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http://dx.doi.org/10.5656/KSAE.2007.46.2.229

Protein Composition and Biological Activities of Bombus ignitus Venom  

Han, Sang-Mi (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Lee, Kwang-Gill (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Yeo, Joo-Hong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kweon, Hae-Yong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Woo, Soon-Ok (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Yoon, Hyung-Joo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Me-Ae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Kim, Won-Tae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
Baek, Ha-Ju (Gyeongsang Buk-Do Government Public Institute of Health and Environmental)
Publication Information
Korean journal of applied entomology / v.46, no.2, 2007 , pp. 229-234 More about this Journal
Abstract
Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1ng/ml to 100ng/ml. Expecially, the treatment of 100ng/ml B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256ug/ml, respectively.
Keywords
Bombus ignitus; Bee venom; Biological activity; Antibacterial; Anticancer;
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