The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
권호 표지

Detection of periodontal disease related bacteria from the implant-abutment interface in oral cavity

구강내 임플랜트-지대주 연결부에서 치주질환관련 세균의 검출

  • Han, Meung-Ju (Department of Prosthodontics, College of Dentistry, Chosun University) ;
  • Chung, Chae-Heon (Department of Prosthodontics, College of Dentistry, Chosun University) ;
  • Kim, Hee-Jung (Department of Prosthodontics, College of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Departtment of Biochemistry, College of Dentistry, Chosun University) ;
  • Yoo, So-Young (Departtment of Biochemistry, College of Dentistry, Chosun University)
  • 한명주 (조선대학교 치과대학 치과보철학교실) ;
  • 정재헌 (조선대학교 치과대학 치과보철학교실) ;
  • 김희중 (조선대학교 치과대학 치과보철학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실) ;
  • 유소영 (조선대학교 치과대학 구강생화학교실)
  • Published : 2008.04.30
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Abstract

Statement of the problem: Implant systems result in gaps and cavities between implant and abutment that can act as a trap for bacteria and thus possibly cause inflammatory reactions in the peri-implant soft tissues. Purpose: Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, and Aggregatibacter actinomycetemcomitans, related to implant-abutment interface microleakage. Material and methods: Samples were taken from 27 subjects with sterilized paper points and were transported in $1{\times}PBS$. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA. Results: Our data showed that the detection rate of P. gingivalis and P. intermedia in implant fixture was 59% and 82% in patients respectively. Detection rate of P. gingivalis and P. intermedia in implant crevice was 44% and 82% in patients. Detection rate of P. gingivalis and P. intermedias in tongue was 82% and 82% in patients. Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.

문제 진술: 임플랜트 시스템은 임플랜트와 지대주 사이에서 빈 공간이 발생하여 세균의 저장소로서 작용하게 되어 임플랜트 주위 연조직에 염증반응을 야기할 수 있다. 목 적: 이 연구의 목적은 임플랜트-지대주 간극의 미세 누출에 관여하는 치주질환원인균으로 추정되는 Porpyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans의 검출률을 조사하는 것이다. 연구재료 및 방법: 시료채취는 27명의 환자들에서 행하였고 소독된 페이퍼포인트를 이용하여 $1{\times}PBS$로 옮겼다. 치주질환원인균의 검출은 16 rDNA에 근거한 종특이적인 프라이머를 지닌 중합효소체인반응을 이용하였다. 결과: 임플랜트 고정체내에서의 Porphyromonas gingivalis와 Prevotella intermedia의 검출률은 59%와 82%였다. 환자의 임플랜트 연구에서는 Porphyromonas gingivalis와 Prevotella intermedia의 검출률은 44%와 82%였다. 환자혀에서의 Porphyromonas gingivalis와 Prevotella intermedia의 검출률은 82%와 82%였다. 결 론: 현재의 임플랜트 시스템은 임플랜트 내부의 세균 군집화와 미세누출을 안전하게 예방할 수 없다.

Keywords

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