Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Protein Composition and Biological Activities of Bombus ignitus Venom

호박벌 일벌독의 성분 분석 및 생리활성 탐색

  • Han, Sang-Mi (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Kwang-Gill (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Yeo, Joo-Hong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kweon, Hae-Yong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Woo, Soon-Ok (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Yoon, Hyung-Joo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Me-Ae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Won-Tae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Baek, Ha-Ju (Gyeongsang Buk-Do Government Public Institute of Health and Environmental)
  • 한상미 (농업과학기술원 농업생물부) ;
  • 이광길 (농업과학기술원 농업생물부) ;
  • 여주홍 (농업과학기술원 농업생물부) ;
  • 권해용 (농업과학기술원 농업생물부) ;
  • 우순옥 (농업과학기술원 농업생물부) ;
  • 윤형주 (농업과학기술원 농업생물부) ;
  • 김미애 (농업과학기술원 농업생물부) ;
  • 김원태 (농업과학기술원 농업생물부) ;
  • 백하주 (경상북도보건환경연구원)
  • Published : 2007.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1ng/ml to 100ng/ml. Expecially, the treatment of 100ng/ml B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256ug/ml, respectively.

호박벌 일벌독의 성분과 생리활성을 규명하기 위하여 단백질 성분분석과 암세포 생육 저해 효능, 항균력을 검토하였다. 이차원단백질 분석을 통해 호박별의 일벌독은 63개의 단백질이 존재하는 것으로 확인되었으며, 가장 많은 함량을 보이는 3개의 단백질을 염기서열 분석하였다. 그러나 이들 성분은 아직 밝혀지지 않은 성분으로 판단되었다. 호박벌 일벌독의 암세포 (간암; Hep3B, 폐암; A549, 유방암; BT-20, 위암; AGS) 에 대한 생육 저해능은 시료 농도가 증가함에 따라 증가하는 경향을 보이며 100ng/ml에서 간암세포 (Hep3B) 에 대한 생육 저해능이 55%로 가장 높았다. 항균활성에 E. faecium 과 S. sonnei에 대하여 최소발육억제농도와 최소살균농도 모두 각각 0.256ug/ml 로 강한 항균활성을 나타내었으며, 그 외의 피검균에 대해서도 비교적 높은 활성을 보였다. 이상의 결과로부터 호박벌 일벌독의 성분은 다른 벌의 독성분과는 차이를 보이며, 그 생리활성에 있어 약리학적 이용이 가능할 것으로 판단되었다.

Keywords

References

  1. Abbadie, C. and J.M. Besson. 1994. Chronic treatments with aspirin or acetaminophen reduce both the development of polyarthrithritis and Fos-like immunoreactivity in rat lumbar spinal cord. Pain. 57: 45-54 https://doi.org/10.1016/0304-3959(94)90106-6
  2. Argiolas, A. and J.J. Pisano. 1985. Bombolitins, a new class of mast cell degranulating peptides from the venom of the bumblebee Megabombus pennsylvanicus. J. Biol. Chem. 258: 13697-13702
  3. Dunn, J.D. and J.J. Killion. 1988. Effect of melittin on pituitaryadrenal responsiveness to stress. Acta. Endorinol. 119: 339-344
  4. Free, J.B. 1993. Insect pollination of crops. 2nd ed., pp. 684. Academic Press, London
  5. Gauldie, J., J.M. Hanson, R.A. Shipolini and C.A. Vernon. 1978. The structures of some peptides from bee venom. Eur. J. Biochem. 83: 405-410 https://doi.org/10.1111/j.1432-1033.1978.tb12106.x
  6. Habermann, E. and K.G. Reiz. 1965. On the biochemistry of bee venom peptides, melittin and apamin. Biochem. 343: 192-203
  7. Heinrich, B. 1979. Bumblebee economics. pp. 245. Harvard University Press. Cambridge, Massa
  8. Hwang, B.H., J.L. Zhao, K.P. Choi, S.W. Jung, E.J. Kim and S.S. Ham. 1996. The antimutagenic and anticancer effect of Taxus cuspidata extracts. J. Korean Soc. Food Sci. Nutr. 25: 1062-1068
  9. Kim, H.W, Y.B. Kwon, T.W. Ham, D.H. Rho, S.Y. Yoon, H.J. Lee, H.J Han, I.S. Yang, A.J. Beitz and J.H. Lee. 2003. Acupoint stimulation using bee venom attenuates formalininduced pain behavior and spinal cord for expression in rats. J. Vet. Med. Sci. 65: 349-355 https://doi.org/10.1292/jvms.65.349
  10. Lee, S.H. and L. Dumouhel. 1999. Taxanomic review of Genus Bombus (Hymenoptera, Apidae) from Korea. Ins. Koreana 16: 77-101
  11. Masahiro, M. 2000. Pollination of crops with bumblebee colonies in Japan. Honeybee Sci. 21: 17-25
  12. Moreau, S.J.M., A. Dingremont, G. Doury and P. Giordanengo. 2002. Effects of parasitism by Asobara tabida (Hymenoptera; Braconidae) on the develoment, survival and activity of Drosophila melanogaster larvae. J. Insect Physiol. 48: 337-347 https://doi.org/10.1016/S0022-1910(02)00051-3
  13. Murata, K., T. Shinada, Y. Ohfune, M.H. Hisada, A. Yasuda, H. Naoki and T. Nakajima. 2006. Novel biologicallly active peptides from the venom of Polistes rothneyi iwatai. Biol. Pharm. Bull. 29: 2493-2497 https://doi.org/10.1248/bpb.29.2493
  14. O'Connor, R., W. Rosenbrook and R. Erickson. 1964. Disc electrophoresis of Hymenoptera venoms and body proteins. Science. 145: 1320-1321 https://doi.org/10.1126/science.145.3638.1320
  15. Pattanaargson, S. and J. Roboz. 1996. Determination of hyaluronidase activity in venoms using capillary electrophoresis. Toxicon. 34: 1107-1117 https://doi.org/10.1016/0041-0101(96)00083-9
  16. Peiren, N., F. Vanrobaeys, D.C. de Graaf, B. Devreese., J.V. Beeumen and F.J. Jacobs. 2005. The protein composition of honeybee venom reconsidered by a proteomic approach. Biochem. Biophys. Acta 1752: 1-5 https://doi.org/10.1016/j.bbapap.2005.07.017
  17. Roll, A. and P. Shmid-Grendelmeier. 2005. Ultrarush immunotherapy in a patient with occupational allergy to bumblebee venom (Bombus terrestris). J. Invest. Allergol. Clin. Immunol. 15: 305-307
  18. Skehan, P., R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J.T. Warren, H. Bokesch, S. Kenney and M.R. Boyd. 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer Inst. 82: 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  19. Williams, P.H. 1998. An annotated checklist of bumble bees with an analysis of patterns of description (Hymenoptera: Apidae, Bombini). Bull. Nat. Hist. Mus. (Ent.) 6
  20. Winninghan K.M., C.D. Fitch, M. Schmidt and D.R. Hoffman. 2004. Hymenoptera venom protease allergens. J. Allergy Clin. Immunol. 114: 928-933 https://doi.org/10.1016/j.jaci.2004.07.043
  21. Yoon, H.J., S.E. Kim, K.Y. Lee, S.B. Lee and I.G. Park. 2005. Oviposition and colony development in the bumblebees, Bombus ignitus and B. terrestris depending on different pollen. Int. J. Indust. Entomol. 11: 99-105
  22. Yoon, H.J., S.E. Kim, Y.S. Kim and S.B. Lee. 2004. Colony development characteristics of the bumblebee queen, Bombus ignitus by the first oviposition day. Int. J. Indust. Entomol. 8: 139-143