Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Liposome Immunoassay for Salmonella spp. using Immunomagnetic Separation and Immunoliposome

  • Shin, Jung-Hee (School of Food Science/Technology and Food Service Industry, College of Natural Resources, Yeungnam University) ;
  • Kim, Myung-Hee (School of Food Science/Technology and Food Service Industry, College of Natural Resources, Yeungnam University)
  • Published : 2008.10.31
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Abstract

The ability to detect Salmonella spp. is essential in the prevention of foodborne illness. This study examined a Salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (IMS/IL) encapsulating sulforhodamine B (SRB), a fluorescent dye. A quantitative assay was conducted by measuring the fluorescence intensity of SRB that was produced from an immunomagnetic bead-Salmonella spp.-immunoliposome complex. The results indicated detection limits of $2.7{\times}10^{5}$ and $5.2{\times}10^{3}$ CFU/ml for Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterka subsp. enterka serovar Typhimurium (S. Typhimurium), respectivley. The signal/noise ratio was improved by using 4% skim milk as a wash solution rather than 2% BSA. In addition, higher fluorescence intensity was obtained by increasing the liposome size. Compared with the conventional plating method, which takes 3-4 days for the isolation and identification of Salmonella spp., the total assay time of to h only including 6 h of culture enrichment was necessary for the Salmonella detection by IMS/IL. These results indicate that the IMS/ IL has great potential as an alternative rapid method for Salmonella detection.

Keywords

References

  1. Bangham, A. D., M. M. Standish, and J. C. Watkins. 1965. Diffusion of univalent ions across the lamellae of swollen phospholipids. J. Mol. Biol. 13: 238-252 https://doi.org/10.1016/S0022-2836(65)80093-6
  2. Bautista, D. A., S. Elankumaran, J. A. Arking, and R. A. Heckert. 2002. Evaluation of an immunochromatography strip assay for the detection of Salmonella sp. from poultry. J. Vet. Diagn. Invest. 14: 427-430 https://doi.org/10.1177/104063870201400514
  3. Chen, L., L. Deng, L. Liu, and Z. Peng. 2007. Immunomagnetic separation and MS/SPR end-detection combined procedure for rapid detection of Staphylococcus aureus and protein A. Biosens. Bioelectron. 22: 1487-1492 https://doi.org/10.1016/j.bios.2006.06.038
  4. Cudjoe, K. S., L. I. Thorsen, T. Sorensen, J. Reseland, O. Olsvik, and P. E. Granum. 1991. Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA. Int. J. Food Microbiol. 12: 313-321 https://doi.org/10.1016/0168-1605(91)90145-F
  5. Davies, C. 1994. Technical performance concepts. In D. Wild (ed.), The Immunoassay Handbook. Macmillan Press, London
  6. DeCory, T. R., R. A. Durst, S. J. Zimmerman, L. A. Garringer, G. Paluca, H. H. DeCory, and R. A. Montagna. 2005. Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method. Appl. Environ. Microbiol. 71: 1856-1864 https://doi.org/10.1128/AEM.71.4.1856-1864.2005
  7. Ellingson, J. L. E., J. L. Anderson, S. A. Carlson, and V. K. Sharma. 2004. Twelve hour real-time PCR technique for the sensitive and specific detection of Salmonella in raw and ready-to-eat meat products. Mol. Cell. Probes 18: 51-57 https://doi.org/10.1016/j.mcp.2003.09.007
  8. Favrin, S. J., S. A. Jassim, and M. W. Griffiths. 2003. Application of a novel immunomagnetic separation-bacteriophage assay for the detection of Salmonella Enteritidis and Escherichia coli O157:H7 in food. Int. J. Food Microbiol. 85: 63-71 https://doi.org/10.1016/S0168-1605(02)00483-X
  9. Fu, Z., S. Rogelj, and T. L. Kieft. 2005. Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR. Int. J. Food Microbiol. 99: 47-57 https://doi.org/10.1016/j.ijfoodmicro.2004.07.013
  10. Furuya, Y., S. Cho, S. Ohta, N. Sato, T. Kotake, and M. Masai. 2001. High dose hook effect in serum total and free prostate specific antigen in a patient with metastatic prostate cancer. J. Urol. 166: 213 https://doi.org/10.1016/S0022-5347(05)66117-4
  11. Gessler, F., K. Hampe, M. Schmidt, and H. Böhnel. 2006. Immunomagnetic beads assay for the detection of botulinum neurotoxin types C and D. Diagn. Microbiol. Infect. Dis. 56: 225-232 https://doi.org/10.1016/j.diagmicrobio.2006.04.014
  12. Grant, I. R., H. J. Ball, and M. T. Rowe. 1998. Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation. Appl. Environ. Microbiol. 64: 3153-3158
  13. Hibi, K., A. Abe, E. Ohashi, K. Mitsubayashi, H. Ushio, T. Hayashi, H. Ren, and H. Endo. 2006. Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes. Anal. Chim. Acta 573-574: 158-163 https://doi.org/10.1016/j.aca.2006.03.022
  14. Jung, S. J., H. J. Kim, and H. Y. Kim. 2005. Quantitative detection of Salmonella Typhimurium contamination in milk, using real-time PCR. J. Microbiol. Biotechnol. 15: 1353-1358
  15. Korea Food and Drug Administration. 2005. Food Code. Korea Food and Drug Administration, Seoul
  16. Kumar, S., K. Balakrishna, G. P. Singh, and H. V. Batra. 2005. Rapid detection of Salmonella Typhi in foods by combination of immunomagnetic separation and polymerase chain reaction. World J. Microbiol. Biotechnol. 21: 625-628 https://doi.org/10.1007/s11274-004-3553-x
  17. Lamoureux, M., A. MacKay, S. Messier, I. Fliss, B. W. Blais, R. A. Holley, and R. E. Simard. 1997. Detection of Campylobacter jejuni in food and poultry viscera using immunomagnetic separation and microtitre hybridization. J. Appl. Microbiol. 83: 641-651 https://doi.org/10.1046/j.1365-2672.1997.00273.x
  18. Lee, H. A., G. M. Wyatt, S. Bramham, and M. R. A. Morgan. 1990. Enzyme-linked immunosorbent assay for Salmonella Typhimurium in food: Feasibility of 1-day Salmonella detection. Appl. Environ. Microbiol. 56: 1541-1546
  19. LeJeune, J. T., D. D. Hancock, and T. E. Besser. 2006. Sensitivity of Escherichia coli O157 detection in bovine feces assessed by broth enrichment followed by immunomagnetic separation and direct plating methodologies. J. Clin. Microbiol. 44: 872-875 https://doi.org/10.1128/JCM.44.3.872-875.2006
  20. Liu, Y. and Y. Li. 2002. Detection of Escherichia coli O157:H7 using immunomagnetic separation and absorbance measurement. J. Microbiol. Methods 51: 369-377 https://doi.org/10.1016/S0167-7012(02)00107-0
  21. Locascio-Brown, L., A. L. Plant, V. Horváth, and R. A. Durst. 1990. Liposome flow injection immunoassay: Implication for sensitivity, dynamic range, and antibody regeneration. Anal. Chem. 62: 2587-2593 https://doi.org/10.1021/ac00222a013
  22. Mansfield, L. and S. Forsythe. 1996. Collaborative ring-trial of $Dynabeads^{\circledR}$ anti-Salmonella for immunomagnetic separation of stressed Salmonella cells from herbs and spices. Int. J. Food Microbiol. 29: 41-47 https://doi.org/10.1016/0168-1605(95)00019-4
  23. Mercanoglu, B., S. A. Aytac, M. A. Ergun, and E. Tan. 2003. Isolation of Listeria monocytogenes by immunomagnetic separation and atomic force microscopy. J. Microbiol. 41: 144-147
  24. Ogden, I. D., M. MacRae, N. F. Hepburn, and N. J. C. Strachan. 2000. Improved isolation of Escherichia coli O157 using large enrichment volumes for immunomagnetic separation. Lett. Appl. Microbiol. 31: 338-341 https://doi.org/10.1046/j.1472-765x.2000.00827.x
  25. Olsvik, O., T. Popovic, E. Skjerve, K. S. Cudjoe, E. Hornes, J. Ugelstad, and M. Uhlen. 1994. Magnetic separation techniques in diagnostic microbiology. Clin. Microbiol. Rev. 7: 43-54 https://doi.org/10.1128/CMR.7.1.43
  26. Rijpens, N., L. Herman, F. Vereecken, G. Jannes, J. D. Smedt, and L. D. Zutter. 1999. Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR. Int. J. Food Microbiol. 46: 37-44 https://doi.org/10.1016/S0168-1605(98)00171-8
  27. Rongen, H. A. H., A. Bult, and W. P. van Bennekom. 1997. Liposomes and immunoassays. J. Immunol. Methods 204: 105-133 https://doi.org/10.1016/S0022-1759(97)00041-0
  28. Rule, G. S., D. A. Palmer, S. G. Reeves, and R. A. Durst. 1994. Use of protein A in a liposome-enhanced flow-injection immunoassay. Anal. Proceed. Incl. Anal. Commun. 31: 339-340 https://doi.org/10.1039/ai9943100339
  29. Shin, W. S., N. S. Kim, H. S. Yoon, and H. J. Lee. 2005. Novel immunoassay for Salmonella spp. using nanoliposome, pp. 5-11. Final Report. ARPC, Seoul
  30. Szoka, F. Jr. and D. Papahadjopoulos. 1978. Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse evaporation. Proc. Natl. Acad. Sci. USA 75: 4194-4198
  31. Vermunt, A. E. M., A. A. J. M. Franken, and R. R. Beumer. 1992. Isolation of salmonellas by immunomagnetic separation. J. Appl. Bacteriol. 72: 112-118 https://doi.org/10.1111/j.1365-2672.1992.tb01811.x