Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Samul against Cisplatin in Primary Rat Organ of Corti Explant

시스플라틴 이독성에서 사물탕의 보호효과

  • Park, Chan-Ny (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University, School of Medicine) ;
  • Lee, Jeong-Han (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University, School of Medicine) ;
  • Lee, Sang-Heon (Otolaryngology-Head and Neck Surgery, Wonkwang University, School of Medicine)
  • 박찬희 (원광대학교 의과대학 미생물학교실) ;
  • 이정한 (원광대학교 의과대학 미생물학교실) ;
  • 이상헌 (원광대학교 이비인후-두경부외과학교실)
  • Published : 2007.02.25
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Abstract

The water extracts of Samultang (Samul) has been used for treatment of ischemic heart and brain damage in Oriental traditional medicine. However, little is known about the mechanism by which the water extract of Samul rescues cells from oxidative damages in cisplatin-induced ototoxicity. Cisplatin is a widely used chemotherapeutic agent that is also highly ototoxic. This study was designed to investigate the protective effects of Samul on ciplatin-induced ototoxicity in HEI-OC1 auditory cells and organ of Corti explant culture. Cisplatin markedly decreased the viability of HEI-OC1 auditory cells. However, treatment of HEI-OC1 cells with Samul significantly reduced cisplatin-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Cisplatin induced cytotoxicity in isolated and cultured hair cell progenitors from postnatal rat cochleae. These progenitor cells are isolated from the lesser epithelial ridge (LER, or outer spiral sulcus cell) area of pre-plated neonatal rat cochlear segments. However, Samul completely protected the morphological changes of organ of Corti and LER. Taken together, these data suggest that the protective effects of the water extracts of Samul against cisplatin may be mediated by the reduction of intracellular peroxide generation.

Keywords

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