Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The Analysis of Bone regenerative effect with carriers of bone morphogenetic protein in rat calvarial defects

백서두개골 결손부에서 BMP전달체의 골재생효과분석

  • Jung, Sung-Won (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Jung, Jee-Hee (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Chae, Gyung-Joon (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Jung, Ui-Won (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Chang-Sung (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Chai, Jung-Kiu (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Chong-Kwan (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Choi, Seong-Ho (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University)
  • 정성원 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 정지희 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 채경준 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 정의원 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 김창성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 조규성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 채중규 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 김종관 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 최성호 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소)
  • Published : 2007.12.31
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Abstract

Bone morphogenetic proteins have been shown to possess significant osteoinSductive potential, but in order to take advantage of this effect for tissue engineering, carrier systems are essential. Successful carrier systems must enable vascular and cellular invasion, allowing BMP to act as a differentiation factor. The carrier should be reproducible, non-immunogenic, moldable, and space-providing, to define the contours of the resulting bone. The purpose of this study was to review available literature, in comparing various carriers of BMP on rat calvarial defect model. The following conclusions were deduced. 1. Bone regeneration of ACS/BMP, ${\beta}-TCP/BMP$, FFSS/BMP, $FFSS/{\beta}-TCP/BMP$, MBCP/BMP group were significantly greater than the control groups. 2. Bone density in the ACS/BMP group was greater than that in ${\beta}-TCP$, FFSS, $FFSS/{\beta}-TCP$ carrier group. 3. Bone regeneration in FFSS/BMP group was less than in ACS/BMP, ${\beta}-TCP/BMP$, MBCP/BMP group. However, New bone area of $FFSS/{\beta}-TCP/BMP$ carrier group were more greater than that of FFSS/BMP group. ACS, ${\beta}-TCP$, FFSS, $FFSS/{\beta}-TCP$, MBCP were used for carrier of BMP. However, an ideal carrier which was reproducible, non-immunogenic, moldable, and space-providing did not exist. Therefore, further investigation are required in developing a new carrier system.

Keywords

References

  1. Lindholm TC, Lindholm TS, Marttinen A, Urist MR. Bovine bone morphogenetic protein (bBMP/NCP)-induced repair of skull trephine defects in pigs. Clin Orthop Relat Res 1994(301) :263-270
  2. Urist MR, Strates BS. Bone morphogenetic protein. J Dent Res 1971;50(6):1392-1406 https://doi.org/10.1177/00220345710500060601
  3. Takaoka K, Ono K, Amitani K et al. Solubilization and concentration of a bone-inducing substance from a murine osteosarcoma. Clin Orthop Relat Res 1980 (148):274-280
  4. Wozney JM. The bone morphogenetic protein family and osteogenesis. Mol Reprod Dev 1992;32(2):160-167 https://doi.org/10.1002/mrd.1080320212
  5. Rosen V, Thies RS. The BMP proteins in bone formation and repair. Trends Genet 1992;8(3):97-102 https://doi.org/10.1016/0168-9525(92)90197-C
  6. Reddi AH, Cunningham NS. Bone induction by osteogenin and bone morphogenetic proteins. Biomaterials 1990;11:33-34
  7. Hong SJ, Kim CS, Han DK et al. The effect of a fibrin-fibronectin/beta-tricalciurn phosphate/recombinant human bone morphogenetic protein-2 system on bone formation in rat calvarial defects. Biomaterials 2006;27(20):3810-3816 https://doi.org/10.1016/j.biomaterials.2006.02.045
  8. King GN, Cochran DL. Factors that modulate the effects of bone morphogenetic protein-induced periodontal regeneration: a critical review. J Periodontol 2002;73(8):925-936 https://doi.org/10.1902/jop.2002.73.8.925
  9. Wikesjo UM, Guglielmoni P, Promsudthi A et al. Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment. J Clin Periodontol 1999;26(6):392-400 https://doi.org/10.1034/j.1600-051X.1999.260610.x
  10. Kenley R, Marden L, Turek T et al. Osseous regeneration in the rat calvarium using novel delivery systems for recombinant human bone morphogenetic protein-2 (rhBMP-2). J Biomed Mater Res 1994;28(10): 1139-1147 https://doi.org/10.1002/jbm.820281004
  11. Gao TJ, Lindholm TS, Kommonen B et al. Enhanced healing of segmental tibial defects in sheep by a composite bone substitute composed of tricalcium phosphate cylinder, bone morphogenetic protein, and type IV collagen. J Biomed Mater Res 1996;32(4):505-512 https://doi.org/10.1002/(SICI)1097-4636(199612)32:4<505::AID-JBM2>3.0.CO;2-V
  12. Laffargue P, Hildebrand HF, Rtaimate M et al. Evaluation of human recombinant bone morphogenetic protein-2-loaded tricalcium phosphate implants in rabbits' bone defects. Bone 1999;25(2 Suppl):55S-58S https://doi.org/10.1016/S8756-3282(99)00098-8
  13. Sigurdsson TJ, Nygaard L, Tatakis DN et al. Periodontal repair in dogs: evaluation of rhBMP-2 carriers. lnt J Periodontics Restorative Dent 1996;16(6):524-537
  14. Choi SH, Kim CK, Cho KS et al. Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP -2/ACS) on healing in 3-wall intrabony defects in dogs. J Periodontol 2002;73(1): 63-72 https://doi.org/10.1902/jop.2002.73.1.63
  15. Kawamura M, Urist MR. Human fibrin is a physiologic delivery system for bone morphogenetic protein. Clin Orthop Relat Res 1988(235):302-310
  16. Wu CH, Hara K, Ozawa H. Enhanced osteoinduction by intramuscular grafting of BMP-beta-TCP compound pellets into murine models. Arch Histol Cytol 1992;55(1): 97-112 https://doi.org/10.1679/aohc.55.97
  17. Han DK, Kim CS, Jung UW et al. Effect of a fibrin-fibronectin sealing system as a carrier for recombinant human bone morphog enetic protein-4 on bone formation in rat calvarial defects. J Periodontol 2005;76(12): 2216-2222 https://doi.org/10.1902/jop.2005.76.12.2216
  18. Ahn SH, Kim CS, Suk HJ et al. Effect of recombinant human bone morphogenetic protein-4 with carriers in rat calvarial defects. J Periodontol 2003;74(6):787-797 https://doi.org/10.1902/jop.2003.74.6.787
  19. Hyun SJ, Han DK, Choi SH et al. Effect of recombinant human bone morphogenetic protein-2, -4, and -7 on bone formation in rat calvarial defects. J Periodontol 2005; 76(10):1667-1674 https://doi.org/10.1902/jop.2005.76.10.1667
  20. Jung UW, Choi SY, Pang EK et al. The effect of varying the particle size of beta tricalcium phosphate carrier of recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects. J Periodontol 2006;77(5):765-772 https://doi.org/10.1902/jop.2006.050268
  21. Pang EK, Im SU, Kim CS et al. Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in a rat calvarial defect model. J Periodontal 2004; 75(10):1364-1370 https://doi.org/10.1902/jop.2004.75.10.1364
  22. Lee YJ, Jung SW, Chae GJ, Cho KS, Kim CS The effect of recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system on bone formation in rat calvarial defects. 대한치주과학회지 2007; 37(2(Suppl.)):397-408
  23. Kim CS, Kim JI, Kim J et al. Ectopic bone formation associated with recombinant human bone morphogenetic proteins-2 using absorbable collagen sponge and beta trical-cium phosphate as carriers. Biomaterials 2005;26(15):2501-2507 https://doi.org/10.1016/j.biomaterials.2004.07.015
  24. Kim CS, Choi SH, Choi BK et al. The effect of recombinant human bone morphogenetic protein-4 on the osteoblastic differentiation of mouse calvarial cells affected by Porphyromonas gingivalis. J Periodontol 2002;73(10):1126-1132 https://doi.org/10.1902/jop.2002.73.10.1126
  25. Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res 1986(205):299-308
  26. Groeneveld EH, Burger EH. Bone morphogenetic proteins in human bone regeneration. Eur J Endocrinol 2000;142(1):9-21 https://doi.org/10.1530/eje.0.1420009
  27. Chen D, Zhao M, Mundy GR. Bone morphogenetic proteins. Growth Factors 2004;22(4):233-241 https://doi.org/10.1080/08977190412331279890
  28. Kuboki Y, Saito T, Murata M et al. Two distinctive BMP-carriers induce zonal chondrogenesis and membranous ossification, respectively; geometrical factors of matrices for cell-differentiation. Connect Tissue Res 1995;32(1-4):219-226 https://doi.org/10.3109/03008209509013699
  29. Miyamoto S, Takaoka K, Ono K. Bone induction in monkeys by bone morphogenetic protein. A trans-filter technique. J Bone Joint Surg Br 1993;75(1):107-110
  30. Scott CK, Hightower JA. The matrix of endochondral bone differs from the matrix of intramembranous bone. Calcif Tissue Int 1991;49(5):349-354 https://doi.org/10.1007/BF02556258
  31. Sampath. TK, Reddi AH. Importance of geometry of the extracellular matrix in endochondral bone differentiation. J Cell Biol 1984;98(6):2192-2197 https://doi.org/10.1083/jcb.98.6.2192
  32. Sampath TK, Muthukumaran N, Reddi AH. Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography. Proc Natl Acad Sci USA 1987;84(20):7109-7113
  33. Pini Prato GP, Cortellini P, Clauser C. Fibrin and fibronectin sealing system in a guided tissue regeneration procedure. A case report. J Periodontol 1988;59(10):679-683 https://doi.org/10.1902/jop.1988.59.10.679