Browse > Article

Protective Effect of Samul against Cisplatin in Primary Rat Organ of Corti Explant  

Park, Chan-Ny (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University, School of Medicine)
Lee, Jeong-Han (Vestibulocochlear Research Center & Department of Microbiology, Wonkwang University, School of Medicine)
Lee, Sang-Heon (Otolaryngology-Head and Neck Surgery, Wonkwang University, School of Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.1, 2007 , pp. 214-218 More about this Journal
Abstract
The water extracts of Samultang (Samul) has been used for treatment of ischemic heart and brain damage in Oriental traditional medicine. However, little is known about the mechanism by which the water extract of Samul rescues cells from oxidative damages in cisplatin-induced ototoxicity. Cisplatin is a widely used chemotherapeutic agent that is also highly ototoxic. This study was designed to investigate the protective effects of Samul on ciplatin-induced ototoxicity in HEI-OC1 auditory cells and organ of Corti explant culture. Cisplatin markedly decreased the viability of HEI-OC1 auditory cells. However, treatment of HEI-OC1 cells with Samul significantly reduced cisplatin-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Cisplatin induced cytotoxicity in isolated and cultured hair cell progenitors from postnatal rat cochleae. These progenitor cells are isolated from the lesser epithelial ridge (LER, or outer spiral sulcus cell) area of pre-plated neonatal rat cochlear segments. However, Samul completely protected the morphological changes of organ of Corti and LER. Taken together, these data suggest that the protective effects of the water extracts of Samul against cisplatin may be mediated by the reduction of intracellular peroxide generation.
Keywords
Cisplatin; ototoxicity; Samul; Protective effect; Organ of Corti;
Citations & Related Records
연도 인용수 순위
  • Reference
1 McAlpine, D., Johnstone, B.M. The ototoxic mechanism of cisplatin. Hear Res. 47:191-203, 1990   DOI   ScienceOn
2 신민교. 최신한방임상진료, 영림사, pp 389-390, 1988.
3 南昌圭. 四物湯이 血管內皮細胞에 미치는 영향. 원광대학교 한의과대학 대학원. 1997
4 이근후. 최신임상정신의학. 서울, 하나의학사, pp 138, 216-228, 1988
5 Humes, H.D. Insights into ototoxicity. Analogies to nephrotoxicity, Ann. NY. Acad. Sci. 884:15-18, 1999   DOI
6 Park, C., So, H.S., Shin, S.H., Choi, J.Y., Lee, I., Kim, J.K., Chung, S.Y., Park, R. The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide through prevention of mitochondrial dysfunction and activation of caspases pathway. Phytother Res. 21:81-88, 2007   DOI   ScienceOn
7 신영경. 四物湯의 季節別 倍味,加味에 依한 생쥐의 體重變化 및 免疫機能에 미치는 影響, 대구한의과대 대학원. 1988
8 洪茂昌 外. 四物湯 투여가 家犬의 赤血球像에 미치는 영향에 관한 연구, 경희한의대논문집, 1:117-120, 1978
9 Fram, R.J. Cisplatin and platinum analogues: Recent advances. Curr Opin Oncol. 4:1073-1079, 1992   DOI   ScienceOn
10 Park, C., So, H.S., Kim, S.J., Youn, M.J., Moon, B.S., Shin, S.H., Lee, I., Moon, S.K., Park, R. Samul extract protects against the $H_{2}O_{2}$-induced apoptosis of H9c2 cardiomyoblasts via activation of extracellular regulated kinases (Erk) 1/2. Am J Chin Med. 34:695-706, 2006   DOI   ScienceOn
11 So, H.S., Park, C., Kim, H.J., Lee, J.H., Park, S.Y., Lee, J.H., Lee, Z.W., Kim, H.M., Kalinec, F., Lim, D.J., Park, R. Protective effect of T-type calcium channel blocker flunarizine on cisplatin-induced death of auditory cells. Hear Res. 204:127-139, 2005   DOI   ScienceOn
12 姜昌洙. 四物湯 전탕액이 가토의 혈압강하에 미치는 영향, 원광대학교 대학원. 1984
13 신민교. 임상본초학, 영림사, pp 219-223, 249-250, 1998
14 朴鍾雲 外. 四物湯이 老化白鼠 뇌조직의 생화학적 변화에 미치는 영향. 대한한방내과학회지 19(1):185-201, 1998
15 Rybak, L.P., Whitworth, C., Somani, S. Application of antioxidants and other agents to prevent cisplatin ototoxicity. Laryngoscope. 109:1740-17444, 1999   DOI   ScienceOn
16 楊維傑 編. 黃帝內經靈樞譯解, 台聯, 國風出版社, p 199, 200, 271, 488, 1976
17 柳道坤 編著. 東醫生理學講議, 익산, 圓光大學校 出版局, pp 255-263. 1999
18 金完熙, 崔達永 共編. 臟腑辨證論治, 서울, 成輔社, p 57, 58, 1993
19 Yu, H.H., Kim, Y.H., Jung, S.Y., Shin, M.K., Park, R.K., So, H.S., Kim, K.Y., Lee, D.H., You, Y.O. Rehmannia glutinosa Activates Intracellular Antioxidant Enzyme Systems in Mouse Auditory Cells. Am J Chin Med. 34:1083-1093, 2006   DOI   ScienceOn
20 陳師文. 太平惠民和劑局方, 臺北, 旋風出版社, p 242, 1975
21 權在龍. 四物湯 및 계절별 활용방이 혈액에 미치는 영향, 대구한의과대학 대학원. 1991
22 康舜洙. 바른方劑學, 대성문화사, pp 122-123, 1996
23 河智容. 四物湯 및 四君子湯이 endotoxin으로 유발된 혈전증에 미치는 영향, 경희대학교 대학원. 1998
24 Zhai, S., Shi, L., Wang, B.E., Zheng, G., Song, W., Hu, Y., Gao, W.Q. Isolation and Culture of Hair Cell Progenitors from Postnatal Rat Cochleae, J Neurobiol. 65(3):282-293, 2005   DOI   ScienceOn
25 尹吉榮. 동의임상방제학, 명보출판사, pp 254-263, 1992