Journal of Microbiology

  • 제45권3호
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  • Pages.272-274
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  • 2007
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Multidrug-Resistant Providencia Isolates Carrying $bla_{PER-1},\;bla_{VIM-2}$, and armA

  • Lee, Hee-Woo (Department of Microbiology, Kyungpook National University, School of Medicine) ;
  • Kang, Hee-Young (Department of Microbiology, Kyungpook National University, School of Medicine) ;
  • Shin, Kyeong-Seob (Department of Laboratory Medicine, College of Medicine Chungbuk National University) ;
  • Kim, Jung-Min (Department of Microbiology, Kyungpook National University, School of Medicine)
  • 발행 : 2007.06.30
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초록

During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type $metallo-{\beta}-lactamase$ gene and PER-1 type extended spectrum ${\beta}-lactamase$ gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying $bla_{VIM-2},\;bla_{PER-1}$, and armA genes.

키워드

참고문헌

  1. Bahar, G., B. Erac, A. Mert, and Z. Gulay. 2004. PER-1 production in a urinary isolate of Providencia rettgeri. J. Chemother. 16, 343-346 https://doi.org/10.1179/joc.2004.16.4.343
  2. Docquier, J.D., F. Luzzaro, G. Amicosante, A. Toniolo, and G.M. Rossolini. 2001. Multidrug-resistant Pseudomonas aeruginosa producing PER-1 extended-spectrum serine-beta-lactamase and VIM-2 metallo-beta-lactamase. Emerg. Infect. Dis. 7, 910-911 https://doi.org/10.3201/eid0705.010528
  3. Galimand, M., P. Coruvalin, and T. Lambert. 2003. Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob. Agents Chemother. 47, 2565-2571 https://doi.org/10.1128/AAC.47.8.2565-2571.2003
  4. Gautom, R.K. 1997. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other Gram-negative organism in 1 day. J. Clin. Microbiol. 35, 2977-2980
  5. Jeong, S.H., K. Lee, Y. Chong, J.H. Yum, S.H. Lee, H.J. Choi, Y.M. Kim, K.H. Park, B.H. Han, S.W. Lee, and T.S. Jeong. 2003. Characterization of a new integron containing VIM-2, a metallo-${\beta}$-lactmase gene cassettes, in a clinical isolate of Enterobacter cloacae. J. Antimicrob. Chemother. 51, 397-400 https://doi.org/10.1093/jac/dkg047
  6. Lee, H., D. Yong, J.H. Yum, K.H. Roh, K. Lee, K. Yamane, Y. Arakawa, and Y. Chong. 2006. Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea. Diag. Microbiol. Infect. Dis. 56, 305-312 https://doi.org/10.1016/j.diagmicrobio.2006.05.002
  7. Lee, K., J.B. Lim, J.H. Yum, D. Yong, Y. Chong, J.M. Kim, and D.M. Livermore. 2002. $bla_{VIM-2}$ cassette-containing novel integrons in metallo-${\beta}$-lactamase-producing Pseudomonas aeruginosa and Pseudomonas putida isolates disseminated in a Korean hospital. Antimicrob. Agents Chemother. 46, 1053-1058 https://doi.org/10.1128/AAC.46.4.1053-1058.2002
  8. Lee, K., Y.S. Lim, D. Yong, J.H. Yum, and Y. Chong. 2003. Evaluation of the Hodge test and imipenem-EDTA double-disk synergy test for differentiating metallo-${\beta}$-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp. J. Clin. Microbiol. 41, 4623-4629 https://doi.org/10.1128/JCM.41.10.4623-4629.2003
  9. Mingeot-Leclercq, M-P., Y. Glupczynski, and P. Tulkens. 1999. Aminoglycosides: activity and resistance. Antimicrob. Agents Chemother. 43, 727-737
  10. National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, approved standard M7-A5, (6th ed). National Committee for Clinical Laboratory Standards, Wayne, Pa., USA
  11. Nordmann, P., E. Ronco, T. Naas, C. Duport, Y. Michel-Briand, and R. Labia. 1993. Characterization of a novel extendedspectrum ${\beta}$-lactamase from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 37, 962-969 https://doi.org/10.1128/AAC.37.5.962
  12. Peter, Z. 1992. Opportunistic Enterobacteriaceae, p. 551-552. In W.K. Joklik, H.P. Willett, D. Bernard Amos, and C.M. Wilfert (ed.). Zinsser Microbiology, (20th ed.) Appleton & Lange, Norwalk, Conneticut/San Maeto, CA, USA
  13. Poirel, L., L. Cavan, H. Vahaboglu, and P. Nordmann. 2005. Genetic environment and expression of the extended-spectrum ${\beta}$-lactamase $bla_{PER-1}$ gene in gram-negative bacteria. Antimicrob. Agents Chemother. 49, 1708-1713 https://doi.org/10.1128/AAC.49.5.1708-1713.2005
  14. Poirel, L., T. Lambert, S. Turkoglu, E. Ronco, J. Gaillard, and P. Nordmann. 2001. Characterization of class 1 integrons from Pseudomonas aeruginosa that contain the $bla_{VIM-2}$ carbapenem- hydrolyzing ${\beta}$-lactamase gene and of two novel aminoglycoside resistance gene cassettes. Antimicrob. Agents Chemother. 45, 546-552 https://doi.org/10.1128/AAC.45.2.546-552.2001
  15. Poirel, L., T. Naas, D. Nicolas, L. Collet, S. Bellais, J.D. Cavallo, and P. Nordmann. 2000. Characterization of VIM-2, a carbapenem- hydrolyzing metallo-${\beta}$-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France. Antimicrob. Agents Chemother. 44, 891-897 https://doi.org/10.1128/AAC.44.4.891-897.2000
  16. Shin, K.S., K. Han, J. Lee, S.B. Hong, B.R. Bon, S.J. Youn, J. Kim, and H.S. Shin. 2005. Imipenem-resistant Achromobacter xylosoxidans carrying blaVIM-2-containing class 1 integron. Diagn. Microb. Infect. Dis. 53, 215-220 https://doi.org/10.1016/j.diagmicrobio.2005.06.018
  17. Vahaboglü, H., S. Dodanli, C. Eroglu, R. Ozturk, G. Soyletir, I. Yildirim, and V. Avkan. 1996. Characterization of multipleantibiotic- resistant Salmonella typhimurium strains: molecular epidemiology of PER-1-producing isolates and evidence for nosocomial plasmid exchange by a clone. J. Clin. Microbiol. 34, 2942-2946
  18. Vahaboglu, H., R. Ozturk, G. Aygun, F. Coskunkan, F. Yaman, A. Kaygusuz, H. Leblebicioglu, I. Balik, K. Aydin, and M. Otkun. 1997. Widespread detection of PER-1-type extended-spectrum ${\beta}$-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: A nationwide multicenter study. Antimicrob. Agents Chemother. 41, 2265-2269
  19. Yan, J.J., J.J. Wu, W.C. Ko, S.H. Tsai, C.L. Chuang, H.M. Wu, Y.J. Lu, and J.D. Li. 2004. Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J. Antimicrob. Chemother. 54, 1007-1012 https://doi.org/10.1093/jac/dkh455
  20. Yong, D., J.H. Shin, S. Kim, Y. Lim, J.H. Yum, K. Lee, Y. Chong, and A. Bauernfeind. 2003. High Prevalence of PER-1 Extendedspectrum ${\beta}$-lactamase-producing Acinetobacter spp. in Korea. Antimicrob. Agents Chemother. 5, 1749-1751
  21. Yum, J.H., K. Yi, H. Lee, D. Yong, K. Lee, J.M. Kim, G.M. Rossolini, and Y. Chong. 2002a. Molecular characterization of metallo-${\beta}$-lactamase producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: identification of two new integron carrying the blaVIM-2 gene cassettes. J. Antimicrob. Chemother. 49, 837-840 https://doi.org/10.1093/jac/dkf043
  22. Yum, J.H., D. Yong, K. Lee, H.S. Kim, and Y. Chong. 2002b. A new integron carrying VIM-2 metallo-${\beta}$-lactamase gene cassette in a Serratia marcescens isolate. Diagn. Microbiol. Infect. Dis. 42, 217-219 https://doi.org/10.1016/S0732-8893(01)00352-2