Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The Gingival Tissue Levels of Bone Resorptive Mediators in Human Chronic Periodontitis with Type 2 Diabetes Mellitus

2형 당뇨병환자의 만성 치주염 치은조직에서 골흡수 인자의 발현 양상 비교

  • Kim, Mi-Jung (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Ryu, Sang-Ho (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Park, Jin-Woo (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Suh, Jo-Young (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Lee, Jae-Mok (Department of Periodontology, School of Dentistry, Kyungpook National University)
  • 김미정 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 류상호 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 박진우 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 서조영 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 이재목 (경북대학교 치의학전문대학원 치주과학교실)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purposes of this study were to compare and quantify the expression of MMP-3, $PGE_2$ and IL-6 in the gingival tissues of patients with $type_2$ diabetes mellitus and healthy adults with chronic periodontitis. Material and methods: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1(n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2(n=8) is inflamed gingiva from patients with chronic periodontitis. Group 3(n=8) is inflamed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Westernblotting. The quantification of MMP-3, $PGE_2$ and IL-6 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. Results: 1. The expression levels of MMP-3 were shown highest in group 3 compared to group 1 and 2, and It showed increasing tendency in group 2 and 3. 2. The expressions of $PGE_2$ and IL-6 were shown increasing tendency in group 2 and 3, and It was highest in group 3. 3. As expressions of MMP-3 were increased, $PGE_2$ and IL-6 expressions showed increasing tendency in group 3 than group1 and 2, although there were no proportional relationship. Conclusion: This study demonstrated that the expression levels of MMP-3, $PGE_2$ and IL-6 will be inflammatory markers of periodonta linflamed tissue and DM. It can be assumed that MMP-3 affect to expressions of $PGE_2$ and IL-6 in progression of periodontal inflammation with alveolar bone resorption to type 2 DM.

Keywords

References

  1. Sorsa T, Ding Y, Ingman T et al. Cellular source, activation and inhibition of dental plaque collagenase. J Clin Periodontol 1995; 22:709-717 https://doi.org/10.1111/j.1600-051X.1995.tb00831.x
  2. Uitto VJ, Overall CM, McCulloch C. Proteolytic host cell enzymes in gingival crevice fluid. Periodentol 2000 2003;31:77 -104 https://doi.org/10.1034/j.1600-0757.2003.03106.x
  3. Ejeil AL, Igondjo-Tchen S, Ghomrasseni S et al. Expression of matrix metalloproteinases( MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in healthy and diseased human gingiva. J Periodontol 2003;74:188-195 https://doi.org/10.1902/jop.2003.74.2.188
  4. Woessner JF. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB Journal 1991;5:2145-2154 https://doi.org/10.1096/fasebj.5.8.1850705
  5. Unemori EN, Bair MJ, Bauer EA, Amento EP. Stromelysin expression regulates collagenase activation in human fibroblast. J Biol Chem 1991;266:23477-23482
  6. Domeij H, Yucel-Lindberg T, Modeer T. Signal pathways involved in the production of MMP-1 and MMP-3 in human gingival fibroblasts European Journal of Oral Science 2002;110:302-306 https://doi.org/10.1034/j.1600-0722.2002.21247.x
  7. Nakaya H, Oates TW, Hoang AM, Kamoi K, Cochran DL. Effects of IL-1 beta on matrix metalloproteinase 3 levels in human periodontal ligament cells. J Periodontol 1997; 68:517-523 https://doi.org/10.1902/jop.1997.68.6.517
  8. Suzuki K, Enghild JJ, Morodomi T, Salvesen G, Nagase H. Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin). Biochemistry 1990;29:10261-10270 https://doi.org/10.1021/bi00496a016
  9. Ogata Y, Enghild JJ, Nagase H . Matrix metalloproteinase 3 (stromelysin) activates the precursors for human matrix metalloproteinase 9. J Biol Chem 1992;267:3581-3584
  10. Salvi GE, Brown CE, Fujihashi K et al. Inflammatory mediators of the terminal dentition in adult and early onset periodontitis. J Periodont Res 1998;33:212-225 https://doi.org/10.1111/j.1600-0765.1998.tb02193.x
  11. Nishikawa M, Yamaguchi Y, Yoshitake K, Saeki Y. Effects of TNF$\alpha$ and prostaglandin E2 ont he expression of MMPs in human periodontal ligament fibroblasts. J Periodont Res 2002;37:167-176 https://doi.org/10.1034/j.1600-0765.2002.00656.x
  12. He CS, Wilhelm SM, Pentland AP et al. Tissue cooperation in a proteolytic cascade activating human interstitial collagenase. Proceedings of the National Academy of Sciences USA 1989;86:2632-2636 https://doi.org/10.1073/pnas.86.8.2632
  13. Richards D, Rutherford RB. The effects of interleukin1 on collagenolytic activity and prostaglandin-E secretion by human periodontal- ligament and gingival fibroblasts. Archives of Oral Biology 1988;33:237-243 https://doi.org/10.1016/0003-9969(88)90184-7
  14. Zhong Y, Slade GD, Beck JD, Offenbacher S. Gingival crevicular fluid interleukin- 1beta, prostaglandin E2 and periodontal status in a community population, J Clin Periodontol. 2007;34(4):285-93 https://doi.org/10.1111/j.1600-051X.2007.01057.x
  15. Geivelis M, Turner DW, Pederson ED, Lamberts B. Measurements of interleukin-6 in gingival crevicular fluid from adults with destructive periodontal disease. J Periodontol 1993;64:980-983 https://doi.org/10.1902/jop.1993.64.10.980
  16. Reinhardt RA, Masada MP, Kaldahl WB et al. Gingival fluid IL-1 and IL-6 levels in refractory periodontitis. J Clin Periodontol 1993;20:225-231 https://doi.org/10.1111/j.1600-051X.1993.tb00348.x
  17. Irwin CR, Myrillas TT. The role of IL-6 in the pathogenesis of periodontal disease. Oral Dis 1998;4:43-47 https://doi.org/10.1111/j.1601-0825.1998.tb00255.x
  18. Emrich LJ, Shlossman M, Genco RJ. Periodontal disease in non-insulin dependent diabetes mellitus. J Periodontol 1991; 62:123-130 https://doi.org/10.1902/jop.1991.62.2.123
  19. Taylor GW, Burt BA, Becker MP et al. Non-insulin dependent diabetes mellitus and alveolar bone loss progression over 2 years. J Periodontol 1998;69:76-83 https://doi.org/10.1902/jop.1998.69.1.76
  20. Wilton JMA, Griffiths GS, Curtis MA et al. Detection of high-risk groups and individuals for periodontal diseases. J Clin Periodontol 1988;15:339-346 https://doi.org/10.1111/j.1600-051X.1988.tb01009.x
  21. Olliver RC, Tervonen T. Diabetes-A risk factor for periodontitis in adults? J Periodontol 1994;65:530-538 https://doi.org/10.1902/jop.1994.65.5s.530
  22. Rayfield EJ, Ault MJ, Keusch GT et al. Infection and diabetes, the case for glucose control. Am J Med 1982;72:439-450 https://doi.org/10.1016/0002-9343(82)90511-3
  23. The American Academy of Periodontology. Diabetes and periodontal diseases(positionpaper). J Periodontol 1996;67:166-176
  24. Wilson RM, Reeves WG. Neutrophil phagocytosis and killing ininsulin-dependent diabetes. ClinExpImmunol 1986;63:478-484
  25. Sorsa T, Ingman T, Suomaainen K et al. Cellular source and tetracycline inhibition of gingival crevicular fluid collagenase of patients with labile diabetes mellitus. J Clin Periodontol 1992;19:146-149 https://doi.org/10.1111/j.1600-051X.1992.tb00454.x
  26. Salvi GE, Yalda B, Collins JG et al. Inflammatory mediator response as a potential risk marker for periodontal diseases in insulin-dependent diabetes mellitus patients. J Periodontol 1997;68:127-135 https://doi.org/10.1902/jop.1997.68.2.127
  27. Muhlman HR and Son SH. Gingival bleeding - a leading symptom in initial gingivitis. Helvitica Odontologica Acta 1971;15:107-113
  28. Kang MG, Cha HG, Song SH et al. MMP-2, MMP-8 Expression in gingival tissue of chronic periodontitis associated to Type 2 Diabetes Mellitus. J Korean Aca Periodontol 2005;35:661-674 https://doi.org/10.5051/jkape.2005.35.3.661
  29. Cho JY, Xing S, Liu X et al. Expression and activity of human Na+/I-symporter-inhumangliornacellsbyadenovirus- mediatedgenedelivary. GeneTherapy2000;7:740-749
  30. Brownlee M. Glycation and diabetic complications. Diabetes 1994;43:836-841 https://doi.org/10.2337/diab.43.6.836
  31. Kubota T, Nomura T, Takahashi T, Hara K. Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in periodontitis-affected human gingival tissue. Archives of Oral Biology 1996;41:253-262 https://doi.org/10.1016/0003-9969(95)00126-3
  32. Birkedal-Hansen H. Role of matrix metalloproteinases in human periodontal diseases. J Periodontol 1993;64:474-484
  33. Tuter G, Kurtis B, Serdar M et al. Effects of phase I periodontal treatment on gingival crevicular fluid levels of matrix metalloproteinase- 3 and tissue inhibitor of metalloproteinase-1. J Clin Periodontol 2005; 32:1011-1015 https://doi.org/10.1111/j.1600-051X.2005.00816.x
  34. Alpagot T, Bell C, Lundergan W, Chambers DW, Rudin R. Longitudinal evaluation of GCF MMP-3 and TIMP-1 levels as prognostic factors for progression of periodontitis. J Clin Periodontol 2001;28:353-359 https://doi.org/10.1034/j.1600-051x.2001.028004353.x
  35. Choi BK, Moon SY, Cha JH, Kim KW, Yoo YJ. Prostaglandin E2 is a main mediator in receptor activator of nuclear factor-kB ligand- dependent osteoclastogenesis induced by Porphyromonas gingivalis, Treponema denticola, and Treponema socranskii. J Periodontol 2005;76:813-820 https://doi.org/10.1902/jop.2005.76.5.813
  36. Ishimi Y, Miyaura C, Jin CH et al. IL-6 is produced by osteoblasts and induces bone resorption. J Immunol 1990;145:3297-3303