Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Physiological Response and Hematological Characteristics of Goldfish (Carassius auratus) to Water Temperature Shock

수온충격에 따른 금붕어(Carassius auratus)의 혈액 성상 및 생리적 반응

  • Hur, Jun Wook (School of Marine Life Science, Kunsan National University) ;
  • Habibi, Hamid R. (Department of Biological Sciences, University of Calgary)
  • 허준욱 (군산대학교 해양생명과학부) ;
  • Received : 2007.03.14
  • Accepted : 2007.05.28
  • Published : 2007.06.30
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Abstract

The effects of sudden changes of water temperature (WT) on the physiological response and hematological characteristics of the goldfish (Carassius auratus) were examined by manipulating WT in a flow through freshwater culture system with tanks. The WT was dropped from $15^{\circ}C$ to $10^{\circ}C$ within 1 hour and then returned to the original water temperature within 12 hours and maintained for 12 hours at the normal WT. The WT stress give continued for 3 days. Plasma levels of cortisol, glucose and lactic acid were higher in stress group than that of non-stressed group until 72 hours. However, The $Na^+,\;Cl^-$, osmolality and aspartate aminotransferase (AST) levels showed no significant differences in two groups. This results in stress group showed that goldfish exhibit "typical" physiological responses (in cortisol, glucose, lactic acid, hematocrit, red blood cell and hemoglobin) to the stress induced by WT changes.

금붕어(Carassius auratus)를 사용하여 수온변화에 따른 스트레스 반응에 대한 기초자료를 얻고자, 수온상승과 하강에 따른 혈액 성상 및 생리적 반응을 조사하였다. 수온은 $15^{\circ}C$로부터 급격하게 하강시켜 1시간이내에 $10^{\circ}C$로 사육수를 조절하였으며, 이후 자연적으로 수온이 상승하도록 조절하였다. 12시간째에 수온은 $15^{\circ}C$로 회복되어 24시간째까지 유지하였다. 이러한 수온자극 스트레스를 3일 연속하여 주었다. 혈장 cortisol, glucose 및 lactic acid에서 72시간까지 대조구보다 스트레스 실험구에서 유의적으로 높은 값을 보였다. 혈장 $Na^+,\;Cl^-$ 및 aspartate aminotransferase (AST)는 두 그룹에서 유의적 차이를 보이지 않았다. 본 연구결과 혈장 cortisol, glucose, lactic acid, hematocrit, red blood cell 및 hemoglobin에서 일반적인 스트레스 반응을 보였다.

Keywords

Acknowledgement

Supported by : 한국학술진흥재단

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