Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Composition Comprising the Extract of Salicis Radicis Cortex for Immune Activity

유근피 추출물을 함유하는 면역증강용 조성물

  • Park, Gil-Soon (Department of Infection Biology, Zoonosis Research Center Wonkwang University, College of Medicine, Department of Oriental Medical Prescription, College of Oriental Medicine Wonkwang University) ;
  • Chang, In-Ae (Department of Infection Biology, Zoonosis Research Center Wonkwang University, College of Medicine, Department of Oriental Medical Prescription, College of Oriental Medicine Wonkwang University) ;
  • Kim, Youn-Chul (College of Pharmacy Wonkwang University) ;
  • Lee, Moo-Hyung (Department of Infection Biology, Zoonosis Research Center Wonkwang University, College of Medicine, Department of Oriental Medical Prescription, College of Oriental Medicine Wonkwang University) ;
  • Shin, Hye-Young (Department of Infection Biology, Zoonosis Research Center, College of Medicine, Wonkwang University) ;
  • Choi, Du-Young (Department of Pediatrics, College of Medicine, Wonkwang University) ;
  • Park, Hyun (Department of Infection Biology, Zoonosis Research Center, College of Medicine, Wonkwang University) ;
  • Yun, Yong-Gab (Department of Infection Biology, Zoonosis Research Center Wonkwang University, College of Medicine, Department of Oriental Medical Prescription, College of Oriental Medicine Wonkwang University)
  • 박길순 (원광대학교 인수공통감염병연구센터 및 의과대학 감염생물학교실, 원광대학교 한의과대학 방제학교실) ;
  • 장인애 (원광대학교 인수공통감염병연구센터 및 의과대학 감염생물학교실, 원광대학교 한의과대학 방제학교실) ;
  • 김윤철 (원광대학교 약학대학) ;
  • 이무형 (원광대학교 인수공통감염병연구센터 및 의과대학 감염생물학교실, 원광대학교 한의과대학 방제학교실) ;
  • 신혜영 (원광대학교 인수공통감염병연구센터 및 의과대학 감염생물학교실) ;
  • 최두영 (원광대학교 의과대학 소아과학교실) ;
  • 박현 (원광대학교 인수공통감염병연구센터 및 의과대학 감염생물학교실) ;
  • 윤용갑 (원광대학교 인수공통감염병연구센터 및 의과대학 감염생물학교실, 원광대학교 한의과대학 방제학교실)
  • Published : 2007.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

In the recent, increased concern has been focused on the pharmacology and clinical utility of herbal extracts and derivatives as a drug or adjunct to chemotherapy and immunotherapy. Salicis Radicis Cortex, A decoction has been mainly used for improvement of ozena and a diuretic effect in oriental medicine, but there was no study on the molecular mechanism of Salicis Radicis Cortex as an immunomodulator. Here we investigated the role of the aqueous extract of Salicis Radicis Cortex in the expression of inflammatory mediators, surface molecule, and related receptors in vitro and in vivo. In murine macrophage RAW 264.7 cells and peritoneal macrophages of C57BL/6N mice, water extract of Salicis Radicis Cortex increased the production of secretary TNF-alpha and Nitric oxide, and the expression level of CD14, LPS co-receptor and CD86, co-stimulatory molecule compared to negative natural extract ex vivo. Moreover, i.p. injection of water extract of Salicis Radicis Cortex significantly increased the secretion level of IFN-gamma and TNF-alpha, IL-2, IL-4 and IL-5 in serum of mice in vivo. Taken together, these results suggest that Salicis Radicis Cortex may regulate the immune response by secreting Th1 and Th2 types of cytokines in vivo and the possibility of its as natural immunostimulator.

Keywords

References

  1. Han, S.B., Lee, C.W., Kang, M.R., Yoon, Y.D., Kang, J.S., Lee, K.H., Yoon, W.K., Lee, K.H., Park, S.K., Kim, H.M. Pectic polysaccharide isolated from Angelica gigas Nakai inhibits melanoma cell metastasis and growth by directly preventing cell adhesion and activating host immune functions. cancer letters, 1-10, 2006
  2. Yang, H.Z., Xu, S., Liao, X.Y., Zhang, S.D., Liang, Z.L., Liu, B.H., Bai, J.Y., Jiang, C., Ding, J., Cheng, G.F., Liu, G. A Novel Immunostimulator, N2-[-O-Benzyl-N-(acetylmuramyl)- L-alanyl-D-isoglutaminyl]-N6-trans-(m-nitrocinnamoyl)-L-lysine, and Its Adjuvancy on the Hepatitis B Surface Antigen. J. Med. Chem. 48:5112-5122, 2005 https://doi.org/10.1021/jm0493313
  3. Lin, Y.L., Liang, Y.C., Lee, S.S., Chiang, B.L. Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-${\kappa}$ B and p38 mitogen-activated protein kinase pathways. Journal of Leukocyte Biology. 78:533-543, 2005 https://doi.org/10.1189/jlb.0804481
  4. Hsu, H.Y., Hua, K.G., Lin, C.C., Lin, C.H., Hsu, J., Wong, C.H. Extract of Reishi Polysaccharides Induces Cytokine Expression via TLR4-Modulated Protein Kinase Signaling Pathways. The Journal of Immunology. 173:5989-5999, 2004 https://doi.org/10.4049/jimmunol.173.10.5989
  5. Shao, B.M., Dai, H., Xu, W., Lin, Z.B., Gao, X.M. Immune receptors for polysaccharides from Ganoderma lucidum. Biochemical and Biophysical Research Communications. 323:133-141, 2004 https://doi.org/10.1016/j.bbrc.2004.08.069
  6. Chen, H.S., Tsai, Y.F., Lin, S., Lin, C.C., Khoo, K.H., Lin, C.H., Wong, C.H. Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides. Bioorganic & Medicinal Chemistry. 12:5595-5601, 2004 https://doi.org/10.1016/j.bmc.2004.08.003
  7. Han, S.B., Park, S.K., Ahn, H.J., Yoon, Y.D., Kim, Y.H., Lee, J.J., Lee, K.H., Moon, J.S., Kim, H.C., Kim, H.C., Kim, H.M. Characterization of B cell membrane receptorsof polysaccharide isolated from the root of Acanthopanax koreanum. International Immunopharmacology. 3:683-691, 2003 https://doi.org/10.1016/S1567-5769(03)00056-0
  8. Fu, S.L., Hsu, Y.H., Lee, P.Y., Hou, W.C., Hung, L.C., Lin, C.H., Chen, C.M., Huang, Y.J. Dioscorin isolated from Dioscorea alata activates TLR4-signaling pathways and induces cytokine expression in macrophages. Biochemical and Biophysical Research Communications. 339:137-144, 2006 https://doi.org/10.1016/j.bbrc.2005.11.005
  9. Nakaya, T.A. Panax ginseng Induces Production of Proinflammatory Cytokines via Toll-like Receptor. Journal of Interferon & Cytokine Research. 24:93-100, 2004 https://doi.org/10.1089/107999004322813336
  10. Han, S.B., Yoon, Y.D., Ahn, H.J., Lee, H.S., Lee, C.W., Yoon, W.K., Park, S.K., Kim, H.M. Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. International Immunopharmacology. 3:1301-1312, 2003 https://doi.org/10.1016/S1567-5769(03)00118-8
  11. Wang, M., Guilbert, L.J., Ling, L., Li, J., Wu, Y.Q., Xu, S., Pang, P., Shan, J.J. Immunomodulating activity of CVT-E002, a proprietary extract from North American ginseng (panax quinquefolium), Journal of Pharmacology. 53:1515-1523, 2001 https://doi.org/10.1211/0022357011777882
  12. Wang, M., Guillbert, L.J., Li, J., Wu, Y.Q., Pang, P., Basu, T.K., Shan, J.J. A proprietary extract from North American ginseng(panax quinquefolium) enhances IL-2 and IFN- productions in murine spleen cells induced by Con-A. International Immunopharmacology. 4:311-315, 2004 https://doi.org/10.1016/j.intimp.2003.12.002
  13. Roswitha F., Thomas, M., Brigitte, T., Paul, G.S. Stimulation of nitric oxide synthesis by the aqueous extract of Panax ginseng root in RAW 264.7 cells. British Journal of Pharmacology. 134:1663-1670, 2001 https://doi.org/10.1038/sj.bjp.0704425
  14. Pae, H.O., Oh, H., Yun, Y.G., Oh, G.S., Jang, S.I., Hwang, K.M., Kwon, T.O., Lee, H.S., Chung, H.T. Imperatorin, a furanocoumarin from Angelica dahurica(Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 Cells. Pharmacol Toxicol. 91(1):40-48, 2002 https://doi.org/10.1034/j.1600-0773.2002.910107.x
  15. Schinella, G.R., Tournier, H.A., Prieto, J.M., Rios, J.L., Buschiazzo, H., Zaidenberg, A. Inhibition of Trypanosoma cruzi growth by medical plant extracts, Fitoterapia. 73(7-8):569-575, 2002 https://doi.org/10.1016/S0367-326X(02)00246-0
  16. Shin J.Y., Song, J.Y., Yun, Y.S., Yang, H.O., Rhee, D.K., Pyo, S. Immunostimulating effets of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol Immunotoxicol. 24(3):469-482, 2002 https://doi.org/10.1081/IPH-120014730
  17. Wang, J.Z., Mao, X.J., Ito, H., Shimura, K. Immunomodulatory activity of polysaccharide from Acanthopanax obovatus roots. Planta Med. 57(4):335-336, 1991 https://doi.org/10.1055/s-2006-960111
  18. Park, H.H., Seo, Y.B. 桑寄生의 免疫調節作用에 對한 實驗的 硏究. 大韓本草學會誌. 16(1), 2001