Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Kinetics and diffusion studies in urease-alginate biocatalyst beads

  • Nakarani, Mukesh (Department of Chemical Engineering & Technology, Institute of Technology, Banaras Hindu University) ;
  • Kayastha, Arvind M (School of Biotechnology, Faculty of Science, Banaras Hindu University)
  • Published : 2007.03.31
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Abstract

Urease was immobilized with calcium alginate by entrapment method in the form of spherical beads and stored in Tris/acetate buffer (pH 7.3) at $4^{\circ}C$. Urease immobilized at different concentration of alginate beads (3%, 4% and 5%) showed higher apparent $K_{m}$ values than the soluble urease. Furthermore, $K_{m}$ has been shown to be corelated with effective diffusion coefficient (De) at different concentration of alginate gel. The present study showed that diffusion and reaction contribute to control the overall rate.

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References

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