Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Study on the confirmation of drinking at the bloods & urines used 5-hydroxyindole-3-acetic acid and 5-hydroxytryptophol

5-Hydroxyindole-3-acetic acid와 5-hydroxytryptophol을 이용한 혈액 및 뇨에서 음주여부 확인에 관한 연구

  • Received : 2007.04.05
  • Accepted : 2007.05.28
  • Published : 2007.06.28
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Abstract

The study was carried out to investigate the ratio of ethanol to n-propanol in blood and urine specimens, and developed a method for distinguishing ingested ethanol from artifactual ethanol in urine samples. In case of no urinary ethanol was detected, the ratio of ethanol to n-propanol concentration was about 12~20 times higher than those of blood. Therefore, it might be a good method to determine whether the detected ethanol is from drinking or from microbial fermentation. During the metabolism of ethanol, the levels of the metabolite of serotonin (5-hydroxytryptamine, 5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) were decreased, while 5-hydroxytryptophol (5-HTOL) was increased. The levels of 5-HTOL/5-HIAA in urine samples of drinking suspects were greater than 1, in that of no drinking suspects were less than 1.

사망사건 중 부패된 생체시료(혈액, 뇨 등)에서 음주여부를 판단하고자 할 때 부패된 사체의 경우 glucose를 비롯한 생체물질들이 미생물에 의하여 alcohol로 대사전환 될 수 있으므로 검출되는 ethanol의 전체 농도를 사망 전의 음주량으로 판단할 수 없다. 따라서 본 실험에서는 감정 의뢰된 사체의 혈액과 뇨를 임의로 선택하여 ethanol의 농도 및 n-propanol의 농도를 측정하여 상대적인 생성 비를 비교하여 보았으며, 5-hydroxytryptophol(5-HTOL)/5-hydroxyindole-3-acetic acid(5-HIAA)의 비를 이용한 음주여부의 판별법을 확립하기 위해서 5-HIAA 및 5-HTOL의 농도 측정법을 확립하였다. 혈액에서 검출되는 ethanol과 n-propanol의 상대적 농도비는 뇨에서 ethanol이 검출되지 않은 시료를 기준으로 할 경우, 약 11~20:1의 농도비를 나타난다. 음주를 한 것으로 판단되는 뇨의 경우 5-HTOL/5-HIAA의 비율은 1 이상으로 나타났고, 음주를 하지 않은 것으로 판단되는 뇨의 경우 5-HTOL/5-HIAA의 비율이 1 미만으로 측정되어, 혈액검사와는 달리 5-HTOL/5-HIAA의 비율이 1 이상인 경우 음주를 한 것으로 간주할 수 있었다.

Keywords

References

  1. Widmark, 'Priciples and Applications of Medicolegal Alcohol Determination', Davis, Cali. (1981)
  2. 유영찬, '법과학', 도서출판 신일상사 (1991)
  3. A. W. Culp, 'Principle of energy conversion', McGraw- Hill Book, Inc. (1991)
  4. J.P. Framke, 'Systermatic Analysis of Solvents and Other Volatile Substances by GC', J.O.A.T., 12, 20 (1988)
  5. C.J. Naresh, 'Direct Blood Injection Method for GC Determination of Alcohols and other Volitile Compounds', Clinical Chemistry, 17, 2 (1971)
  6. C. James, Garriott, 'Medicolegal Aspects of Alcohol', Lawyers & Judges Pubilshing Co., third edition (1996)
  7. K. M. Dubowski, 'Manual for Analysis of Ethanol in Biological Liquids', U.S. Dept. of Transportation, 5 (1977)
  8. 박성우, 김동욱, '생체시료중에서 Ethylalcohol의 분포에 관한 연구', 국과수 연보, 25, 233 (1993)
  9. S. Krishnaswamy, Q. Hao, L.L. Von Moltke, D.J. Greenblatt & M.H. Court, 'Evaluation of 5-hydroxytryptophol and dther endogenous serotonin (5-5-hydroxytryptamine) analogs as substrates for UDP-glucuronosyltransferase 1A6', National Library of Medicine, 32(8), 862-869 (2004)
  10. Magnus Huss Klinik al., 'Studies on 5-hydroxytryptophol and the meta-bolic interaction between serotonin and ethanol', KAROLINSKA INSTITUTET (1999)
  11. V. E. Davis, H. Brown, J. A. Huff, J. L. Cashaw, 'The alteration of serotonin metabolism to 5-hydroxytryptophol by ethanol ingestion in man', J. Lab. Clin. Med. 69, 132 (1967)
  12. A. Helander, O. Beck, G.. Jacobsson, C. Lowenmo, and T. Wikstrom, 'Time course of Ethanol-Induced Changes in Serotonin Metabolism', Life Sciences, 53, 847 (1993)
  13. Anders Helander and Lars Boysen, '5-Hydroxytryptophol conjugation in man: influence of alcohol consumption and altered serotonin turnover', Life Sciences, 56, pp1529-1534 (1995) https://doi.org/10.1016/0024-3205(95)00115-M
  14. Taisto Sarkola, Helen Dahl, C.J. Peter Eriksson and Anders Helander, 'Urinary ethyl glucuronide and 5- hydroxytryptophol levels during repeated ethanol ingestion in healthy human subjects', Alcohol and Alcoholism, 38(4), pp347-351 (2003) https://doi.org/10.1093/alcalc/agg083