Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The effect of irradiated cancellous human bone on exophytic bone formation in residual ridge of the beagle dog

방사선 조사 망상골이 외방형 골형성에 미치는 영향

  • Chung, Mi-Hyun (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Herr, Yeek (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Kwon, Young-Hyuk (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Park, Joon-Bong (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Chung, Jong-Hyuk (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University)
  • 정미현 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 허익 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 권영혁 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 박준봉 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 정종혁 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소)
  • Published : 2007.12.31
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Abstract

The purpose of this study was to evaluate exophytically vertical bone formation in residual ridge of the beagle dog by the concept of guided bone regeneration with a titanium reinforced e-PTFE membrane combined with irradiated cancellous human bone. Twelve male beagle dogs(mean age 1.5 years and mean weight 12kg) were used for this study. The alveolar ridges after extraction of all mandibular premolars were surgically and horizontally removed. At 8 weeks after extractions, full-thickness flap was reflected and cortical bone was removed with round bur and copious irrigation. Rectangular parallelepiped(10mm in length, 5mm in width, and 4mm in height) bended with titanium-reinforced e-PTFE(TR e-PTFE) membrane was placed on the decorticated alveolar ridge, fixed with metal pins and covered with full-thickness flap and assigned as a control group. Test groups ere treated with TR e-PTFE membrane filled with irradiated cancellous human bone. Of twelve beagle dogs, four control dogs and four test dogs without membrane exposure to oral cavity were sacrificed at 8 and 16 weeks respectively. The surgical sites were dissected out, fixed in 4% buffered formaldehyde, dyed using a Villanueva staining technique, and processed for embedding in plastic resin. The cutting and grinding methods were routinely processed for histologic and histomophometric analyis of exophytic bone formation as well as statistical analysis. The results of this study were as follows: 1. Exophytic bone formation in the both of experimental groups was increased respectively after surgery from 23.40% at 8 weeks to 46.26% at 16 weeks in the control groups, from 40.23% at 8 weeks to 47.11% at 16 weeks in the test groups(p<0.05). 2. At 8 weeks after surgery, exophytic bone formation was made 40.23% in the test groups and 33.40% in the control groups. Exophytic bone formation was significantly made in the test group more than in the control group. At 16 weeks after surgery, exophytic bone formation was made 44.11% in the test groups and 46.26% in the control groups. Exophytic bone formation was made in the test groups more than in the control groups, but there was no statistically significant differences. 3. The membrane was fixed with metal pins to closely contact it to the bone surface. So, collapse and deviation of the membrane could be prevented and in growth of connective tissue also could be blocked from the periphery of the membrane. On the basis of these findings, wee suggest that intraoral experimental model for exophytic bone formation may be effective to evaluate the effect of bone graft material. And it indicates that combined use of membrane and ICB graft material is more effective than use of membrane only for exophytic bone formation.

Keywords

References

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