Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Study on the Effect of Gwanjulbang-5 in Rheumatoid Arthritis

관절 5호방의 류마토이드 관절염 치료효과에 대한 실험적 연구

  • Choi, Jae-Young (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Daejeon University) ;
  • Heo, Dong-Seok (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Daejeon University) ;
  • Yoon, Il-Ji (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Daejeon University) ;
  • Oh, Min-Seok (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Daejeon University)
  • 최재영 (대전대학교 한의과대학 한방재활의학과) ;
  • 허동석 (대전대학교 한의과대학 한방재활의학과) ;
  • 윤일지 (대전대학교 한의과대학 한방재활의학과) ;
  • 오민석 (대전대학교 한의과대학 한방재활의학과)
  • Published : 2007.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to know the effect of Gwanjulbang-5(hereinafter refer to GJB-5) to on Rheumatoid Arthritis by using human fibroblast-like synoviocytes(hFLS). We performed several experimetal items : that is cytotoxicity of GJB-5, mRNA expression of pro-imflammatory cytokines in hFLS and production of NO, ROS. The results were obtained as follows : GJB-5 reduced the production of pro-inflammatory cytokines TNF-${\alpha}$, IL-1${\beta}$, IL-6, IL-8 in hFLS, increased the production of TIMP-1. As well as GJB-5 reduced the production of ICAM-1, MMP-3, NOS-II, the production of NO and ROS, and the proliferation of hFLS in proportion to the concentration of GJB-5. In conclusion, these results shows that GJB-5 had immunomodulatory effects in treating rheumatoid arthritis.

Keywords

References

  1. 양형인. 류마티스 관절염의 최신 치료. 경희의학, 20(1):1-3, 2004
  2. 허 준. 동의보감. 서울, 법인문화사, p 979, 1999
  3. 최도영 외 10인. 류마티스 관절염에 대한 한약의 면역학적 연구동향. 대한침구학회지 21(4):177-196, 2004
  4. 대한정형외과학회. 정형외과학. 서울, 최신의학사, p 175, 2004
  5. 김진호, 한태륜. 재활의학. 서울, 군자출판사, p 503, 2004
  6. 김영태, 이재동, 이윤호. 최근 RA와 관련된 임상 및 실험 논문의 경향. 대한침구학회지 19(5):190-194, 2002
  7. 이중휘. Type II Collagen으로 誘發된 關節炎에 대한 疏經活血湯加味의 免疫 抑制 效果. 대전대학교대학원, 2004
  8. 諸正鎭. 血府逐瘀湯이 Adjuvant誘發 關節炎에 미치는 影響. 경희대학교대학원, 1993
  9. 申敏湜. 防風湯이 Adjuvant誘發 關節炎에 미치는 影響. 경희대학교대학원, 1994
  10. 金荀中. Adjuvant관절염에 疎經活血湯合黃連解毒湯이 미치는 實驗的 硏究. 대전대학교대학원, 1992
  11. 李世鉉. 行濕流氣散이 Adjuvant關節炎에 미치는 影響. 대전대학교대학원, 1992
  12. 곽중문. 疎風活血湯加味가 류마토이드 關節炎 患者에서 分離한 synoviocytes에 대한 免疫反應과 collagen으로 誘發된 생쥐의 關節炎 抑制에 관한 硏究. 대전대학교대학원, 2002
  13. 申炳熙. 桂枝芍藥知母湯이 第II型 Collagen誘發 關節炎의 抗體에 미치는 影響. 경희대학교대학원, 1994
  14. 李昊根. 大羌活湯이 제II형 Collagen誘發 關節炎의 抗體에 미치는 影響. 경희대학교대학원, 1994
  15. 정수현. 三痺湯이 Collagen으로 유발된 관절염에 미치는 영향. 한방재활의학과학회지 10(1):173-183, 2000
  16. Firestein, G.S., Echeverri, F., Yeo, M., Zvaifler, N.J., Green, D.R. Somatic Mutations in the p53 Tumor Suppressor Gene in Rheumatoid Arthritis Synovium. Proc Natl Acad Sci USA. 94(20):10895-10900, 1997
  17. Reme, T., Travaglio, A., Gueydon, E., Adla, L., Jorgensen, C., Sany, J. Mutations of the p53 Tumor Supressor Gene in Erosive Rheumatoid Synovial Tissue. Clin Exp Immunol. 111(2):353-358, 1998 https://doi.org/10.1046/j.1365-2249.1998.00508.x
  18. 이상훈 외 7인. 무작위 대조 이중맹검 시험을 통한 봉독 약침의 류마티스 관절염 치료 효과 연구. 대한침구학회지 20(6):80-88, 2003
  19. 張仲景. 金?要略方論. 臺北, 대련국풍출판사, pp 32-33, 1973
  20. 朱震亨. 丹溪心法附餘. 서울, 대성문화사, p 206, 1982
  21. 王 燾. 外臺秘要. 北京, 華河出版社, p 266, 1993
  22. 강인수. 비증치료의 용약에 관한 소고. 대한한의학회지 11(1):245-252, 1999
  23. 楊維傑 編著. 黃帝內經素問譯解. 台北市, 台聯國風出版社, pp 95-96, 328-336, 1984
  24. 陳無擇. 三因方(卷3). 大連,. 國風出版社, pp 2-3, 1977
  25. 黃文東 外. 實用中醫內科學. 上海, 上海科學技術出版社, pp 554-569, 1986
  26. 權宰植 外. 痺證. 서울, 鼎談, pp 207-208, 214-215, 219-222, 270, 1993
  27. Choy, E.H., Panayi, G.S. Cytokine pathways and joint inflammation in rheumatoid arthritis. N Engl J Med. 344: 907-916, 2001 https://doi.org/10.1056/NEJM200103223441207
  28. Ribbens, C., Andre, B., Kaye, O., Kaiser, M.J., Bonnet, V., de Groote, D., Franchimont, N., Malaise, M.G. Increased synovial fluid levels of interleukin-12, sCD25 and sTNF-RII/sTNF-RI ratio delineate a cytokine pattern characteristic of immune arthropathies. Eur Cytokine Newt. 11: 669-676, 2000
  29. Schlaak, J.F., Pfers, I., Meyer Zum Buschenfelde, K.H., Marker-Hermann, E. Differant cytokine profiles in the synovial fluid of patient with osteoarthritis, rheumatoid arthritis and seronegative spondyloarthropathies. Clin Exp Rheumarol. 14: 63-88, 2002
  30. Punzi, L., Calo, L., Plebani, M. Clinical significance of cytokine determination in synovial fluid. Crit Rev Clin Lab Sci. 39: 63-88, 2002 https://doi.org/10.1080/10408360290795448
  31. Arend, W.P. Physiology of cytokine pathways in rheumatoid arthritis. Arthritis Rheum. 45: 101-106, 2001 https://doi.org/10.1002/1529-0131(200102)45:1<101::AID-ANR90>3.0.CO;2-7
  32. Wingard, J.R., Demetri, G.D. Clinical applications of cytokines and growth factors. Norwell. Kluwer Academic publishers. p 39, 1999
  33. Kutukculer, N., Caglayan, S., Aydogdu, F. Study of pro-inflammatory(TNF-$\alpha$, IL-1$\alpha$, IL-6) and T-cell-derived (IL-2, IL-4) cytokines in plasma and synovial fluid of patient with juvenile chronic arthritis : correlation with clincal and laboratory parameters. Clin Rheumatol. 17: 288-292, 1998 https://doi.org/10.1007/BF01451007
  34. Hirai, Y., Honda, S., Ukei, Y., Yamasaki, S., Urayama, S., Kamachi, M., Kawakami, A., Ida, H., Kita, M. Fukuda, T., Shibatomi, K., Kawabe, Y., Aoyagi, T., Eguchi, K. Effect of nitric oxide in matric metalloproteinase-2 production by rheumatoid synovial cells. Life Sci. 68: 913-920, 2001 https://doi.org/10.1016/S0024-3205(00)00998-X
  35. Rausch-Fan, C., Matejka, M. From plaque formation to periodintal disease is there a role for nitric oxide. Eur J Clin Invest. 31: 833-835, 2001 https://doi.org/10.1046/j.1365-2362.2001.00903.x
  36. Kendall, H.K., Marshell, R.I., Bartold, P.M. Nitric oxide and tissue destruction. Oral Dis. 7: 2-10, 2001 https://doi.org/10.1034/j.1601-0825.2001.70102.x
  37. Halliwell, B. Oral inflammation and reactive species : a missed opportunity. Oral Dis. 6: 136-137, 2000 https://doi.org/10.1111/j.1601-0825.2000.tb00324.x
  38. Waddington, R.J., Moseley, R., Embery, G. Reactive oxygen species : a potential role in the pathogenesis of periodontal disease. Oral Dis. 6: 138-151, 2000 https://doi.org/10.1111/j.1601-0825.2000.tb00325.x
  39. 이상인 외 5인. 한약임상응용. 서울, 성보사, pp 38, 152, 188, 208, 225, 267, 271, 275, 293-294, 316-317, 321, 324, 342, 345-346, 354, 358, 360, 1998
  40. Ribbens, C., Andre, B., Kaye, 0., Kaiser, M.J., Bonnet, V., de Groote, D., Franchimont, N., Malaise, M.G. Increased synovial fluid levels of interleukin-12, sCD25 and sTNF-RII/sTNF-RI ratio delineate a cytokine pattern characteristic of immune arthropathies. Eur Cytokine Newt. 11: 669-676, 2000
  41. Schlaak, J.F., Pfers, I., Meyer Zum Buschenfelde, K.H., Marker-Hermann, E. Differant cytokine profiles in the synovial fluid of patient with osteoarthritis, rheumatoid arthritis and seronegative spondyloarthropathies. Clin Exp Rheumarol. 14: 63-88, 2002