Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Antioxidant potentials of Hypericum hookerianum (Family: Hypericaceae) on CCl4 induced hepatotoxicity in rats

  • Wahile, Atul (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Mukherjee, Kakali (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Kumar, Venkatesan (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Saha, Bishnu Pada (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Mukherjee, Pulok K (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University)
  • Published : 2007.03.31
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Abstract

Free radicals are known to play important role in pathophysiology of hepatic disorders and antioxidants are employed along with other chemotherapeutic agents in treatment of such diseases. In search of natural antioxidant, successive extracts of Hypericum (H.) hookerianum (Family: Hypericaceae) were evaluated by in vitro and in vivo methods. Extracts of aerial parts of H. hookerianum were subjected for 1,1-diphenyl 2-picryl hydrazyl radical scavenging activity (DPPH assay), nitric oxide radicals scavenging assay and thiobarbituric acid reactive substances (TBARS) assay. Methanolic extract was found to be more active than other extracts in DPPH and in vitro TBARS assay with $IC_{50}$ at 5.82 ${\pm}$ 1.33 ${\mu}g/ml$ and 49.78 ${\pm}$ 3.79 ${\mu}g/ml$ respectively. While petroleum ether extract showed more potentials in scavenging the nitric oxide radicals with $IC_{50}$ 220.97 ${\pm}$ 2.69 ${\mu}g/ml$. The administration of $CCl_{4}$ to the control animals caused decrease in the level of catalase and superoxide dismutase, together with significant increase in the level of TBARS in liver and kidney. Reversal of these changes towards normal group was observed by administration of H. hookerianum methanolic extract at 50 and 100 mg/kg body weight, while other extracts were found to be less active.

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References

  1. Ajitha M, Rajnarayana K. (2001) Role of oxygen free radicals in human diseases. Indian Drugs 38, 545-554
  2. Anila L, Vijayalakshmi NR. (2003) Antioxidant action of flavonoids from Mangifera indica and Emblica officinalis in hypercholesterolemic rats. Food Chem. 83, 569-574 https://doi.org/10.1016/S0308-8146(03)00155-9
  3. Anonymous. (1997) The Wealth of India, Vol. V, p. 154, PID CSIR, New Delhi.
  4. Badami S, Moorkoth S, Rai SR, Karman E, Bhojraj 5. (2003) Antioxidant activity of Caesalpinia sappan heartwood. Biol. Pharm. Bull. 26, 1534-1537 https://doi.org/10.1248/bpb.26.1534
  5. Bardhan P, Sharma SK, Garg NK. (1985) In vitro effect of an Ayurvedic liver remedy on hepatic enzymes in carbon tetrachloride treated rats. Indian J. Med. Res. 82, 359-364
  6. Beer RF Jr, Seizer TW. (1952) A spectrophotometric method for measuring breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 115, 130-140
  7. Benedi J, Arroyo R, Romero C, Martin-Aragon S, Villar AM. (2001) Antioxidant properties and protective effects of a standardized extract of Hypericum petforatum on hydrogen peroxide-induced oxidative damage in PC12 cells. Life Sci. 75, 1263-1276 https://doi.org/10.1016/j.lfs.2004.05.001
  8. Bhattacharya A, Ghosal S, Bhattacharya SK. (2001) Anti-oxidant effect of Withania somnifera glycowithanolides in chronic footshock stress-induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum. J. Ethnopharmacol. 74, 1-6 https://doi.org/10.1016/S0378-8741(00)00309-3
  9. Cakir A, Mavi A, Yildirim A, Durn ME, Harmandar M, Kazaz C (2003) Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L. by activity-guided fractionation. J. Ethnopharmacol. 87, 73-83 https://doi.org/10.1016/S0378-8741(03)00112-0
  10. Cheeseman KH, Seater TF. (1993) Free radical in medicine. Br. Med. Bull. 49, 479-724
  11. Conforti F, Statti GA, Tundis R Menichini F, Houghton P. (2002) Antioxidant activity of methanolic extract of Hypericum triquetrifolium Turra aerial part. Fitoterapia 73, 479-483 https://doi.org/10.1016/S0367-326X(02)00162-4
  12. Couladis M, Baziou P, Verykokidou E, Loukis A. (2002) Antioxidant Activity of Polyphenols from Hypericum triquetrifolium Turra. Phytother. Res. 16, 769-770 https://doi.org/10.1002/ptr.1062
  13. El-Sherbiny DA, Khalifa AE, Attia AS, Eldenshary Eel-D. (2003) Hypericum perforatum extract demonstrates antioxidant properties against elevated rat brain oxidative status induced by amnestic dose of scopolamine. Pharmacol. Biochem. Behan. 76, 525-533 https://doi.org/10.1016/j.pbb.2003.09.014
  14. Gamez EJ, Luyengi L, Lee SK, Zhu LF, Zhou BN, Fong HH, Pezzuto JM, Kinghorn AD. (1998) Antioxidant flavonoid glycosides from Daphniphyllum calycinum. J. Nat. Prod. 61, 706-708 https://doi.org/10.1021/np9800203
  15. Garrat DC (1964) The Quantitative Analysis of Drugs, Vol. 3, pp. 456-458, Chapman and Hall Ltd., Japan
  16. Gutteridge JM. (1993) Free radicals in disease processes: a compilation of cause and consequence. Free Radic. Res. Commun. 19, 141-158
  17. Halliwell B, Gutteridge JMC, (1989) Free Radicals in Biology and Medicine. 2nd ed., Clarendron Press, Oxford
  18. Halliwell B. (1990) How to characterize a biological antioxidant. Free Radic. Res. Commun. 9, 1-32
  19. Halliwell B, Gutteridge JM, Cross CE. (1992) Free radicals, antioxidants and human diseases: where are we now? J. Lab. Clin. Med. 119, 598-613
  20. Halliwell B, Gutteridge JMC (1995) Free Radicals in Biology and Medicine. Oxford: Clarendon Press
  21. Hunt EJ, Lester CE, Lester EA. (2001) Effect of St. John's wort on free radical production. Life Sci. 69, 181-190 https://doi.org/10.1016/S0024-3205(01)01102-X
  22. Hwang BY, Kim HS, Lee JH, Hong YS, Ro JS, Lee KS, Lee JJ. (2001) Antioxidant benzoylated flavan-3-ol glycoside from Celastrus orbiculatus. J. Nat. Prod. 64, 82-84 https://doi.org/10.1021/np000251l
  23. Kaplowitz N, Aw IT, Simon FR, Stolz A. (1986) Drug-induced hepatotoxicity. Ann. Intern. Med. 104, 826-839
  24. Marcocci L, Packer L, Droy-Lefaix MT, Sekaki A, Gardes-Albert M. (1994) Antioxidant action of Ginkgo biloba extract EGb 761. Meth. Enzymol. 234, 462-475 https://doi.org/10.1016/0076-6879(94)34117-6
  25. Maurizio P, Leonarduzzi G, Biasi F, Albano E, Biocca ME, Poli G, Dianzani MU. (1992) Vitamin E dietary supplementation protects against carbon tetrachloride-induced chronic liver damage and cirrhosis. Hepatology 16, 1014-1021 https://doi.org/10.1002/hep.1840160426
  26. Misra HP, Fridovich I. (1972) The role of superoxide anion in the antioxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247, 3170-3175
  27. Mukherjee PK, Suresh B. (2000) The evaluation of wound-healing potential of Hypericum hockerianum leaf and stem extracts. J. Altern. Complement. Med. 6, 61-69 https://doi.org/10.1089/acm.2000.6.61
  28. Mukherjee PK, Saritha GS, Suresh B. (2001a) Antibacterial spectrum of Hypericum hookerianum. Fitoterapia 72, 558-560 https://doi.org/10.1016/S0367-326X(00)00331-2
  29. Mukherjee PK, Suresh B, Verpoorte R. (2001b) CNS active potentials of some Hypericum species of India. Phytomedicine 8, 331-337 https://doi.org/10.1078/0944-7113-00054
  30. Mukherjee PK, Wahile A, Nazeer A, Rajan S. (2003) Hypericum Source of Natural Antimicrobials. Orient. Pharm. Exp. Med. 3, 111-122
  31. Mukherjee PK. (2002) Quality Control of Herbal Drugs - An Approach to Evaluation of Botanicals, pp. 560-567, Business Horizons, New Delhi, India
  32. Mukherjee PK. (2003) Plant products with hypocholesterolemic potentials. Adv. Food Nutr. Res. 47, 278-324
  33. Mukherjee PK, Verpoorte R. (2003) GMP in Herbal Drugs, pp 366-367, Eastern Publishers, Business Horizons Ltd, New Delhi, India
  34. Ohkawa H, Ohishi N, Yagi K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  35. Rai S, Wahile A, Mukherjee K, Saha BP, Mukherjee PK. (2006) Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. J. Ethnopharmacol. 104, 322-327 https://doi.org/10.1016/j.jep.2005.09.025
  36. Raja S, Nazeer Ahamed KFH, Kumar V, Mukherjee K, Bandyopadhyay A, Mukherjee PK. (2005a) Antioxidant potential of Aerial part of Asclepias curassavica. Linn (Family - Asclepiadaceae). Orient. Pharm. Exp. Med. 5, 92-99
  37. Raja S, Nazeer Ahamed KFH, Kumar V, Mukherjee K, Bandyopadhyay A, Mukherjee PK (2005b) Exploring the Effect of Asclepias curassavica on Markers of Oxidative Stress in Rats. Evidence Based Integr. Med. 2, 87-93 https://doi.org/10.2165/01197065-200502020-00004
  38. Raja S, Ahamed KF, Kumar V, Mukherjee K, Bandyopadhyay A, Mukherjee PK (2006) Antioxidant effect of Cytisus scoparius against carbon tetrachloride treated liver injury in rats. J. Ethnopharmacol. (In press)
  39. Rajlakshmi D, Banerjee SK, Sood S, Maulik SK (2003) In-vitro and in-vitro antioxidant activity of different extracts of the leaves of Clerodendron colebrookianum Walp in rats. J. Pharm. Pharmacol. 55, 1681-1686 https://doi.org/10.1211/0022357022296
  40. Rohini G, Sabitha KE, Devi CS. (2004) Bacopa monniera Linn. extract modulates antioxidant and marker enzyme status in fibrosarcoma bearing rats. Indian J. Exp. Biol. 42, 776-780
  41. Saxena A, Garg NK (1979) Effect of Liv-52 on hepatic enzymes. Indian J. Exp. Biol. 17, 662-664
  42. Saxena A, Garg NK (1981) Effect of Liv-52 on membrane lipids in carbon tetrachloride induced hepatotoxicity in rats. Indian J. Exp. Biol. 19, 859-862
  43. Sharma MK, Buettner GR. (1993) Interaction of vitamin C and vitamin E during free radical stress in plasma: an ESR study. J. Free Radic. Biol. Med. 14, 649-653 https://doi.org/10.1016/0891-5849(93)90146-L
  44. Sundararajan R, Haja NA, Venkatesan K, Mukherjee K, Saha BP, Bandyopadhyay A, Mukherjee PK (2006) Cytisus scoparius link-a natural antioxidant. BMC Complement. Altern. Med. 16, 6 (In press)
  45. Trocino RA, Akazawa S, Ishibashi M, Matsumoto K, Matsuo H, Yamamoto H, Goto S, Urata Y, Kondo T, Nagataki S. (1995) Significance of glutathione depletion and oxidative stress in early embryogenesis in glucose-induced rat embryo culture. Diabetes 44, 992-998 https://doi.org/10.2337/diabetes.44.8.992
  46. Upton. (1997) St. John's Wort, American Herbal Pharmacopoeia and Therapeutic Compendium, p.12, Pittsburgh, PA, U.S.A.
  47. Visioli F, Keaney JF, Halliwell B. (2000) Antioxidants and cardiovascular disease; panaceas or tonics for tired sheep? Cardiovasc. Res. 47, 409 https://doi.org/10.1016/S0008-6363(00)00156-5
  48. Yagi K, Rastogi R. (1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95, 351 https://doi.org/10.1016/0003-2697(79)90738-3