Animal cells and systems

  • 제11권1호
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  • Pages.23-31
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  • 2007
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Characterization of Programmed Cell Death in the Silkworm Thoracic Ganglia during Postembryonic Periods

  • Kim, Soon-Ok (School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Mi-Young (School of Life Sciences and Biotechnology, Korea University) ;
  • Song, Hwa-Young (School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Jin-Hee (School of Life Sciences and Biotechnology, Korea University) ;
  • Kang, Pil-Don (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Lee, Bong-Hee (School of Life Sciences and Biotechnology, Korea University)
  • 발행 : 2007.06.30
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초록

Programmed cell death was characterized in the silkworm thoracic ganglia TG1, TG2 and TG3 during postembryonic periods by TUNEL assay. Apoptotic cells were detected in the three TGs of all larval stages except for day-1, 2 1st instar larvae, in which no apoptotic cells were found. From day-7 5th larva, the numbers of apoptotic cells were dramatically increased and peaked on day-1 pupa and day-2 pupa and then abruptly decreased. Apoptotic cells finally disappeared in day-1 adult. In-vivo injection of 20-hydroxyecdysone (20E) into day-8 5th larva resulted in a striking decrease of apoptotic cells. Actinomycin D (Act D) or cycloheximide (CHX), injected into hemolymph of day-8 5th larva, resulted in a decrease of apoptotic cells in the three TGs. Injection of caspase-8 and -3 inhibitors also blocked cellular apoptosis. These results will provide valuable information for understanding of cellular changes in the three TGs during metamorphosis of the insect species.

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참고문헌

  1. Aravind L, Dixit VM and Koonin EV (1999) The domains of death: evolution of the apoptosis machinery. Trends Biochem Sci 24: 47-53 https://doi.org/10.1016/S0968-0004(98)01341-3
  2. Blaschke AJ, Weiner JA and Chun J (1998) Programmed cell death is a universal feature of embryonic and postnatal neuroproliferative regions throughout the central nervous system. J Comp Neurol 396: 39 https://doi.org/10.1002/(SICI)1096-9861(19980622)396:1<39::AID-CNE4>3.0.CO;2-J
  3. Boatright KM, Renatus M, Scott FL, Sperandio S, Shin H, Pedersen IM, Ricci JE, Edris WA, Sutherlin DP, Green DR and Salvesen GS (2003) A unified model for apical caspase activation. Mol Cell 11: 529-541 https://doi.org/10.1016/S1097-2765(03)00051-0
  4. Bossy-Wetzel E and Green DR (2000) Detection of apoptosis by annexin V labeling. Methods Enzymol 322: 15-18 https://doi.org/10.1016/S0076-6879(00)22004-1
  5. Breedlove SM (1992) Sexual dimorphism in the vertebrate nervous system. J Neurosci 12: 4133-4142 https://doi.org/10.1523/JNEUROSCI.12-11-04133.1992
  6. Burek MJ and Oppenheim RW (1996) Programmed cell death in the developing nervous system. Brain Pathol 6: 427-446 https://doi.org/10.1111/j.1750-3639.1996.tb00874.x
  7. Caldero J, Prevette D, Mei X, Oakley RA, Li L, Milligan C, Houenou L, Burek M and Oppenheim RW (1998) Peripheral target regulation of the development and survival of spinal sensory and motor neurons in the chick embryo. J Neurosci 18: 356-370 https://doi.org/10.1523/JNEUROSCI.18-01-00356.1998
  8. Carr VM and Simpson SB, Jr. (1981) Rapid appearance of labeled degenerating cells in the dorsal root ganglia after exposure of chick embryos to tritiated thymidine. Brain Res 254: 157-162
  9. Champlin DT and Truman JW (1998) Ecdysteroid control of cell proliferation during optic lobe neurogenesis in the moth Manduca sexta. Development 125: 269-277
  10. Clem RJ and Miller LK (1994) Control of programmed cell death by the baculovirus genes p35 and iap. Mol Cell Biol 14: 5212- 5222 https://doi.org/10.1128/MCB.14.8.5212
  11. D'Mello SR (1998) Molecular regulation of neuronal apoptosis. Curr Top Dev Biol 39: 187-213 https://doi.org/10.1016/S0070-2153(08)60456-1
  12. Diaz B, Pimentel B, de Pablo F and de La Rosa EJ (1999) Apoptotic cell death of proliferating neuroepithelial cells in the embryonic retina is prevented by insulin. Eur J Neurosci 11: 1624-1632 https://doi.org/10.1046/j.1460-9568.1999.00577.x
  13. Dorstyn L, Colussi PA, Quinn LM, Richardson H and Kumar S (1999) DRONC, an ecdysone-inducible Drosophila caspase. Proc Natl Acad Sci U S A 96: 4307-4312
  14. Draizen TA, Ewer J and Robinow S (1999) Genetic and hormonal regulation of the death of peptidergic neurons in the Drosophila central nervous system. J Neurobiol 38: 455-465 https://doi.org/10.1002/(SICI)1097-4695(199903)38:4<455::AID-NEU2>3.0.CO;2-F
  15. Fujiwara H and Ogai S (2001) Ecdysteroid-induced programmed cell death and cell proliferation during pupal wing development of the silkworm, Bombyx mori. Dev Genes Evol 211: 118-123 https://doi.org/10.1007/s004270000131
  16. Garcia-Segura LM, Chowen JA, Parducz A and Naftolin F (1994) Gonadal hormones as promoters of structural synaptic plasticity: cellular mechanisms. Prog Neurobiol 44: 279-307 https://doi.org/10.1016/0301-0082(94)90042-6
  17. Hoffman KL and Weeks JC (2001) Role of caspases and mitochondria in the steroid-induced programmed cell death of a motoneuron during metamorphosis. Dev Biol 229: 517-536 https://doi.org/10.1006/dbio.2000.9987
  18. Holman SD, Collado P, Skepper JN and Rice A (1996) Postnatal development of a sexually dimorphic, hypothalamic nucleus in gerbils: a stereological study of neuronal number and apoptosis. J Comp Neurol 376: 315-325 https://doi.org/10.1002/(SICI)1096-9861(19961209)376:2<315::AID-CNE12>3.0.CO;2-K
  19. Hoskins SG and Grobstein P (1984) Induction of the ipsilateral retinothalamic projection in Xenopus laevis by thyroxine. Nature 307: 730-733 https://doi.org/10.1038/307730a0
  20. Jacobson MD, Weil M and Raff MC (1997) Programmed cell death in animal development. Cell 88: 347-354 https://doi.org/10.1016/S0092-8674(00)81873-5
  21. Jiang C, Baehrecke EH and Thummel CS (1997) Steroid regulated programmed cell death during Drosophila metamorphosis. Development 124: 4673-4683
  22. Kaellen B (1965) Degeneration and Regeneration in the Vertebrate Central Nervous System During Embryogenesis. Prog Brain Res 14: 77-96 https://doi.org/10.1016/S0079-6123(08)63740-1
  23. Kawata M (1995) Roles of steroid hormones and their receptors in structural organization in the nervous system. Neurosci Res 24: 1-46 https://doi.org/10.1016/0168-0102(96)81278-8
  24. Kerr JF, Wyllie AH and Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239-257 https://doi.org/10.1038/bjc.1972.33
  25. Kim MY, Lee BH, Kwon D, Kang H and Nassel DR (1998) Distribution of tachykinin-related neuropeptide in the developing central nervous system of the moth spodoptera litura. Cell Tissue Res 294: 351-365 https://doi.org/10.1007/s004410051185
  26. Kinch G, Hoffman KL, Rodrigues EM, Zee MC and Weeks JC (2003) Steroid-triggered programmed cell death of a motoneuron is autophagic and involves structural changes in mitochondria. J Comp Neurol 457: 384-403 https://doi.org/10.1002/cne.10563
  27. Kondo T, Yokokura T and Nagata S (1997) Activation of distinct caspase-like proteases by Fas and reaper in Drosophila cells. Proc Natl Acad Sci U S A 94: 11951-11956
  28. Lee BH, Kang H, Kwon D, Park CI, Kim WK and Kim MY (1998) Postembryonic development of leucokinin-like immunoreactive neurons in the moth Spodoptera litura. Tissue Cell 30: 74-85 https://doi.org/10.1016/S0040-8166(98)80008-6
  29. Lee CY, Cooksey BA and Baehrecke EH (2002) Steroid regulation of midgut cell death during Drosophila development. Dev Biol 250: 101-111 https://doi.org/10.1006/dbio.2002.0784
  30. Liu QA and Hengartner MO (1999) The molecular mechanism of programmed cell death in C. elegans. Ann N Y Acad Sci 887: 92-104 https://doi.org/10.1111/j.1749-6632.1999.tb07925.x
  31. Livingston DJ (1997) In vitro and in vivo studies of ICE inhibitors. J Cell Biochem 64: 19-26 https://doi.org/10.1002/(SICI)1097-4644(199701)64:1<19::AID-JCB4>3.0.CO;2-2
  32. Lockshin RA and Zakeri ZF (1990) Programmed cell death: new thoughts and relevance to aging. J Gerontol 45: B135-140
  33. Milligan CE, Prevette D, Yaginuma H, Homma S, Cardwell C, Fritz LC, Tomaselli KJ, Oppenheim RW and Schwartz LM (1995) Peptide inhibitors of the ICE protease family arrest programmed cell death of motoneurons in vivo and in vitro. Neuron 15: 385-393 https://doi.org/10.1016/0896-6273(95)90042-X
  34. Mills AC and Sengelaub DR (1993) Sexually dimorphic neuron number in lumbosacral dorsal root ganglia of the rat: development and steroid regulation. J Neurobiol 24: 1543- 1553 https://doi.org/10.1002/neu.480241108
  35. Mitchell HK (1978) An apparatus for microinjection of Drosophila. In The Genetics and Biology of Drosophila, 2a(ed. M. Ashburner & T. R. F. Wright, Academic Press, New York, pp. 150-155
  36. Mizoguchi A, Ohashi Y, Hosoda K, Ishibashi J and Kataoka H (2001) Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori: correlation with ecdysteroid secretion. Insect Biochem Mol Biol 31: 349-358 https://doi.org/10.1016/S0965-1748(00)00127-2
  37. Monsma SA and Booker R (1996) Genesis of the adult retina and outer optic lobes of the moth, Manduca sexta. I. patterns of proliferation and cell death. J Comp Neurol 367: 10-20 https://doi.org/10.1002/(SICI)1096-9861(19960325)367:1<10::AID-CNE2>3.0.CO;2-M
  38. Nordeen EJ, Nordeen KW, Sengelaub DR and Arnold AP (1985) Androgens prevent normally occurring cell death in a sexually dimorphic spinal nucleus. Science 229: 671-673 https://doi.org/10.1126/science.4023706
  39. Nordlander RH and Edwards JS (1969) Postembryonic brain development in the monarch butterfly, Danaus plexippus, L. I. Cellular events during brain morphogenesis. Wilhelm Roux Arch 162: 19-217 https://doi.org/10.1007/BF00581544
  40. Robinow S, Draizen TA and Truman JW (1997) Genes that induce apoptosis: transcriptional regulation in identified, doomed neurons of the Drosophila CNS. Dev Biol 190: 206- 213 https://doi.org/10.1006/dbio.1997.8696
  41. Schubiger M, Wade AA, Carney GE, Truman JW and Bender M (1998) Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis. Development 125: 2053-2062
  42. Streichert LC, Pierce JT, Nelson JA and Weeks JC (1997) Steroid hormones act directly to trigger segment-specific programmed cell death of identified motoneurons in vitro. Dev Biol 183: 95-107 https://doi.org/10.1006/dbio.1996.8501
  43. Susin SA, Zamzami N and Kroemer G (1998) Mitochondria as regulators of apoptosis: doubt no more. Biochim Biophys Acta 1366: 151-165 https://doi.org/10.1016/S0005-2728(98)00110-8
  44. Tata JR (1994) Hormonal regulation of programmed cell death during amphibian metamorphosis. Biochem Cell Biol 72: 581- 588 https://doi.org/10.1139/o94-077
  45. Terashima J, Yasuhara N, Iwami M, Sakurai S and Sakurai S (2000) Programmed cell death triggered by insect steroid hormone, 20-hydroxyecdysone, in the anterior silk gland of the silkworm, Bombyx mori. Dev Genes Evol 210: 545-558 https://doi.org/10.1007/s004270000100
  46. Thornberry NA and Lazebnik Y (1998) Caspases: enemies within. Science 281: 1312-1316 https://doi.org/10.1126/science.281.5381.1312
  47. Truman JW (1984) Cell death in invertebrate nervous systems. Annu Rev Neurosci 7: 171-188 https://doi.org/10.1146/annurev.ne.07.030184.001131
  48. Truman JW, Thorn RS and Robinow S (1992) Programmed neuronal death in insect development. J Neurobiol 23: 1295- 1311 https://doi.org/10.1002/neu.480230917
  49. Vaux DL and Korsmeyer SJ (1999) Cell death in development. Cell 96: 245-254 https://doi.org/10.1016/S0092-8674(00)80564-4
  50. Weeks JC, Roberts WM and Trimble DL (1992) Hormonal regulation and segmental specificity of motoneuron phenotype during metamorphosis of the tobacco hornworm, Manduca sexta. Dev Biol 149: 185-196 https://doi.org/10.1016/0012-1606(92)90271-H
  51. White K, Tahaoglu E and Steller H (1996) Cell killing by the Drosophila gene reaper. Science 271: 805-807 https://doi.org/10.1126/science.271.5250.805
  52. Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555-556 https://doi.org/10.1038/284555a0