Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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The Estrogenic Effects of Phthalates (DEHP, DBP) in MCF-7 Cell

유방암세포인 MCF-7세포를 이용한 DEHP, DBP의 에스트로젠 효과

  • Lee, Su-Youn (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, So-Jung (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Seung-Ho (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Young-Seok (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Sang-Ki (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Choi, Chang-Sun (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Yoon, Seong-Il (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Kim, Jong-Suk (Department of Biochemistry, Institute of Medical Science, Chunbuk National University Medical School) ;
  • Jung, Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
  • 이수연 (공주대학교 특수동물학과) ;
  • 김소정 (공주대학교 특수동물학과) ;
  • 이성호 (공주대학교 특수동물학과) ;
  • 박영석 (공주대학교 특수동물학과) ;
  • 박병권 (공주대학교 특수동물학과) ;
  • 김병수 (공주대학교 특수동물학과) ;
  • 김상기 (공주대학교 특수동물학과) ;
  • 최창순 (중앙대학교 식품영양학과) ;
  • 윤성일 (한양대학교 생명과학과) ;
  • 김종석 (전북대학교 의과대학 생화학교실) ;
  • 정지원 (서울대학교 수의학과) ;
  • 정지윤 (공주대학교 특수동물학과)
  • Published : 2007.09.30
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Abstract

To evaluate the estrogenic activities of di-ethyl hexyl phthalate (DEHP) and di-butyl phthalate (DBP), two phthalates known as endocrine disrupters, we used MCF-7 human breast cancer cell line. As results, DBP and DEHP had estrogenic effects. In brief, the concentration of maximal MCF-7 cell proliferation was $10^{-7}M\;and\;10^{-8}M$ for DEHP and DBP, respectively. The ratio of maximal cell yield of the test compounds to that of $17{\beta}-estradiol$ was 87.5% for DEHP and 73.4% for DBP. In summary, both DEHP and DBP had cell proliferation potencies in the MCF-7 cell. Potencies ranged from approximately 10 to 100 times less than 17beta-estradiol. DBP was stronger than DEHP in the concentration of maximal efficacy. However, DEHP was stronger than DBP in the MCF-7 cell proliferation. Results from this study suggested that DEHP and DBP may play an important role in the estrogenic activity. Therefore, it is suggested that DEHP and DBP are estrogenic.

내분비 교란 물질 중에서 최근에 문제가 되고 있는 물질이 프탈레이트계통 물질로서 플라스틱의 가소제로 사용하고 있는 DEHP와 DBP에 대하여 사람유방암세포 이면서 에스트로젠 의존성을 가지고 있는 MCF-7 세포에서의 세포 증식 정도를 농도 별로 측정하여 두 물질의 에스트로젠 작용가능성에 대하여 조사하였다. 시험물질인 DEHP와 DBP의 MCF-7 세포의 최대 증식 시의 농도를 조사해 본 결과, DEHP는 $17{\beta}-Estradiol$에 비하여 100배정도 높은 농도인 $10^{-7}M$ 에서 최대 증식능력을 보였고, DBP는 10배정도 높은 $10^{-8}M$ 에서 최대 증식능력을 보였다. 최대 증식 능력을 보일 때의 양성대조물질인 $17{\beta}-Estradiol$와 증식 정도 차이를 비교하였을 때에는 DEHP는 양성대조군 대비 87.5%의 증식 정도를 나타내었고, DBP는 73.4%의 증식 정도를 나타내었다 결론적으로 DEHP와 DBP 두 물질 모두 MCF-7 세포의 증식에 영향을 주는 것으로 판단되었으며, 최대 작용농도에 있어서는 DBP>DEHP, 세포 증식 정도에 있어서는 DEHP>DBP 인 것으로 판단되어 진다.

Keywords

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