Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of Follicle Stimulating Hormone and Human Chorionic Gonadotrophin on the In Vitro Maturation of Canine Oocytes

  • Kim, Min-Kyu (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Oh, Hyun-Ju (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Hong, So-Gun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jung-Eun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Hye-Jin (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Hyung-Suk (Woosong Information College) ;
  • Kim, Sang-Cheol (Department of Animal Science, Shingu College) ;
  • Kang, Sung-Keun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong-Chun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
  • Published : 2007.03.31
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Abstract

The present study investigated the effects of follicle stimulating hormone (FSH) and human chorionic gona-dotrophin (hCG) on the nuclear maturation of canine oocytes. Oocytes were recovered from mongrel female ovaries in various reproductive states; follicular, luteal or anestrous stage. Oocytes were cultured in sserum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.5, 1.0 or 10 IU) or hCG (Exp.2:0, 0.5, 1.0 or 10 IU) or both (Exp. 3:1 IU FSH +1 IU hCG) for 72 hr to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting. The denuded oocytes were stained with $1.9\;{\mu}M$. Hoechst 33342 in glycerol and the nuclear state of oocytes was evaluated under UV light. More (p<0.05) oocytes matured to MII stage when follicular stage oocytes were supplemented with 1 IU FSH (6.2%) compared with the control, 0.1 or 10.0 IU FSH (0 to 1.2%). Significantly higher (p<0.05) maturation rate to MII stage was observed in follicular stage oocytes supplemented with 1.0 IU hCG (7.2%) compared with the control or other hCG supplemented groups (0 to 1.5%). However, the combination of FSH and hCG did not improve the nuclear maturation rate of canine oocyte (2.4 %) compared with FSH (6.2%) and hCG alone (7.2%). In conclusion, FSH or hCG alone significantly increased the maturation of canine oocytes to MII stage.

Keywords

References

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