M., Du, X.D., Wang, Z., Qi, Y.T., and Li, B.Y., Tilmicosin and tylosin have antiinflammatory properties via modulation of COX-2 and iNOS gene expression and production of cytokines in LPS-induced macrophages and monocytes. Int. J. Antimicrob. Agents 27, 431-438 (2006) https://doi.org/10.1016/j.ijantimicag.2005.12.010
  4. Chen, Y.J., Hsu, K.W., Tssai, J.N., Hung, C.H., Kuo, T.C., and Chen, Y.L., Involvement of protein kinase C in the inhibition of lipopolysaccharide- induced nitiric oxide production by thapsigargin in RAW 264.7 macrophages. Int. J. Biochem. Cell Biol. 37, 2574-2585 (2005) https://doi.org/10.1016/j.biocel.2005.07.002
  5. Conner, E.M. and Grisham, M.B., Inflammation, free radicals, and antioxidants. Nutrition. 12, 274-277 (1996) https://doi.org/10.1016/S0899-9007(96)00000-8
  6. D'Acquisto, F., May, M.J., and Ghosh, S., Inhibition of nuclear factor kappa B (NF-B): an emerging theme in anti-inflammatory therapies. Mol. Interv. 2, 22-35 (2002) https://doi.org/10.1124/mi.2.1.22
  7. D'Agostino, M., Dini, A., Pizza, C., Senatore, F., and Aquino, R., Sterols from Equisetum arvense. Boll-Soc. Ital. Biol. Sper. 60, 2241-2245 (1984)
  8. Do Monte, F.H., dos Santos, JG. Jr., Russi, M., Lanziotti, V.M., Leal, L.K., Cunha, GM., Antinociceptive and anti-inflammatory properties of the hydroalcoholic extract of stems from Equisetum arvense L. in mice. Pharmacol. Res. 49, 239-243 (2004) https://doi.org/10.1016/j.phrs.2003.10.002
  9. Erridge, C., Bennett-Guerrero, E., and Poxton, I.R., Structure and function of lipopolysaccharides. Microbes Infect. 4, 837-851 (2002) https://doi.org/10.1016/S1286-4579(02)01604-0
  10. Gyamfi, M.A., Yonamine, M., and Aniya, Y., Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen. Pharmacol. 32, 661-667 (1999) https://doi.org/10.1016/S0306-3623(98)00238-9
  11. Halliwell, B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence. Lancet. 344, 721-724 (1994) https://doi.org/10.1016/S0140-6736(94)92211-X
  12. Hewett, J.A. and Roth, R.A., Hepatic and extrahepatic pathobiology of bacterial lipopolysaccharides. Pharmacol. Rev. 45, 382-411 (1993)
  13. Ibrahim, H.R., Hoq, M.I., and Aoki, T., Ovotransferrin possesses SODlike superoxide anion scavenging activity that is promoted by copper and manganese binding. Int. J. Biol. Macromol. 41, 631-640 (2007) https://doi.org/10.1016/j.ijbiomac.2007.08.005
  14. Kaplan, M., Mutlu, E.A., Benson, M., Fields, J.Z., Banan, A., and Keshavarzian, A., Use of herbal preparations in the treatment of oxidant-mediated inflammatory disorders. Complement Ther. Med. 15, 207-216 (2007) https://doi.org/10.1016/j.ctim.2006.06.005
  15. Lee, Y.S., Han, O.K., Park, C.W., Yang, C.H., Jeon, T.W., Yoo, W.K., Kim, S.H., and Kim, H.J., Pro-inflammatory cytokine gene expression and nitric oxide regulation of aqueous extracted Astragali radix in RAW 264.7 macrophage cells. J. Ethnopharmacol. 100, 289-294 (2005) https://doi.org/10.1016/j.jep.2005.03.009
  16. Milovanovi , V., Radulovi , N., Todorovi , Z., Stankovi , M., and Stojanovi , G., Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. Plant Foods Hum. Nutr. 62, 113-119 (2007) https://doi.org/10.1007/s11130-007-0050-z
  17. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  18. Oh, H., Kim, D.H., Cho, J.H., and Kim, Y.C., Hepatoprotective and free radical scavenging activities of phenolic petrosins and flavonoids isolated from Equisetum arvense. J. Ethnopharmacol. 95, 421-424 (2004) https://doi.org/10.1016/j.jep.2004.08.015
  19. Oka, M., Tachibana, M., Noda, K., Inoue, N., Tanaka, M., and Kuwabara, K., Relevance of anti-reactive oxygen species activity to antiinflammatory activity of components of eviprostat, a phytotherapeutic agent for benign prostatic hyperplasia. Phytomedicine 14, 465-472 (2007) https://doi.org/10.1016/j.phymed.2007.04.006
  20. Park, S.J., Lee, S.C., Hong, S.H., and Kim, H.M., Degradation of IkappaBalpha in activated RAW264.7 cells is blocked by the phosphatidylinositol 3-kinase inhibitor LY294002. Cell Biol. Toxicol. 18, 121-130 (2002) https://doi.org/10.1023/A:1015384201785
  21. Rakel, D.P. and Rindfleisch, A., Inflammation: nutritional, botanical, and mind-body influences. South Med. J. 98, 303-310 (2005) https://doi.org/10.1097/01.SMJ.0000154775.16761.A9
  22. Saha, K., Lajis, N.H., Israf, D.A., Hamzah, A.S., Khozirah, S., Khamis, S., and Syahida, A., Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J. Ethnopharmacol. 92, 263-267 (2004) https://doi.org/10.1016/j.jep.2004.03.007
  23. Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Kwon, T.O., Yun, Y.G., Kim, N.Y., and Chung, H.T., Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide productions by murine macrophage cell line, RAW 264.7 cells. J. Ethnopharmacol. 76, 59-64 (2001) https://doi.org/10.1016/S0378-8741(01)00221-5
  24. Syrchina, A.I., Voronkov, M.G., Tyukavkina, N.A., and Babkin, V.A., Phenolic acids and flavonoids of sporogenous stems of Equisetum arvense. Khim. Prir. Soedin. 6, 803-804 (1978)
  25. Syrchina, A.I., Zapesochnaya, G.G., Tyukavkina, N.A., and Voronkov, M.G., Glycosides of Equisetum arvense flavones. Khim. Prir. Soedin. 3, 413-414 (1980)
  26. Takatsuto, S., Abe, H., and Gamoh, K., Evidence for brassino-steroids of Equisetum arvense L. Agric. Biol. Chem. 54, 1057-1059 (1990)
  27. Thiemermann, C. and Vane, J., Inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharides in the rat in vivo. Eur. J. Pharmacol. 182, 591-595 (1990) https://doi.org/10.1016/0014-2999(90)90062-B
  28. Watt, J.G., The price of factor VIII. Lancet. 2, 301 (1979)
  29. Xu, C.F., Bian, X.Y., Qu, S.M., You, L.H., Qi, Z.M., Cheng, W., Liu, X.J., Liu, W.Z., and Ren, S.J., Effect of Equisetum hyemale on experimental hyperlipemia in rats and its toxic test. Zhongguo Zhong Yao Za Zhi 18, 52-53 (1993)