Role of Protein Kinase C in Abnormal Proliferation of Vascular Endothelial Cell induced by 1,2-Dimethylhydrazine; Analysis of Isoform

디메틸히드라진(1,2-Dimethylhydrazine)으로 유도된 혈관내피세포의 비정상적인 증식에서 단백활성효소 시이(Protein Kinase C)의 역할; 동종효소 분석

  • Lee, Jin (Lee Jin Aesthetic Clinic) ;
  • Bae, Yong Chan (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University) ;
  • Park, Suk Young (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University) ;
  • Moon, Jae Sul (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University) ;
  • Nam, Su Bong (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University)
  • 이진 (이진 성형외과) ;
  • 배용찬 (부산대학교 의과대학 성형외과학교실) ;
  • 박숙영 (부산대학교 의과대학 성형외과학교실) ;
  • 문재술 (부산대학교 의과대학 성형외과학교실) ;
  • 남수봉 (부산대학교 의과대학 성형외과학교실)
  • Received : 2006.05.19
  • Published : 2007.01.10


Purpose: Protein tyrosine kinase(PTK), protein kinase C(PKC), oxidase, as a mediator, have been known to take a role in signal transduction pathway of angiogenesis. The authors confirmed that PKC is the most noticeable mediator for abnormal proliferation of vascular endothelial cells through in vitro study model using the inhibitors, targeting the formation of three co-enzymes. In this study, we would investigate which isoform of PKC play an important role in abnormal angiogenesis of vascular endothelial cell. Methods: In 96 well plates, $10^4$ HUVECs(human umbilical vein endothelial cells) were evenly distributed. Two groups were established; the control group without administration of DMH(1,2-dimethylhydrazine) and the DMH group with administration of $7.5{\times}10^{-9}M$ DMH. RNA was extracted from vascular endothelial cell of each group and expression of the PKC isoform was analyzed by RT-PCR(reverse transcriptase-polymerase chain reaction) method. Results: RT-PCR analysis showed that $PKC{\alpha}$, $-{\beta}I$, $-{\beta}II$, $-{\eta}$, $-{\mu}$ and $-{\iota}$ were expressed in vascular endothelial cells of each group. DMH incresed the expression of $PKC{\alpha}$ and $PKC{\mu}$, and decreased $PKC{\beta}I$, $PKC{\beta}II$ expression dominantly. Conclusion: Based on the result of this study, it was suggested that $PKC{\alpha}$ and $PKC{\mu}$ may have significant role in abnormal proliferation of vascular endothelial cell.



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