Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Surface Plasmon Resonance Immunosensor through Metal Ion Affinity and Mixed Self-Assembled Monolayer

  • Lee, Si-Ra (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Sim, Sang-Jun (Department of Chemical Engineering, Kwangwoon University) ;
  • Park, Chul-Hwan (College of Life Sciences and Biotechnology, Korea University) ;
  • Gu, Man-Bock (Department of Bio-Chemical Engineering, Dongyang University) ;
  • Hwang, Un-Yeon (School of Chemical and Biological Engineering, Seoul National University) ;
  • Yi, Jong-Heop (Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • Oh, Byung-Keun (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Published : 2008.10.31
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Abstract

An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16-mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of $Zn(NO_3)_2\cdot 6H_2O$. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphate-buffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.

Keywords

References

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